5 research outputs found
Top 20 pharmaceutical perturbagens exhibiting positive correlation to the gene signature induced by Cud C treatment.
<p>Top 20 pharmaceutical perturbagens exhibiting positive correlation to the gene signature induced by Cud C treatment.</p
Cudraflavone C inhibits PI3K activity.
<p>The effect of negative control (1%DMSO), Cud C or LY-294002 (100 Ī¼M) on p110Ī±/p85Ī±, p110Ī²/p85Ī±, p110Ī“/p85Ī±, and p120Ī³ PI3K activity were quantified using the PI3K-Glo<sup>ā¢</sup> Class I Profiling Kit. All data represents the mean Ā± s.d. from at least three independent experiments. Symbol ā*ā presents the statistical significance concluded from Studentās independent <i>t</i>-test with p-value ā¤0.05.</p
Cudraflavone C induces tumor-specific cell death in colorectal cancer cells.
<p>(A) Chemical structure of Cud C. (B) KM12, HT29, Caco-2, HCC2998, HCT116 and SW48 colorectal cancer cells were exposed to various concentrations of Cud C for 72 hours. Cell viability was recorded using CellTitre Glo<sup>Ā®</sup> luminescence assay. (C) KM12, Caco-2 and CCD 841 CoN were treated with 0.1% DMSO (control) or 10Ī¼M Cud C (Cud C) for 72 hours followed by microscopy analysis (Ć100 magnification). (D) Apoptotic cell death in KM12, Caco-2 and CCD 841 CoN cells was quantified using Annexin V/7-AAD flow cytometry at 72 hours following treatment. (E) Caspase activities in KM12 and Caco-2 cells were assessed by Caspase Glo<sup>Ā®</sup> assay at 72 hours following treatment. (F) 10Ī¼M Cud C induced mitochondrial membrane depolarization. Caco-2 and KM12 cells stained with JC-1 at 72 hours after treatment with Cud C. The green dye represents JC-1 monomers in cytoplasm while the red dye represents JC-1 aggregates in nucleus. Cells were observed under fluorescence microscope (Ć100 magnification). All data represent the mean Ā± s.d. from at least three independent experiments. Symbol ā*ā presents the statistical significance concluded from Studentās independent <i>t</i>-test with p-value ā¤0.05.</p
IC<sub>50</sub> of Cud C and 5-fluorouracil in colorectal cancer and non-transformed colon epithelial cells.
<p>IC<sub>50</sub> of Cud C and 5-fluorouracil in colorectal cancer and non-transformed colon epithelial cells.</p
Differential gene expression regulated by cudraflavone C in Caco-2 cells.
<p>(A) Heatmaps generated based on the genes regulated by Cud C. Caco-2 cells were exposed to 10 Ī¼M Cud C for 48 hours. GeneChip<sup>Ā®</sup> Human Transcriptome Array 2.0 (Affymetrix, USA) was applied. Gene expression changes that ā„2-fold were considered significant. Control 1 and Control 2 represent gene expression from cells treated with vehicle control (1% DMSO); Cud C 1 and Cud C 2 were gene expression from cells treated with Cud C (10 Ī¼M). (B) qPCR was used to validate the microarray data. Caco-2 cells were exposed to 10 Ī¼M Cud C for 12, 24, 48 or 72 hours The left and right panels present genes that are up and down-regulated respectively. All data represent the mean Ā± s.d. from at least three independent experiments. Symbol ā*ā indicates the statistical significance concluded from Studentās independent <i>t</i>-test with p-value ā¤0.05.</p