Purification de l'endonucléase d'Escherichia coli spécifique des sites apuriniques de l'ADN.

peer reviewe

COMPLEMENTARITY BETWEEN <i>IN SITU</i> STUDIES AND PHOTOGRAMMETRY: METHODOLOGICAL FEEDBACK FROM A ROMAN SHIPWRECK IN CAESAREA, ISRAEL

As a quick and effective way to archive the different stages of an excavation - notably to prepare the post-excavation phase and to document the production methods – photogrammetry has become an indispensable tool. Indeed, it offers a valid scientific model, usable by any member of the team and at any moment, without the need to return to the excavation site. Photogrammetry can also complement other archaeological tools such as manual surveys. The interaction between the complementary approach of the interpretative drawing measurements (IDM) and the photogrammetric model measurements (PMM) enables us to apprehend the error rate of the interpretative measurements in situ. It appears thus that the measurements taken flat have an error rate inferior to 2% whereas the distances that are either too long or taken on a three-dimensional support have an error rate that can exceed 10%. The input of photogrammetry is therefore an added value whether it be during the excavation phase or during the post-excavation studies

EKSTRAKSI KARAGINAN RUMPUT LAUT MERAH (Kappaphycus alvarezii) DENGAN PERLAKUAN PERENDAMAN DALAM LARUTAN BASA

Semi-refined carrageenan are a type of carrageeanan product that have a low level of purity because it still contains a small amount of selulose within the carageenan. The purpose of this study is to find out the effect of the concentration of both NaOH and KOH towards rendemen, and the physical and chemical charactheristic of semi refined carrageenan made from kappaphycus alvarezii seaweed,  and also to minimize the use of chemical product on SRC production process. The method used in this study is steaming method. The results are the rendemen from NaOH is 10% and KOH 14%. This proves that the concentration of alkali affects the amount of rendemen. The higher the amount of alkali used, the higher the amount of rendemen obtained.  Water content obtained from the NaOH samples are 3,75%; while those from the KOH samples are 5%.  The ash content of semi-refined carrageenan obtain from NaOH samples are 55,42% and KOH are 55,27%. For the pH level on semi-refined carrageenan obtain from the NaOH samples are 8,06; and KOH are 8,69. The alkali concentration  greatly affects the amount of rendemen that is obtained because a higher concentration of alkali during the alkalization process will result in higher pH level, therefore the extration ability of alkali are increased

A new generic open pit mine planning process with risk assessment ability

Conventionally, mining industry relies on a deterministic view, where a unique mine plan is determined based on a single resource model. A major shortfall of this approach is the inability to assess the risk caused by the well-known geological uncertainty, i.e. the in situ grade and tonnage variability of the mineral deposit. Despite some recent attempts in developing stochastic mine planning models which have demonstrated promising results, the industry still remains sceptical about this innovative idea. With respect to unbiased linear estimation, kriging is the most popular and reliable deterministic interpolation technique for resource estimation and it appears to remain its popularity in the near future. This paper presents a new systematic framework to quantify the risk of kriging-based mining projects due to the geological uncertainties. Firstly, conditional simulation is implemented to generate a series of equally-probable orebody realisations and these realisations are then compared with the kriged resource model to analyse its geological uncertainty. Secondly, a production schedule over the life of mine is determined based on the kriged resource model. Finally, risk profiles of that production schedule, namely ore and waste tonnage production, blending grade and Net Present Value (NPV), are constructed using the orebody realisations. The proposed model was applied on a multi-element deposit and the result demonstrates that that the kriging-based mine plan is unlikely to meet the production targets. Especially, the kriging-based mine plan overestimated the expected NPV at a magnitude of 6.70% to 7.34% (135 M$to 151 M$). A new multivariate conditional simulation framework was also introduced in this paper to cope with the multivariate nature of the deposit. Although an iron ore deposit is used to prove the concepts, the method can easily be adapted to other kinds of mineral deposits, including surface coal mine

Optimised diets for achieving One Health: A pilot study in the Rhine-Ruhr Metropolis in Germany

Dietary changes are needed to align the global food systems with the planetary boundaries and contribute to Sustainable Development Goals. We employed a Life Cycle Assessment (LCA) framework, extended with indicators on human health and animal welfare, to assess 2020 food consumption data of a pilot sample collected through an online survey in the Rhine-Ruhr Metropolis (Germany). Feasible optimisation scenarios representing alternative sustainable choices towards overarching environmental, societal and policy goals were explored. Meat and meat products contributed most to overall environmental impacts (e.g., climate change, terrestrial acidification), and fish and seafood to animal welfare loss (e.g., animal lives lost, animal life years suffered). Sodium intake was the most contributing risk factor for life minutes lost. The combined optimisation scenario reduces 55% of greenhouse gas emissions, improves human health indicators by 25% and reduces animal welfare loss substantially (by 52-97%). This is possible with a shift towards flexitarian and vegetarian dietary scenarios. These optimisations deliver improvements across One Health dimensions with marginal changes in dietary scenarios and align with the sustainability goals of the EU Green Deal. Working with regional data can offer advantages in obtaining more realistic baseline dietary information to promote localised dietary shifts. While this research has limitations regarding sample representativeness, it can serve as a case study to encourage sustainable consumption in the Rhine-Ruhr region

Digital Signal Processing Research Program

Contains table of contents for Section 2, an introduction and reports on fourteen research projects.U.S. Navy - Office of Naval Research Grant N00014-91-J-1628Defense Advanced Research Projects Agency/U.S. Navy - Office of Naval Research Grant N00014-89-J-1489MIT - Woods Hole Oceanographic Institution Joint ProgramLockheed Sanders, Inc./U.S. Navy Office of Naval Research Contract N00014-91-C-0125U.S. Air Force - Office of Scientific Research Grant AFOSR-91-0034U.S. Navy - Office of Naval Research Grant N00014-91-J-1628AT&T Laboratories Doctoral Support ProgramNational Science Foundation Fellowshi