4 research outputs found
An <i>MtnB-eYFP</i> reporter (green fluorescent signal) indicates relative copper and zinc content in midguts from dissected third instar larvae.
<p>Larvae were reared on normal food (NF) or food containing 1 mM Cu or Zn. Expression of eYFP was inhibited by addition of 0.1 mM Ha44. Red arrows indicate regions with lower eYFP expression. Pictures of every condition were taken with light (top) and fluorescence filter (bottom).</p
Dose response effect of Ha44 on larval development.
<p>Concentration of Ha44 (mM) to which first instar larvae were exposed in function of mortality (50 larvae/condition). For each concentration the average mortality (percentage) is presented±S.E. The legend below the graph displays the LD-50 and −90 values (mM) for Ha44 at a 95% significance level according to Probit.</p
Effect of different metals on hatching of Ha44-exposed embryos.
<p>Freshly laid embryos (0–2 hours) were exposed to a lethal dose of Ha44 in the absence/presence of metals [Cu, Zn, Fe(II) and Fe(III)] at different concentrations (mM). Possible significant differences in potency between the different metals were assessed using a two-way ANOVA model combined with a Bonferroni post hoc test (*** = p<0.0001, n.s. = not significant).</p
<i>In vitro</i> inhibitory potency of Ha44:
<p><b>Panel (A).</b> zymogram. Lane 1 and 2 are controls, in lane 3 active meprin 1A is visualised (148 KDa) and lane 4 displays the inhibitory effect of Ha44 on meprin 1A. <b>Panel (B):</b> azocasein (1%) assay. Inhibitory effect (%) of Ha44, TPEN and EDTA (1 mM) on activated meprin 1A compared to a control condition (0% inhibition).</p