Gender differences in skin and core body temperature during exercise in a hot, humid environment.

Background. It is universally accepted that men and women regulate heat differently during exercise in hot, humid environments. Despite this common knowledge, little empirical data is available to explain how gender differences effect core/skin temperature changes. Further, the data that is available includes only limited measurement sites and/or time points. Methods. The purpose of this study was to measure the heat regulatory patterns for both men and women during 60-min of exercise in a hot, humid environment. Twenty aerobically fit subjects (10 men; 10 women) completed an acclimation session followed by a 60-min exercise protocol in an environmental chamber set at 39.9 ± 1.1 °C and 46.4 ± 4% relative humidity. Each exercise protocol consisted of four intervals of an 8-min walk (mean ± SD; men: 4.0 ± 0.3, women: 3.0 ± 0.2 mph) and 7-min light jog (men: 5.4 ± 0.5, women: 5.0 ± 0.3 mph). Subjects were confirmed to be euhydrated (urine specific gravity) prior to exercise and were encouraged to drink water to maintain hydration during exercise. Pre- and Post-exercise body weights did not differ, thus it appears hydration was maintained during exercise. Skin temperatures (iButton wireless loggers) were recorded every minute at seventeen sites (right and left: upper chest, mid-chest, abdomen, upper back, mid-back, lower back, upper arm, and lower arm, and back of the neck). In addition, core body temperature (rectal), rating of perceived exertion (RPE), and heart rate (wireless telemetry) were also recorded at the end of each interval of the protocol. Statistical analysis was carried out using a 2 (gender) x 60 (time) repeated measures ANOVA. Other variables were analyzed using a 2 (gender) x 8 (time) ANOVA with repeated measures on the 2nd factor. Any non-normally distributed data was log transformed. Significance was set at p\u3c0.05 and location of effects will be determined using individual t-tests with a Bonferroni correction for multiple comparisons. Results. Analysis revealed significantly higher skin temperatures in four locations on men compared to women: right upper back (p=0.048), right mid-back (p=0.001), right lower back (p=0.001), and left upper back (p\u3c0.001). Additionally, these changes were seen despite no significant differences between genders in core body temperature, RPE, and heart rate. This latter finding supports the conclusion that a similar degree of exercise, heat stress was applied to both men and women. Conclusions. We found gender differences at four different skin temperature locations. These changes might suggest that men retain more metabolic heat in various locations on the back when exercising in a hot, humid environment compared to women. Further research is needed to understand how these changes may affect post-exercise recovery return to baseline skin and core temperature values

Comparing Three Methods of Measuring Skin Temperature during Exercise in a Hot, Humid Environment

Exercise in a hot, humid environment substantially increases the physiological stress of exercise. Most of the techniques that are currently used to monitor changes in skin temperature have been in use for more than 20 years with little innovation. The purpose of the present study was two-fold: 1) to compare three techniques for measuring skin temperature (wired skin electrode, wireless temperature data logger, and thermal imaging) and 2) to compare and contrast these measures in men and women completing 45-min of cycling in a hot (39±2°C), humid (45±5% RH) environment. The CPHS committee approved all procedures described in this report and subjects gave written consent to participate. Men (N=14) and women (N=18) completed all study requirements out of 45 subjects that were enrolled. Following a baseline screening session that included a measurement of body composition (whole body DXA scan) and an aerobic fitness test (VO2peak), subjects were scheduled for an experimental exercise trial between 0500 and 0800. Subjects arrived to the laboratory and were tested for hydration using urine specific gravity and if dehydrated, they were provided water to drink before starting the exercise trial. Exercise consisted of 50-min of cycling. After 50-min, subjects were asked to continue cycling until their rectal core body temperature exceeded 39.3°C. The time they were able to exercise beyond 50-min was recorded and compared between individuals. Wired skin temperature was monitored using YSI400 banjo probes, wireless skin temperature was monitored using a data logging system (iButton), and thermal skin temperature was measured from images taken with a thermal camera (RAZR Max-IR). These skin measurements were made on the bicep and abdomen. All body temperatures were recorded at rest, every 10-min during exercise, and immediately following the end of exercise. Data was statistically analyzed using a 2 (gender: men & women) x sensor method (wired, wireless, and thermal) x time (0, 10, 20, 30, 40, 50, end) ANOVA with repeated measures on the 2nd and 3rd factors. Significance was set a

