Purification and physico-chemical characterization of Ecbo-virus type SA-I

Ecbovirus type SA-l, a bovine enterovirus, has been purified and crystallized and its chemical composition, physical characteristics and morphology studied. The virus particles have a mean diameter of 230Å, a sedimentation constant of 152S, a diffusion constant of approximately 1.7 x 10⁻⁷ cm² sec⁻¹ and a particle weight of about 6.1 x 10⁶ daltons. Its capsid consists of a number of identical subunits, probably 32, arranged in icosahedral symmetry and enclosing the nucleic acid component which comprises about 30 per cent of the total weight. Infective ribonucleic acid could only be isolated from infected cells. It has a sedimentation constant of approximately 37S corresponding to a molecular weight of about 2 x 10⁶, and consists of single-stranded RNA. The presence of a small amount of infective double-stranded RNA could also be demonstrated. In its physical characteristics and appearance, therefore, the virus is practically indistinguishable from polio- and other enteroviruses. The small differences in size found by different authors for various enteroviruses are probably insignificant and due to experimental error.The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.ab201

The serological relationship of South African bovine enterovirus strains (Ecbo SA-I and-II) and the growth characteristics in cell culture of the prototype strain (Ecbo SA-I)

A locally isolated bovine enterovirus designated echovirus SA-I was found to belong to the Weybridge serotype 134. The growth characteristics of the virus in a number of cell types were studied. Its replicative cycle and its effect on the metabolism of the host cell, studied by means of isotope-labelling techniques, closely resemble those of other enteroviruses. It was shown that the inhibitors of cellular RNA and protein synthesis are produced normally even if replication of the infecting virus is blocked by guanidine.The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

History of bluetongue research at Onderstepoort : transboundary diseases

Research on this economically important disease of ruminants, especially sheep, which had been named bluetongue by farmers in the 19th century, has been part and parcel of the activities at Onderstepoort ever since its establishment in 1908 and therefore covers a full century of the OVI's existence. In view of Onderstepoort's centenary celebration a brief overview of this research is given in terms of the historic milestones which influenced and guided global research on this and other viral diseases of animals

A brief historical review of research achievements by the OVI during the 20th century against the background of socioeconomic and political developments in South Africa : information article

Journal of the South African Veterinary Association, Vol 71, Issue 4, De

Chicken single-chain antibody fragments directed against recombinant VP7 of bluetongue virus

VP7, the major structural core protein of bluetongue virus, is conserved among the 24 bluetongue virus serotypes. The gene encoding VP7 of serotype 4 was expressed in Escherichia coli. A semi-synthetic chicken antibody library was screened with the resulting protein. Six single-chain antibody fragments (scFvs) were isolated. Immune sera blocked the binding of four of the six scFvs in enzyme-linked immunosorbent assays. These scFvs recognised recombinant VP7 coated directly onto a plastic surface. Their behaviour therefore differs from that of scFv F10 which was selected earlier on directly immobilised bluetongue virus and which binds to VP7 only when it is captured by an immobilised immunoglobulin directed against bluetongue virus.Department of Science and Technologyhttp://www.tandfonline.com/loi/cfai20ab201