Case Report: Congenital tuberculosis in an aborted dromedary camel fetus

Tuberculosis (TB) is a serious public health problem worldwide, especially in tropical developing countries. Nevertheless, reports on congenital TB in humans and animals are extremely rare. In this study, abortion was reported in an 8-year-old she-camel at the 9th month of gestation. The she-camel appeared healthy in clinical examination, had a good body condition score, normal appetite, and had no signs of respiratory disease and fever. The expelled placenta was dark red-colored, thickened, and edematous with multifocal to coalescing ecchymotic hemorrhages on the allantoic surface. The striking finding was multiple, white-yellow, solid nodular lesions in the fetal lung, the pleura, and the liver. On histopathology, typical granulomatous lesions were detected in the lung and the liver characterized by caseous necrosis surrounded by lymphocyte and macrophage infiltration and concentric layers of fibrosis. The Ziehl-Neelsen staining detected scarce acid-fast bacilli in lung and liver tissues. The DNA extracted from tubercular lesions from the lung and liver showed amplification of the IS6110 region of the Mycobacterium tuberculosis complex by PCR. The sequencing and phylogenetic analysis revealed a close association of these sequences with Mycobacterium tuberculosis. The she-camel was detected positive for a single intradermal tuberculin test performed 24 h after abortion. This is the first report on congenital TB caused by M. tuberculosis in a dromedary camel fetus with a possible vertical transmission

Marine Fungi: A Source of Potential Anticancer Compounds

Metabolites from marine fungi have hogged the limelight in drug discovery because of their promise as therapeutic agents. A number of metabolites related to marine fungi have been discovered from various sources which are known to possess a range of activities as antibacterial, antiviral and anticancer agents. Although, over a thousand marine fungi based metabolites have already been reported, none of them have reached the market yet which could partly be related to non-comprehensive screening approaches and lack of sustained lead optimization. The origin of these marine fungal metabolites is varied as their habitats have been reported from various sources such as sponge, algae, mangrove derived fungi, and fungi from bottom sediments. The importance of these natural compounds is based on their cytotoxicity and related activities that emanate from the diversity in their chemical structures and functional groups present on them. This review covers the majority of anticancer compounds isolated from marine fungi during 2012–2016 against specific cancer cell lines

Effect of tillage and crop rotations on pore size distribution and soil hydraulic conductivity in sandy clay loam soil of the Indian Himalayas

Metadata onlyTillage management can affect crop growth by altering the pore size distribution, pore geometry and hydraulic properties of soil. In the present communication, the effect of different tillage management viz., conventional tillage (CT), minimum tillage (MT) and zero-tillage (ZT) and different crop rotations viz. [(soybean–wheat (S–W), soybean–lentil (S–L) and soybean–pea (S–P)] on pore size distribution and soil hydraulic conductivities [saturated hydraulic conductivity (Ksat) and unsaturated hydraulic conductivity {k(h)}] of a sandy clay loam soil was studied after 4 years prior to the experiment. Soil cores were collected after 4 year of the experiment at an interval of 75 mm up to 300 mm soil depth for measuring soil bulk density, soil water retention constant (b), pore size distribution, Ksat and k(h). Nine pressure levels (from 2 to 1500 kPa) were used to calculate pore size distribution and k(h). It was observed that b values at all the studied soil depths were higher under ZT than those observed under CT irrespective of the crop rotations. The values of soil bulk density observed under ZT were higher in 0–75 mm soil depth in all the crop rotations. But, among the crop rotations, soils under S–P and S–L rotations showed relatively lower bulk density values than S–W rotation. Average values of the volume fraction of total porosity with pores 150 μm in diameter (pores draining freely with gravity) were 0.124, 0.096 and 0.095 m3 m−3 under CT, MT and ZT; and 0.110, 0.104 and 0.101 m3 m−3 under S–W, S–L and S–P, respectively. Saturated hydraulic conductivity values in all the studied soil depths were significantly greater under ZT than those under CT (range from 300 to 344 mm day−1). The observed k(h) values at 0–75 mm soil depth under ZT were significantly higher than those computed under CT at all the suction levels, except at −10, −100 and −400 kPa suction. Among the crop rotations, S–P rotation recorded significantly higher k(h) values than those under S–W and S–L rotations up to −40 kPa suction. The interaction effects of tillage and crop rotations affecting the k(h) values were found significant at all the soil water suctions. Both S–L and S–P rotations resulted in better soil water retention and transmission properties under ZT

