Distribution and ecological role of selected shrubby species in the Mediterranean basin

In many natural and semi-natural habitats trees and shrubs represent two major growth forms (1). In particular shrublands, which can be considered a mixture of several sclerophyllous shrubs and tree species, represent a Mediterranean type of vegetation rarely found in other ecosystems. The distribution of shrubs in an ecosystem is connected to complex ecological processes. Indeed, shrubs provide substrate, food and shelter for organisms, play an important role in the nutrient cycle providing organic matter input to soils and they protect watersheds from erosion. Moreover shrubs are very important for many associated species such as mammals, birds, invertebrates, other plants (that favour thanks to their role of nurse plants), and their distribution also influences the variety and abundance of mycorrhizal fungi, fundamentals in ecological terms and nutritional relationships (2). This study represents a review on the ecological role of some selected Mediterranean shrubby species. In particular the attention is focused on the following species: Amelanchier ovalis Medik, Astragalus nebrodensis (Guss.) Strobl., Crataegus laciniata Ucria, Lycium intricatum Boiss., Prunus spinosa L., and Viburnum tinus L. These species have been selected because they are representative of different Mediterranean vegetation types, distributed in relation to the bioclimate type and the altitude. In fact, various habitats have been considered in relation to these species, for example coastal and sub-coastal environments for Lycium intricatum, sclerophyllous woodlands for Viburnum tinus, mantle vegetation and pre-forest structures for Amelanchier ovalis, Prunus spinosa and Crataegus laciniata, orophilous thornycushions vegetation for the Sicilian endemic Astragalus nebrodensis. For each species the geographical distribution in the Mediterranean Basin has been investigated, considering its borders according to the limits of Mediterranean climate (3), and the definition of terrestrial ecoregions of the world (4). The species distribution as well as their taxonomy (especially in relation to the presence of subspecies in the Italian territory) have been analyzed thanks to a number of scientific publications and web-based sources (e.g. Euro + Med PlantBase, The International Plant Names Index, ILDIS- International Legume Database & Information Service, PAN-European Species directories Infrastructure, Portale della Flora d\u2019Italia, Flora of Greece Web, Anthos, African Plant Database, Silene-Flore database, etc.) at regional, national and international scale. Moreover for each species distribution maps have been prepared geo-referencing the species location data obtained from the labels of digital herbaria samples of the Mus\ue9um National d'Histoire Naturelle, MNHN \u2013 Paris, P; Naturhistorisches Museum Wien, W; Herbarium Mediterraneum Panormitanum, PAL; Herbarium Plant Biology Section, CAT; Botanischer Garten und Botanisches Museum Berlin-Dahlem, Zentraleinrichtung der Freien Universit\ue4t Berlin, B, and from the data portal Global Biodiversity Information Facility- GBIF (always in relation to herbarium samples), also in order to obtain historical distribution data. The ecological role of species in every ecosystem, but also of their successional dynamics and their response to global changes and disturbances was investigated making an analysis of the literature data and also in relation to some botanical aspects and functional traits, available for some of the studied species, from TRY Plant Trait Database (https://www.try-db.org) and form BROT 2.0 database, for example: Growth form, Leaf phenology, Spinescence, Flower color, Dispersal mode, Fruit type, Nutritional relationships (the analysis of these data is in progress especially with regard to mycorrhizal symbioses). This has allowed to clarify why shrubs are successful in many habitats and to evaluate the critical role that they play in Mediterranean ecosystems

Wood-decay fungi on trees of the city of Palermo (Sicily, Italy)

Nineteen taxa, belonging to 15 genera, included in 12 families of Basidiomycetes were observed on different living trees and stumps. Data on hosts and collection sites are reported together with notes on the causes of wood decay fungi attack and suggestions for solutions

An insight into the presence of lignicolous fungi in Sicily (southern Italy)

Thirty-six lignicolous fungal species (4 Ascomycota and 32 Basidiomycota), included in 16 families and 28 genera have been recorded in different natural and reforested areas of Sicily. Amylostereum laevigatum, Ceriporia excelsa, Phlebia lilascens, and Stereum insignitum represent new records for Sicily. Notes on some rare species are also here reported

Medicinal Mushrooms: Bioactive Compounds, Use, and Clinical Trials

Medicinal mushrooms have important health benefits and exhibit a broad spectrum of pharmacological activities, including antiallergic, antibacterial, antifungal, anti-inflammatory, antioxidative, antiviral, cytotoxic, immunomodulating, antidepressive, antihyperlipidemic, antidiabetic, digestive, hepatoprotective, neuroprotective, nephroprotective, osteoprotective, and hypotensive activities. The growing interest in mycotherapy requires a strong commitment from the scientific community to expand clinical trials and to propose supplements of safe origin and genetic purity. Bioactive compounds of selected medicinal mushrooms and their effects and mechanisms in in vitro and in vivo clinical studies are reported in this review. Besides, we analyzed the therapeutic use and pharmacological activities of mushrooms