Behavior of Coupled Automata

We study the nature of statistical correlations that develop between systems of interacting self-organized critical automata (sandpiles). Numerical and analytical findings are presented describing the emergence of synchronization between sandpiles and the dependency of this synchronization on factors such as variations in coupling strength, toppling rule probabilities, symmetric versus asymmetric coupling rules, and numbers of sandpiles

Sex Differences in Change in Skin Temperature When Exercising in a Hot, Humid Environment

The risk for heat-related illness is increased when exercising in a hot, humid environment. In an effort to protect the athlete, body temperature is measured continuously while exercising in extreme environments. Currently, researchers and laboratory personnel employ the use of mean skin temperature to monitor athlete safety; however, this measurement fails to consider localized changes in temperature that may arise as a function of sex and exercise time. Therefore, the purpose of this study was to examine potential sex differences in the change in skin temperature at 17 different upper body locations while exercising in a hot, humid environment. Young men and women were recruited and completed a 60-min walk/jog interval protocol in a hot (34.1 ± 1 °C), humid (64 ± 8%) environment while skin temperature was continuously measured. To account for differences that may have arisen due to differing workloads between men and women, energy expenditure and metabolic heat production were calculated after the completion of exercise. Data was analyzed either a repeated-measures ANOVA (change in skin temperature) or t-test­ (energy expenditure and metabolic heat production). Location of interaction effects was determined using a Fisher’s Least Significant Difference test. Significance was set a p\u3c0.05 for all statistical testing. There was no difference between men and women in total energy expenditure; however, men were found to have a higher metabolic heat production. Women had a higher change in skin temperature at three locations on the back (left upper, right upper, and right mid-back). Conversely, there were no differences at any time point between men and women in the change in core temperature from baseline measurements. This study highlights the need to further investigate sex differences in cooling mechanisms while exercising in a hot, humid environment

Consumption of a high-fat meal increased monocyte adhesion molecule expression and oxLDL phagocytosis: implications for cardiovascular disease risk?

Macrophage-derived foam cells are the predominant component of arterial plaques in the early stages of atherosclerosis. The deposition of arterial plaques is effected by several factors that are influenced by a person’s daily nutritional habits. One factor that poses a major risk for plaque development is high levels of plasma LDL resulting from the consumption of a high-fat meal. In order to understand how an individuals’ diet effects arterial plaque deposition via the process of foam cell formation, we measured the acute response in circulating monocyte activity after consuming a high-fat meal. Samples were acquired on a FlowSight (EMD Millipore) equipped with 405, 488, 642, and 785 nm lasers. Samples were analyzed in IDEAS software to identify pro-inflammatory (CD14+/16+) and classic (CD14+/16-) monocytes. We measured monocyte concentration, adhesion molecule expression, scavenger R expression, and oxLDL phagocytosis for 5 h postprandial. We found that consuming a high-fat meal caused an increase in pro-inflammatory monocyte concentration, adhesion molecule expression, monocyte phagocytosis of oxLDL, and CD36 expression in pro-inflammatory monocytes. These results suggest that consuming a high-fat meal increases the potential of monocytes to become foam cells for at least 5 h postprandial

Using Dry Blood Spots to Evaluate Serum Cytokines and Chemokines in Humans via Multiplex Technology

