Alteration of Lysosomal Membrane and Enzymes Induced by Peptidoglycan Monomer (PGM)

The inñuence of single (4 mg/mouse) and multiple (1 mg/mouse/day for 5 consecutive days) injections of PGM on some hepatic enzymes, lipid peroxide generation in serum and liver and hepatic lysosomal membrane permeability was investigated. The studies performed showed that a single injection of PGM in vivo changed temporarily the permeability of lysosomal membranes, lipid peroxidation products and, when administered in vitro, modulated superoxide anion production and did not affect the activity of lysosomal membrane enzymes. Multiple injections of PGM caused no significant changes in the examined parameters. Although the metabolic changes were time-limited and, from the toxicological point of view, provoked transient effects, the results obtained may be of importance when using PGM in combined chemo-immunotherapy

Activities of Serum Esterases in Patients with Hyperlipidaemia

Esterases were studied in serum samples from 141 patients with hyperlipidaemia and 27 patients with hyperlipidaemia and non-in- sulin-dependent diabetes mellitus. The control group consisted of 322 non-diseased individuals. Paraoxon, beta-naphthylacetate and phenylacetate were used as substrates. Enzyme activities were measured in the absence and presence of EDTA in order to differentiate the EDTA-sensitive (y-sen) from the EDTA-insensitive Ivins) enzymes. Activity ranges for a given enzyme and substrate grossly overlapped between all the studied groups. However, sera from the control group had significantly higher median v-sen and y-ins activities for paraoxon, and higher y-sen and y-ins activities for PA, than the two groups of patients. HDL-cholesterol, total cholesterol, triglycerides and lipidograms were determined in serum samples from the two groups of patients. Serum samples with type V lipidogram had the lowest y-sen and y-ins activities for paraoxon, the lowest HDL-cholesterol and the highest triglyceride concentrations

Alteration of Lysosomal Membrane and Enzymes Induced by Peptidoglycan Monomer (PGM)

The inñuence of single (4 mg/mouse) and multiple (1 mg/mouse/day for 5 consecutive days) injections of PGM on some hepatic enzymes, lipid peroxide generation in serum and liver and hepatic lysosomal membrane permeability was investigated. The studies performed showed that a single injection of PGM in vivo changed temporarily the permeability of lysosomal membranes, lipid peroxidation products and, when administered in vitro, modulated superoxide anion production and did not affect the activity of lysosomal membrane enzymes. Multiple injections of PGM caused no significant changes in the examined parameters. Although the metabolic changes were time-limited and, from the toxicological point of view, provoked transient effects, the results obtained may be of importance when using PGM in combined chemo-immunotherapy