The environmental impact of the reconstruction and development of the coastal marine transport infrastructure

The coastal marine transport is one of the important components defining development of all Black Sea coast of Russia. Questions of need of restoration of coastal passenger traffic within the coast of Greater Sochi in connection with intensive development of tourism in Krasnodar Region of Russia and the questions of the organization of the transport communication between a coastal towns and settlements are considered. Need of further implementation of the program of reconstruction and construction of port points (moorings) and objects of coastal transport infrastructure in the Sochi region and in all Black Sea coast of Russia is shown. The analysis of a problem of environmental protection, which can arise at implementation of the project of reconstruction and development of coastal sea transport infrastructure in the Sochi region, is made. Possible ways of their solution for reduction of impacts on the environment are proposed. Introduction of the program of development of objects of coastal transport in the region, taking into account environment will contribute to sustainable development of the region

Inflammatory pain and corticosterone response in infant rats: effect of 5-HT1A agonist buspirone prior to gestational stress

Our researches have shown that gestational stress causes exacerbation of inflammatory pain in the offspring; the maternal 5-HT1A agonist buspirone before the stress prevents the adverse effect. The serotonergic system and hypothalamo-pituitary-adrenal (HPA) axis are closely interrelated. However, interrelations between inflammatory pain and the HPA axis during the hyporeactive period of the latter have not been studied. The present research demonstrates that formalin-induced pain causes a gradual and prolonged increase in plasma corticosterone level in 7-day-old male rats; twenty-four hours after injection of formalin, the basal corticosterone level still exceeds the initial basal corticosterone value. Chronic treatments of rat dams with buspirone before restraint stress during gestation normalize in the offspring pain-like behavior and induce during the acute phase in the formalin test the stronger corticosterone increase as compared to the stress hormonal elevation in animals with other prenatal treatments. Negative correlation between plasma corticosterone level and the number of flexes+shakes is revealed in buspirone+stress rats. The new data enhance the idea about relativity of the HPA axis hyporeactive period and suggest that maternal buspirone prior to stress during gestation may enhance an adaptive mechanism of the inflammatory nociceptive system in the infant male offspring through activation of the HPA axis peripheral link

Long-term effects of chronic buspirone during adolescence reduce the adverse influences of neonatal inflammatory pain and stress on adaptive behavior in adult male rats

Neonatal pain and stress induce long-term changes in pain sensitivity and behavior. Previously we found alterations in pain sensitivity in adolescent rats exposed to early-life adverse events. We tested whether these alterations have long-lasting effects and if those effects can be improved by the 5-hydroxytryptamine 1A (5-HT1A) receptor agonist buspirone injected chronically during the adolescent period. This study investigates: (1) effects of inflammatory pain (the injection of formalin into the pad of a hind paw) or stress (short maternal deprivation-isolation, MI), or their combination in 1â\u80\u932-day-old rats on the adult basal pain, formalin-induced pain, anxiety and depression; (2) effects of adolescent buspirone in adult rats that experienced similar early-life insults. Changes in nociceptive thresholds were evaluated using the hot plate (HP) and formalin tests; levels of anxiety and depression were assessed with the elevated plus maze and forced swim tests respectively. Both neonatal painful and stressful treatments induced long-term alterations in the forced swim test. Other changes in adult behavioral responses were dependent on the type of neonatal treatment. There was a notable lack of long-term effects of the combination of early inflammatory pain and stress of MI on the pain responses, anxiety levels or on the effects of adolescent buspirone. This study provides the first evidence that chronic injection of buspirone in adolescent rats alters antinociceptive and anxiolytic effects limited to adult rats that showed behavioral alterations induced by early-life adverse treatments. These data highlight the role of 5-HT1A receptors in long-term effects of neonatal inflammatory pain and stress of short MI on adaptive behavior and possibility of correction of the pain and psychoemotional behavior that were altered by adverse pain/stress intervention using buspirone during critical adolescent period

ПРИНЦИПЫ ФОРМИРОВАНИЯ ИННОВАЦИОННОЙ МОДЕЛИ КОНТРОЛЯ НАД ЭФФЕКТИВНОСТЬЮ ИСПОЛЬЗОВАНИЯ БЮДЖЕТНЫХ СРЕДСТВ

The article describes the innovative approach to use of performance indicators to improve the quality of efficiency control of budget funds use, it is offered to create the inner budgetary administrative system of control on the effectiveness and appropriateness of the budgetary funds use.В статье рассмотрен инновационный подход по применению оценочных показателей для повышения качества контроля над эффективностью использования бюджетных средств, предлагается создать внутри бюджетную административную систему контроля по эффективности и целесообразности использования бюджетных средств