Introduction: Dried Blood Spot (DBS) analysis has been used routinely since 1963 for the assessment of metabolic diseases in neonates; however, recent efforts have focused on the refinement and validation of DBS for other patient populations. The purpose of this study was to adapt existing DBS methods to analyze 38 serum cytokines/chemokines in human subjects. Validation will be completed by comparing DBS to serum for a given analyte. Methods: After providing informed consent, subjects (N=21) provided a finger-stick DBS or venous serum sample using standard technique. Subjects were apparently healthy, non-obese, and had no known disease. Finger-stick capillary blood samples were collected on Whatman 903 Protein Saver Card (Maidstone, U.K.) and frozen with desiccant in sealed bags prior to elution. DBS samples (2, 6 mm punches) were eluted and transferred through 96-well Multiscreen and Ultracel plates (EMD Millipore) using elution buffer (PBS with 0.5 M NaCL and 0.1% Tween-20) for 16-18 h in the refrigerator. The resultant DBS elute was resuspended in 90 mL of nuclease-free water. Serum samples were thawed overnight in the refrigerator to prevent protein loss. Prior to Milliplex analysis, protein and lipid content were analyzed using a IR-based spectrometer (EMD Millipore Direct Detect). DBS solutions were also analyzed for hemoglobin concentration. Samples were analyzed in pairs to determine the concentration 38 cytokines/chemokines using a Magnetic Multiplex Kit (Milliplex Map Kit High Sensitivity Human Cytokine; Billerica ,MA), A minimum of 50 beads of each targeted analyte were collected on a Luminex MagPix (Austin, TX) and analyzed using Milliplex Analyst Software. Bi-variate correlations were completed in SPSS to compare DBS with serum. Results: Of the 38 markers, 13 were measurable in both DBS and serum, and 3 of these were significantly correlated with each other: Eotaxin (.755), MDC (.446), IP-10 (.831). Based on a post-hoc sample size analysis we need to expand our sample size by approximately 40 subjects in order to establish significant correlations the other 10 DBS measurable cytokines/chemokines. Conclusion: Utilization of DBS may make it possible to obtain previously unobtainable blood samples in a field setting. Despite the potential for DBS all serum markers have not been validated in this sample source. An increase in validated biomarkers detectable in DBS could make the decision to utilize DBS over venipuncture easier. Now that we have identified changes in resting cytokines/chemokines, the next logical step is to evaluate the effect of exercise

Reduced Inflammatory and muscle damage biomarkers following oral supplementation with bioavailable curcumin

This article seeks to determine the effects of oral curcumin supplementation on muscle and activities of daily living soreness, creatine kinase, and inflammatory cytokines following exercise-induced muscle damage

Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise

Exercise and physical labor in extreme environmental conditions causes transient decreases in immune cell and cytokine concentrations, likely increasing the susceptibility to opportunistic infection. Baker's yeast beta glucan (BYBG) has been previously demonstrated to be an effective countermeasure in athletes, but its effectiveness in individuals of average fitness under similar physical stress is unknown. The purpose of this study was to determine if 10 days of oral supplementation with BYBG could modify previously observed suppression of monocytes, T cells, circulating and whole blood LPS-stimulated cytokines due to strenuous exercise. Venous blood samples were collected from 109 healthy volunteers prior to, immediately after, 2 and 4 h post-exercise. Monocyte and T cell concentration, cell-surface receptor expression and serum and LPS-stimulated cytokines were assessed. BYBG significantly (P < 0.05) altered total and classic monocyte concentration and expression of CD38, CD80, CD86, TLR2, and TLR4 on monocyte subsets. BYBG also significantly increased CD4+ and CD8+ T cell concentration and the exercise response of CCR7+/CD45RA- central memory (TCM) cells. Likewise, BYBG significantly (P < 0.05) altered serum IFN-γ and IL-2, and LPS-stimulated IFN-γ, IL-2, IL-4, and IL-7. Taken together these data support the hypothesis that oral BYBG supplementation modulates the expected exercise response for individuals of average fitness. This may result in a decrease in susceptibility to opportunistic infections after strenuous exercise

Comparison of techniques for the measurement of skin temperature during exercise in a hot, humid environment

Exercising or working in a hot, humid environment can results in the onset of heat-related illness when an individual’s temperature is not carefully monitored. The purpose of the present study was to compare three techniques (data loggers, thermal imaging, and wired electrodes) for the measurement of peripheral  (bicep) and central (abdominal) skin temperature. Young men and women (N=30) were recruited to complete the present study. The three skin temperature measurements were made at 0 and every 10-min during 40-min (60% VO2max) of cycling in a hot (39±2°C), humid (45±5% RH) environment. Data was statistically analyzed using the Bland-Altman method and correlation analysis. For abdominal skin temperature, the Bland-Altman limits of agreement indicated that data loggers (1.5) were a better index of wired than was thermal imaging (3.5), For the bicep skin temperature the limits of agreement was similar between data loggers (1.9) and thermal (1.9), suggesting the both were suitable measurements. We also found that when skin temperature exceeded 35ºC, we observed progressively better prediction between data loggers, thermal imaging, and wired skin sensors. This report describes the potential for the use of data loggers and thermal imaging to be used as alternative measures of skin temperature in exercising, human subjects

Non-invasive Measures of Core Temperature versus Ingestible Thermistor during Exercise in the Heat

This article discusses a study to cross-validate temporal, two tympanic devices, and oral devices compared to an ingestible thermistor during exercise in a hot, humid environment