7 research outputs found
Sequencing Analysis of Genetic Loci for Resistance for Late Leaf Spot and Rust in Peanut (Arachis hypogaea L.)
The aim of this study was to identify candidate resistance genes for late leaf spot (LLS) and rust diseases in peanut (Arachis hypogaea L.). We used a double-digest restriction-site associated DNA sequencing (ddRAD-Seq) technique based on next-generation sequencing (NGS) for genotyping analysis across the recombinant inbred lines (RILs) derived from a cross between a susceptible line, TAG 24, and a resistant line, GPBD 4. A total of 171 SNPs from the ddRAD-Seq together with 282 markers published in the previous studies were mapped on a genetic map covering 1510.1 cM. Subsequent quantitative trait locus (QTL) analysis revealed major genetic loci for LLS and rust resistance on chromosomes A02 and A03, respectively. Heterogeneous inbred family-derived near isogenic lines and the pedigree of the resistant gene donor, A. cardenasii Krapov. & W.C. Greg., including the resistant derivatives of ICGV 86855 and VG 9514 as well as GPBD 4, were employed for whole-genome resequencing analysis. The results indicated the QTL candidates for LLS and rust resistance were located in 1.4- and 2.7-Mb genome regions on A02 and A03, respectively. In these regions, four and six resistance-related genes with deleterious mutations were selected as candidates for LLS and rust resistance, respectively. These delimited genomic regions may be beneficial in breeding programs aimed at improving disease resistance and enhancing peanut productivity
Summary of number of loci common between genetic maps for different mapping populations.
<p>Summary of number of loci common between genetic maps for different mapping populations.</p
A microsatellite consensus genetic map comprising 897 marker loci based on 11 mapping populations.
<p>Markers are shown on <i>right</i> side of the LG while map distances are shown on the <i>left</i> side. Each LG has been divided into 203 BINs of 20 cM each. The homoeologous loci between the corresponding LGs in the reference consensus map are indicated in red colour.</p
Features of the component and reference consensus genetic maps.
<p>Features of the component and reference consensus genetic maps.</p
Source of marker data used for constructing the reference consensus genetic map.
<p>Source of marker data used for constructing the reference consensus genetic map.</p
Comparison between the LGs of the reference consensus map and the diploid AA and BB maps.
<p>The LGs of the reference consensus map are represented as a01 to a10 and b01 to b10. The LGs of AA map are named as Group 1 to Group 11 and for BB map as B1 to B10 respectively (published by Moretzsohn et al 2005, 2009). The AA map was represented by a red bar and the BB map with green colour. The common markers between corresponding LGs in the reference consensus map and AA map are indicated in red colour and pink colour with BB map.</p
Features of the reference consensus genetic map.
<p>Features of the reference consensus genetic map.</p