Comparison of transduction efficiency of serotype AAV2/1, AAV2/5 and AAV2/8 in the VMH.

<p>Rats (n = 6) were injected with 1×10⁹ genomic copies of AAV2/1, AAV2/5 or AAV2/8 in the VMH. The transduced area was identified using ISH on GFP. AAV2/1 and AAV2/5 were equally efficient in transducing the VMH (A, B, D) and performed significantly better than AAV2/8 (C, D) (P<0.01 and P<0.05, respectively). The VMH area is indicated by a dotted line. Figure E depicts the hypothalamic area. The square indicates the area that is enlarged in Figures A, B and C. mt  =  mammillary tract; f  =  fornix; dmh  =  dorsomedial hypothalamus; vmh  =  ventromedial hypothalamus. Scale bar: 500 µm.</p

Blood clearance kinetics of AAV2/1, AAV2/2, AAV2/5 and AAV2/8 after systemic administration.

<p>Rats (n = 6) received a systemic injection of 1×10⁹ AAV2/1, AAV2/2, AAV2/5 or AAV2/8 and blood samples were collected 10 m, 20 m, 40 m, 1 h, 4 h and 24 h after injection. AAV2/2 showed a significantly faster clearance rate than AAV2/1, AAV2/5 and AAV2/8. Four hours after injection, less than 3% of the starting material of AAV2/1 and AAV2/2 could be traced. AAV2/8 showed a delayed clearance rate. One day after injection still more than 20% of the starting material was present in the circulation.</p