Carbon sequestration and relationship between carbon addition and storage under rainfed soybean–wheat rotation in a sandy loam soil of the Indian Himalayas

Metadata onlySoil organic matter (SOM) contributes to the productivity and physical properties of soils. Although crop productivity is sustained mainly through the application of organic manure in the Indian Himalayas, no information is available on the effects of long-term manure addition along with mineral fertilizers on C sequestration and the contribution of total C input towards soil organic C (SOC) storage. We analyzed results of a long-term experiment, initiated in 1973 on a sandy loam soil under rainfed conditions to determine the influence of different combinations of NPK fertilizer and fertilizer + farmyard manure (FYM) at 10 Mg ha−1 on SOC content and its changes in the 0–45 cm soil depth. Concentration of SOC increased 40 and 70% in the NPK + FYM-treated plots as compared to NPK (43.1 Mg C ha−1) and unfertilized control plots (35.5 Mg C ha−1), respectively. Average annual contribution of C input from soybean (Glycine max (L.) Merr.) was 29% and that from wheat (Triticum aestivum L. Emend. Flori and Paol) was 24% of the harvestable above-ground biomass yield. Annual gross C input and annual rate of total SOC enrichment were 4852 and 900 kg C ha−1, respectively, for the plots under NPK + FYM. It was estimated that 19% of the gross C input contributed towards the increase in SOC content. C loss from native SOM during 30 years averaged 61 kg C ha−1 yr−1. The estimated quantity of biomass C required to maintain equilibrium SOM content was 321 kg ha−1 yr−1. The total annual C input by the soybean–wheat rotation in the plots under unfertilized control was 890 kg ha−1 yr−1. Thus, increase in SOC concentration under long-term (30 years) rainfed soybean–wheat cropping was due to the fact that annual C input by the system was higher than the required amount to maintaining equilibrium SOM content

Potassium balance as influenced by farmyard manure application under continuous soybean–wheat cropping system in a Typic Haplaquept

Metadata onlyThe effect of 30 years of continuous cropping, fertilization and manuring on the potassium (K) balances, the soil K pools and the nonexchangeable K release in a Typic Haplaquept soil from Almora, India under a rainfed soybean–wheat cropping system were investigated. The apparent K balance was measured as the difference between the total K added and that removed by the crops. The results showed that the total removal of K by the crops exceeded the amount of total K applied to the soil in all the treatments showing a net negative K balance. This ranged from 3.7 in the plots under NK to 81.7 kg ha−1 year−1 in the N+FYM treated plots. Continuous annual application of recommended doses of NPK+10 t FYM (NPK+FYM) to soybean resulted in an accumulation (+56 kg K ha−1) of exchangeable K (1 N NH4OAc extractable K) in the 0–45 cm soil depth over the study period, despite the highest average annual uptake of K by the system (150.8 kg ha−1 year−1). However, there was a net depletion of exchangeable K (−80 kg K ha−1) in that soil depth under the NPK treated plots. The results also revealed that the content of non-exchangeable K decreased substantially from 3482 kg ha−1 to 2677 and 2896 kg ha−1 in the 0–45 cm soil layer after 30 years of cropping in the plots under NPK+FYM and NPK treatments, respectively. There was a significant decline in total soil K with the removal of non-exchangeable soil K in the surface (0–15 cm) soil layer (R2=0.526,Pb0.001, n=36). Thus, long-term application of non-revised recommended fertilizer rates may threaten sustainability of the rainfed continuous soybean–wheat system

Pathways to sustainable waste management in Indian Smart Cities

To develop intelligent and sustainable cities, the government of India launched initiatives like Smart Cities and the Clean Development Mission. Solid waste management (SWM) has become one of the primarily focused areas of such initiatives. The changing dynamics of urban waste characteristics due to unplanned urbanization and rapid growth in urban populations (due to migration) has been a significant concern for local authorities in urban areas to develop an effective SWM plan. The development of a long-term SWM plan along the line of the government initiatives' objectives requires understanding the waste quantities, characteristics, and existing waste management practices. This paper presents an overview of existing waste management activities, financial and institutional demographics in six selected Indian Smart Cities, and the field studies to assess SWM systems as per the waste management rules. The study involved a participatory approach to collect data from local bodies and stakeholders. Indicative strategies for the development of waste management systems were formulated based on the outputs of this analysis. Discussion on pathways includes waste characterization, funding sources, data technologies, and service level benchmarking to plan an integrated solid waste management system for a Smart City. The outcomes of this study shall equip local authorities in designing waste systems to accelerate the transition towards innovative and sustainable waste systems

