Histopathological examination of endo-ovarian tissue biopsy.

<p>a) polymorphonuclear cells were observed in endometrial tissue biopsies; b) Severe lesions with granulomatous filled with beaded and malignant lymphoid cells were observed in endo-ovarian tissue biopsies; c) appearance of spindle-cells with superficial strips of positive endometrial glands and stroma; d) lymphocytic infiltrations and initial stages of granulomatous were observed. <b>Note</b>: The microscopic studies were carried out with tissue biopsies and aspirated fluids containing tissue pieces. Thereafter, the slides were viewed under bright field (40X), Inverted microscope. The contrasts of the photographs are changed to improve the visibility.</p

Statistical association of laparoscopic findings among infertile women with FGTB and HCW without TB.

<p><b>Note</b>: Statistical analysis showed 0 cells (0.0%) have an expected count less than 5 for all methods. The MEC for different methods were different, as the MEC for “a” is 36.73; “b” is 18.55; “c” is 30.18; “d” is 42.91; “e” is 15.64; “f” is 12.00; “g” is 6.55; “h” is 23.64; “i” is 18.18; “j” is 22.18 and “k” is 10.91; “l” denotes the use of a binomial distribution. Degree of freedom (df) was one (1) for all calculations. Data were considered statistically significant if <i>p</i>-value was less than 0.05.</p><p>Statistical association of laparoscopic findings among infertile women with FGTB and HCW without TB.</p

Diagnosis and statistical significance of FGTB infection among infertile women and HCW without TB.

<p><b>Note</b>: Statistical analysis showed 0 cells (0.0%) have an expected count less than 5 for all methods. Variation was observed for different methods in terms of the minimum expected counts, i.e. MEC for “a” is 33.45; “b” is 15.64; “c” is 30.55; “d” is 29.09 and “e” is 36.36. The “f” denotes the use of a binomial distribution. Degree of freedom (df) was one (1) for all calculations. Data were considered statistically significant if <i>p</i>-value was less than 0.05. Molecular analysis includes polymerase chain reaction (PCR) for 19kDa (131bp), 19kDa antigen gene; TRC4 (173bp), TRC4 element. AFB +Ve, AFB positive.</p><p>Diagnosis and statistical significance of FGTB infection among infertile women and HCW without TB.</p

Arithmetical association of menstrual problems among infertile women with FGTB and HCW without TB.

<p><b>Note</b>: Statistical analysis showed 0 cells (0.0%) have an expected count less than 5 among all methods, except for “f” 1 cell (25.0%) has expected count less than 5 and the MEC is 3.27. The MECs for different methods were different, as the MEC for “b” is 30.55; “c” is 9.09; “d” is 8.00; “e” is 5.82; “g” is 5.45 and “h” is 21.45. “a” denotes the use of a binomial distribution. Degree of freedom (df) was 1(one) for all calculations. Data were considered statistically significant if <i>p</i>-value was less than 0.05.</p><p>Arithmetical association of menstrual problems among infertile women with FGTB and HCW without TB.</p

Amplification refractory mutation system-multi gene/ multi primer polymerase chain reaction (ARMS-MG/MP-PCR) method.

<p>ARMS-MG/MP-PCR method genotyping assay for detection of G to A nucleotide substitution for TLR2 and T to A substitution for IFN-γ gene. Results for the three possible genotypes (GG, AG and AA) for TLR2 and IFN-γ gene (TT, TA, AA) are shown above the PCR products. Three percent agarose gel electrophoresis was carried out with ARMS-MG/MP-PCR products: Lanes 51a to 55g were loaded with ARMS-MG/MP-PCR products of female genital tuberculosis (FGTB) patients. Lanes C80a to C81g was loaded with PCR products of healthy control women (HCW) without tuberculosis (TB). Lane 100 bp Mrkr was loaded with 100 base pair (bp) molecular weight marker (the 100bp size of product starts from the bottom side of the gel and ends with 1000 bp of product on the top/upper side of the gel). The upper bands symbolize the 470 bp internal control PCR fragment, the 328 bp bands represent the ‘<b>A</b>’ (lane 51a) and ‘<b>G</b>’ (lane 55g) alleles of TLR2 (Arg753Gln) gene. The 261 bp bands represent the ‘<b>A</b>’ (lane 51a) and ‘<b>T</b>’ (lane 55g) alleles of IFN-γ (+874) gene, and lower bands stands for the human β-globin gene (110 bp internal control). Primer dimers were also noted at the bottom, during the end of the sample run.</p

Association of infertility and Mantoux test among infertile women with female genital tuberculosis (FGTB) and healthy control women (HCW) without TB.

<p>Association of infertility and Mantoux test among infertile women with female genital tuberculosis (FGTB) and healthy control women (HCW) without TB.</p

Z-N staining of endo-ovarian tissue biopsy and cultures in the detection of AFB.

<p>a) Red/ pink colour rod like beaded structures were observed in the tissue biopsy; b) Red/ pink colour rod like structures were observed on pale blue background in the cultures.</p

Association of demographic findings among infertile women with female genital tuberculosis (FGTB) and healthy control women (HCW) without tuberculosis (TB).

<p><b>Note</b>: Data was represented as mean± standard deviation (SD). Data were considered statistically significant if <i>p</i>-value was less than 0.05. The degree of freedom (df) was one (1) for all calculations. kg: kilogram. m: meters. N: number of patients. ANOVA: analysis of variance.</p><p>Association of demographic findings among infertile women with female genital tuberculosis (FGTB) and healthy control women (HCW) without tuberculosis (TB).</p

Comparative Time to Detection (TTD) of culture-positive specimens on solid (LJ) medium (grey bar) or with automated liquid (BacT/alert) (white bars) medium in active pulmonary and extrapulmonary TB patients according to their HIV status.

<p>TTD in days: median (IQR).</p

Analysis of combination of the tests evaluated over 53 active pulmonary TB patients and 82 HCA being concomitantly tested and stratified by HIV status.

<p>Footnotes: HIV: human immunodeficiency virus; TB: tuberculosis; TST: Tuberculin skin test; HCA: healthy community adults; QFT-GIT: QuantiFERON® Gold in Tube (indeterminate results included as negative results); SM: smear microscopy.</p>*<p>X square test was used for statistical analysis.</p