脂肪由来多系統前駆細胞他家移植による歯周組織再生能力の解析

要約のみTohoku University齋藤正寛課

A multiplex PCR for detection of haemolytic aeromonas hydrophila from vegetable sources in Karnataka, India

Aeromonas hydrophila and other aeromonads are ubiquitous organisms commonly found in majority of food matrices intended for human consumption. They cause diarrhea, septicemia and extra-intestinal infections in normal and immunocompromised patients. The aim of the present study, to develop a multiplex PCR based strategy for the detection of pathogenic Aeromonas spp. by targeting virulence-associated genes including aerolysin (aerA), cytotonic enterotoxins (alt), serine protease (Ahe 2) gene, along with a 16S rRNA gene specific to genus  Aeromonas. A competitive internal amplification control (IAC) was introduced into mPCR assay to negotiate false negative results during PCR reaction. The results showed that, developed method was useful for specific detection and differentiation of pathogenic and non pathogenic strains of Aeromonas spp. mPCR method was successfully evaluated onto several spiked as well as naturally contaminated food samples originated form Karnataka, India. The sensitivity of developed method was determined as 5x10-5 CFUml−1 from experimentally spiked food and water samples. To check the practical usefulness of developed mPCR method a total of 120 vegetable samples were evaluated. Out of 120 samples, eight samples were stayed as positive for A.  hydrophila contamination. Multiplex PCR results were well correlated with that off conventional culture based methods. In conclusion developed mPCR method could provide a powerful tool for detection and differentiation of pathogenic A. hydrophila from food and environmental samples. And also it could be a better alternative for time consuming conventional culture based methods for routine food analysis laboratories

Somatic embryogenesis and plant regeneration of Capsicum baccatum L.

AbstractA plant regeneration protocol via somatic embryogenesis was achieved in cotyledon and leaf explants of Capsicum baccatum, when cultured on MS medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D, 0.5–5.0mgl−1) in combination with Kinetin (Kn, 0.5mgl−1) and 3% sucrose. Various stages were observed during the development of somatic embryos, including globular, heart, and torpedo-stages. Torpedo stage embryos were separated from the explants and subcultured on medium supplemented with various concentrations of different plant growth regulators for maturation. Maximum percentage (55%) of somatic embryo germination and plantlet formation was found at 1.0mgl−1 BA. Finally, about 68% of plantlets were successfully established under field conditions. The regenerated plants were morphologically normal, fertile and able to set viable seeds

DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers

In the present study, genetic variability in 10 commercial chilli pepper varieties viz. Gemini, G-334, Agnirekha, Pusa jwala, Mangala, Black diamond, Sindura, Pusa jyothi, Badiga-2 and Teja (branch), cultivated in the local area of Andhra Pradesh State, India, using random amplified polymorphic DNA (RAPD) markers was examined. Out of thee RAPD markers used for screening 10 chilli pepper genotypes, DNA amplification was observed only with OPAB02; this primer produced monomorphic band in Gemini,G-334, Black diamond and Sindura and polymorphic bands in Agnirekha, Pusa jwala, Mangala, Pusa jyothi, Badiga-2 and Teja (Branch). Genetic variabilty in terms of DNA pattern produced in the above varieties could be used as a marker to distinguish between them. Dendrogram generated by OPAB02 primer showed that the 10 chilli pepper varieties could be grouped into four clusters. Average genetic similarity index revealed 100% similarity between varieties of first cluster, 50% similarity between varieties of second and fourth clusters and 32% genetic similarity between varieties of third cluster. UPGMA cluster analysis will be useful in chilli pepper breeding programmes and germplasm conservation. Since the commercial value of chilli pepper is based on pungency level, future studies are aimed at molecular marker based pungency phenotyping.Keywords: Capsicum annuum, Randomly Amplified Polymorphic DNA, Dendrogram, Polymerase Chain Reaction.African Journal of Biotechnology Vol. 12(30), pp. 4730-473

Alternating domains with uniaxial and biaxial magnetic anisotropy in epitaxial Fe films on BaTiO[sub 3]

We report on domain formation and magnetization reversal in epitaxial Fe films on ferroelectric BaTiO3 substrates with ferroelastica–c stripe domains. The Fe films exhibit biaxial magnetic anisotropy on top of c domains with out-of-plane polarization, whereas the in-plane lattice elongation of a domains induces uniaxial magnetoelasticanisotropy via inverse magnetostriction. The strong modulation of magnetic anisotropy symmetry results in full imprinting of the a–c domain pattern in the Fe films. Exchange and magnetostaticinteractions between neighboring magnetic stripes further influence magnetization reversal and pattern formation within the a and c domains.Peer reviewe