Sustainable solid waste management challenges in hill cities of developing Countries: Insights from eastern Himalayan smart cities of Sikkim, India

Rapid urbanization and the growing tourism industry in the Himalayan region have raised significant concerns about the sustainable handling and management of solid waste in the area. This concern is particularly pronounced in the unique and vulnerable ecosystem of cities nestled in the eastern Indian Himalayas, where geography, ecology, and socio-economic factors converge to create distinctive challenges. This study undertakes a thorough investigation of solid waste management practices and challenges in two smart cities nestled in the eastern Himalayan region of India, specifically Gangtok and Namchi. The primary objective was to unveil the complex challenges confronting these cities by conducting an in-depth exploration of waste management practices. This comprehensive investigation was achieved through the collection of samples, conducting surveys within each ward of the cities, and performing physicochemical characterization analyses. The study also proposes a solution-oriented approach leveraging life cycle analysis. Despite the Himalayan backdrop, waste generation varies significantly, with Gangtok producing substantially more waste (50 tons per day (TPD)) compared to Namchi (4.6 TPD). Both cities excel in waste collection, but Gangtok leads in proactive treatment, with 22% (11 TPD) undergoing composting and recycling. However, shared challenges at riverside disposal sites, including overfilled dumpsites and leachate contamination, pose potential environmental hazards amid the Himalayan allure. Organic waste predominates at 40.9% and 40.01% in both cities, indicating composting potential. Plastic closely at 22.25% (Namchi) and 22.65% (Gangtok) was way above the global (12 %) and national (8.4 %) average respectively, emphasizing the urgency of plastic waste reduction strategy. The 16.95% cardboard in Gangtok highlights potential commerce-related waste. The study also highlights the importance of comprehensive policies for informal sector well-being. The lifecycle assessment of Namchi and Gangtok reveals significant methane emissions (99%) from waste anaerobic decomposition potentially due to poor source segregation. Implementing a futuristic waste strategy reduces emissions by 38% in Namchi and 40% in Gangtok, through biogas utilization and renewable energy adoption. The findings advocate for citizen engagement, improved collection, targeted campaigns, and circular economy principles. To ensure sustainability and resilience in waste management within mountain regions, tailored strategies such as material recycling facilities, community engagement initiatives, and landfill diversion mandates should integrate ecological, socioeconomic, and logistical considerations, fostering awareness and participation among communities

Emerging Role of Autophagy in Governing Cellular Dormancy, Metabolic Functions, and Therapeutic Responses of Cancer Stem Cells

Tumors are composed of heterogeneous populations of dysregulated cells that grow in specialized niches that support their growth and maintain their properties. Tumor heterogeneity and metastasis are among the major hindrances that exist while treating cancer patients, leading to poor clinical outcomes. Although the factors that determine tumor complexity remain largely unknown, several genotypic and phenotypic changes, including DNA mutations and metabolic reprograming provide cancer cells with a survival advantage over host cells and resistance to therapeutics. Furthermore, the presence of a specific population of cells within the tumor mass, commonly known as cancer stem cells (CSCs), is thought to initiate tumor formation, maintenance, resistance, and recurrence. Therefore, these CSCs have been investigated in detail recently as potential targets to treat cancer and prevent recurrence. Understanding the molecular mechanisms involved in CSC proliferation, self-renewal, and dormancy may provide important clues for developing effective therapeutic strategies. Autophagy, a catabolic process, has long been recognized to regulate various physiological and pathological processes. In addition to regulating cancer cells, recent studies have identified a critical role for autophagy in regulating CSC functions. Autophagy is activated under various adverse conditions and promotes cellular maintenance, survival, and even cell death. Thus, it is intriguing to address whether autophagy promotes or inhibits CSC functions and whether autophagy modulation can be used to regulate CSC functions, either alone or in combination. This review describes the roles of autophagy in the regulation of metabolic functions, proliferation and quiescence of CSCs, and its role during therapeutic stress. The review further highlights the autophagy-associated pathways that could be used to regulate CSCs. Overall, the present review will help to rationalize various translational approaches that involve autophagy-mediated modulation of CSCs in controlling cancer progression, metastasis, and recurrence