CX3CL1 release during immunogenic apoptosis is associated with enhanced anti-tumour immunity

IntroductionImmunogenic cell death (ICD) has emerged as a novel option for cancer immunotherapy. The key determinants of ICD encompass antigenicity (the presence of antigens) and adjuvanticity, which involves the release of damage-associated molecular patterns (DAMPs) and various cytokines and chemokines. CX3CL1, also known as neurotactin or fractalkine, is a chemokine involved in cellular signalling and immune cell interactions. CX3CL1 has been denoted as a “find me” signal that stimulates chemotaxis of immune cells towards dying cells, facilitating efferocytosis and antigen presentation. However, in the context of ICD, it is uncertain whether CX3CL1 is an important mediator of the effects of ICD.MethodsIn this study, we investigated the intricate role of CX3CL1 in immunogenic apoptosis induced by mitoxantrone (MTX) in cancer cells. The Luminex xMAP technology was used to quantify murine cytokines, chemokines and growth factors to identify pivotal regulatory cytokines released by murine fibrosarcoma MCA205 and melanoma B16-F10 cells undergoing ICD. Moreover, a murine tumour prophylactic vaccination model was employed to analyse the effect of CX3CL1 on the activation of an adaptive immune response against MCA205 cells undergoing ICD. Furthermore, thorough analysis of the TCGA-SKCM public dataset from 98 melanoma patients revealed the role of CX3CL1 and its receptor CX3CR1 in melanoma patients.ResultsOur findings demonstrate enhanced CX3CL1 release from apoptotic MCA205 and B16-F10 cells (regardless of the cell type) but not if they are undergoing ferroptosis or accidental necrosis. Moreover, the addition of recombinant CX3CL1 to non-immunogenic doses of MTX-treated, apoptotically dying cancer cells in the murine prophylactic tumour vaccination model induced a robust immunogenic response, effectively increasing the survival of the mice. Furthermore, analysis of melanoma patient data revealed enhanced survival rates in individuals exhibiting elevated levels of CD8+ T cells expressing CX3CR1.ConclusionThese data collectively underscore the importance of the release of CX3CL1 in eliciting an immunogenic response against dying cancer cells and suggest that CX3CL1 may serve as a key switch in conferring immunogenicity to apoptosis

Grounds of divorce in Russia and France: a Comparative Legal Analysis

This article examines the legal regulation of the Institute of divorce in Russia and France, and carries out a comparative legal analysis of the grounds for divorce

Statutory Regulation of the Unworthy Heirs Institute in Russia, France and Spain: a Comparative Legal Analysis

In the paper, the authors analyze the institute of unworthy heirs in Russia, France and Spain, carry out a comparative legal analysis of classes of unworthy heirs and consider issues in the Russian Civil Code regulation

Reorganization through Consolidation and Merger of Joint Stock Companies in Russia and Business Corporations in the USA: Comparative Legal Analysis

The article presents a comparative analysis of the reorganization of joint stock companies in the Russian Federation and business corporations in the USA (state of Delaware) through consolidation and merger: it describes legal regime of the reorganization and outlines stages related to the reorganizational procedures under the Russian and USA law

The environmental impact of the reconstruction and development of the coastal marine transport infrastructure

The coastal marine transport is one of the important components defining development of all Black Sea coast of Russia. Questions of need of restoration of coastal passenger traffic within the coast of Greater Sochi in connection with intensive development of tourism in Krasnodar Region of Russia and the questions of the organization of the transport communication between a coastal towns and settlements are considered. Need of further implementation of the program of reconstruction and construction of port points (moorings) and objects of coastal transport infrastructure in the Sochi region and in all Black Sea coast of Russia is shown. The analysis of a problem of environmental protection, which can arise at implementation of the project of reconstruction and development of coastal sea transport infrastructure in the Sochi region, is made. Possible ways of their solution for reduction of impacts on the environment are proposed. Introduction of the program of development of objects of coastal transport in the region, taking into account environment will contribute to sustainable development of the region