First report of Diplodia africana on Grevillea robusta

Branch cankers and dieback were observed on silky-oak trees (Grevillea robusta) along some streets of Palermo (Sicily, Italy). Fungi isolated from symptomatic branches were identified as Diplodia africana and D. seriata by morphological charac-ters and phylogenetic analyses of combined ITS and translation elongation factor 1-α sequences. Pathogenicity was verified by inoculating twigs of 3-y-old silky-oak plants. This is the first report of D. africana on G. robusta and the first record of D. seriata on this host in the northern hemisphere

Trees and shrubs in the city of Bari (Italy)

The authors recorded in the city of Bari 221 specific and infraspecific taxa, used as onramentals, belonging to 126 genera of 64 families with a prevalence of exotic species over native ones. The study is preparatory to the redevelopment of urban green areas concerning the recent Municipal Regulation on green spaces in the city. The flora investigated is rich in elements native to Italy. Anyway, the most common species are commonly used as ornamental also in the other regions of Italy. Argania spinosa, Ceiba speciosa, Cyperus papyrus, Euphorbia ingens, Ficus rubiginosa, and Jacaranda mimosifolia are species less commonly used and noteworthy, because at their climatic limit

The Checklist of Sicilian Macrofungi: Second Edition

Approximately 30 years after the publication of the first Sicilian checklist of macrofungi,a new updated version is presented here. The census of macromycetes was carried out through periodic observations in different agricultural and forest ecosystems, in urban areas, in public and private gardens, and in botanical gardens. The 1919 infraspecific taxa included in 508 genera belonging to 152 families were collected in the Sicilian territory. Ectomycorrhizal fungi are the most represented ecological category, followed by saprotrophs on wood, saprotrophs on litter, and terricolous saprotrophs. The interest in this rich group of organisms is evidenced by the nutritional and therapeutic value of a high percentage of species. The actions linked to the National Recovery and Resilience Plan and The Network for the Study of Mycological Diversity will further increase the number of macrofungi for Sicily in the future

Forest nurseries and the National Recovery and Resilience Plan: the case of Sicily and Apulia (Italy)

In Italy, the National Recovery and Resilience Plan (NRRP) foresees the planting of ca. 6.6 million trees to establish urban and peri-urban forests in 14 metropolitan cities. This ambitious project requires a significant number of native trees and shrubs, currently unavailable in Italian public and private nurseries. This survey analyzes the state of forest nurseries in two administrative regions of southern Italy, i.e. the “Filici” forest nursery (province of Agrigento, Sicily) and the Gargano Mountain Reclamation Consortium (province of Foggia, Apulia), to evaluate the adequacy of Sicilian and Apulian forest nurseries as potential sources of plant material to meet the requirements of the NRRP. The census carried out at the “Filici” nursery revealed the presence of more than 22,000 seedlings in cultivation, comprising 55 species, 26 genera, and 26 families. The autochthonous species are 43 (78.2%), while the exotic ones are 12 (21.8%). The Gargano Mountain Reclamation Consortium forest nursery has 190,876 seedlings in cultivation. A total of 80 species are present, belonging to 59 genera and 32 families. Of these, 68 (85%) are native species, 12 (15%) are exotic. An analysis of the plant material being cultivated in these forest nurseries shows the presence of a share of autochthonous species of interest for urban reforestation initiatives, however these are still insufficient in number

Microbial Safety of Black Summer Truffle Collected from Sicily and Umbria Regions, Italy

Background: Tuber aestivum Vittad., known as black summer truffle, represents high-value food especially used as garnishment in nouvelle cuisine. The aim of this study was to investigate on the viable microbial populations associated with T. aestivum ascomata collected in different sites of Sicily and one locality of Umbria (Italy). Methods: The ripe ascomata of black summer truffles were collected from Central Italy. Cell densities of spoilage bacteria, fecal indicators, potential pathogens, yeasts, and molds were analyzed. Statistical analysis was conducted with XLSTAT software. Results: The microbiological counts of truffles ranged between 6.00 and 9.63 log Colony Forming Unit (CFU)/g for total mesophilic count and between 6.18 and 8.55 log CFU/g for total psychrotrophic count; pseudomonads were in the range 6.98-9.28 log CFU/g. Listeria spp. and coagulase-positive streptococci detected in no samples. Coagulasenegative streptococci were found in some samples with 2.11-4.76 log CFU/g levels. Yeasts and filamentous fungi were detected at consistent levels of 3.60-7.81 log CFU/g. Significant differences (p<0.01) were found between samples and also for all microbial groups. Conclusion: This study evidenced that the common brushing procedure applied for preparation of truffles is not sufficient to eliminate microbial risks for consumers. The application of an efficient decontamination treatment is strongly suggested before consumption of fresh truffles