Conventional and Zero Tillage with Residue Management in Rice–Wheat System in the Indo-Gangetic Plains: Impact on Thermal Sensitivity of Soil Organic Carbon Respiration and Enzyme Activity

The impact of global warming on soil carbon (C) mineralization from bulk and aggregated soil in conservation agriculture (CA) is noteworthy to predict the future of C cycle. Therefore, sensitivity of soil C mineralization to temperature was studied from 18 years of a CA experiment under rice–wheat cropping system in the Indo-Gangetic Plains (IGP). The experiment comprised of three tillage systems: zero tillage (ZT), conventional tillage (CT), and strip tillage (ST), each with three levels of residue management: residue removal (NR), residue burning (RB), and residue retention (R). Cumulative carbon mineralization (Ct) in the 0–5 cm soil depth was significantly higher in CT with added residues (CT-R) and ZT with added residues (ZT-R) compared with the CT without residues (CT-NR). It resulted in higher CO2 evolution in CT-R and ZT-R. The plots, having crop residue in both CT and ZT system, had higher (p < 0.05) Van’t-Hoff factor (Q10) and activation energy (Ea) than the residue burning. Notably, micro-aggregates had significantly higher Ea than bulk soil (~14%) and macro-aggregates (~40%). Aggregate-associated C content was higher in ZT compared with CT (p < 0.05). Conventional tillage with residue burning had a reduced glomalin content and β-D-glucosidase activity than that of ZT-R. The ZT-R improved the aggregate-associated C that could sustain the soil biological diversity in the long-run possibly due to higher physical, chemical, and matrix-mediated protection of SOC. Thus, it is advisable to maintain the crop residues on the soil surface in ZT condition (~CA) to cut back on valuable C from soils under IGP and similar agro-ecologies

Long-term yield trend and sustainability of rainfed soybean–wheatsystem through farmyard manure application in a sandy loam soil of the Indian Himalayas

A long-term (30 years) soybean–wheat experiment was conducted at Hawalbagh, Almora, India to study the effects of organic and inorganic sources of nutrients on grain yield trends of rainfed soybean (Glycine max)–wheat (Triticum aestivum) system and nutrient status (soil C, N, P and K) in a sandy loam soil (Typic Haplaquept). The unfertilized plot supported 0.56 Mg ha−1 of soybean yield and 0.71 Mg ha−1 of wheat yield (average yield of 30 years). Soybean responded to inorganic NPK application and the yield increased significantly to 0.87 Mg ha−1 with NPK. Maximum yields of soybean (2.84 Mg ha−1) and residual wheat (1.88 Mg ha−1) were obtained in the plots under NPK + farmyard manure (FYM) treatment, which were significantly higher than yields observed under other treatments. Soybean yields in the plots under the unfertilized and the inorganic fertilizer treatments decreased with time, whereas yields increased significantly in the plots under N + FYM and NPK + FYM treatments. At the end of 30 years, total soil organic C (SOC) and total N concentrations increased in all the treatments. Soils under NPK + FYM-treated plots contained higher SOC and total N by 89 and 58% in the 0–45 cm soil layer, respectively, over that of the initial status. Hence, the decline in yields might be due to decline in available P and K status of soil. Combined use of NPK and FYM increased SOC, oxidizable SOC, total N, total P, Olsen P, and ammonium acetate exchangeable K by 37.8, 42.0, 20.8, 30.2, 25.0, and 52.7%, respectively, at 0–45 cm soil layer compared to application of NPK through inorganic fertilizers. However,the soil profiles under all the treatments had a net loss of nonexchangeable K, ranging from 172 kg ha−1 under treatment NK to a maximum of 960 kg ha−1 under NPK + FYM after 30 years of cropping. Depletion of available P and K might have contributed to the soybean yield decline in treatments where manure was not applied. The study also showed that although the combined NPK and FYM application sustained long-term productivity of the soybean– wheat system, increased K input is required to maintain soil nonexchangeable K level