4 research outputs found
Microspectrophotometric Determination of DNA Quantities and Polypliody in Fraxinus america L.
Quantitative determination of DNA amounts per cell for
66 Fraxinus americana trees, from 22 locations, was accomplished
by Feulgen microspectrophotometric methods. Measurements
of arbitrary photometric units of relative absorbance
of the DNA-Feulgen dye complex were adjusted to estimates of
actual DNA amounts by the use of chicken erythrocyte nuclei.
A direct correlation was found between observed DNA amounts
and diploid, tetraploid and hexaploid levels. A deviation
of observed ploidy DNA values from a direct multiplication
factor suggests that aneuploidy may be operating in the
population adaptation processes. South of 40° north latitude
both diploid and polyploid individuals were represented
while north of 40° north latitude only diploid individuals
were found