Media 1: Multispectral opto-acoustic tomography of exercised muscle oxygenation

Originally published in Optics Letters on 01 April 2015 (ol-40-7-1496

Media 2: Ultra-wideband three-dimensional optoacoustic tomography

Originally published in Optics Letters on 15 November 2013 (ol-38-22-4671

Media 2: Multispectral opto-acoustic tomography of exercised muscle oxygenation

Originally published in Optics Letters on 01 April 2015 (ol-40-7-1496

Media 1: Ultra-wideband three-dimensional optoacoustic tomography

Originally published in Optics Letters on 15 November 2013 (ol-38-22-4671

Supplementary document for Using reflectometry to minimize the dependence of fluorescence intensity on optical absorption and scattering - 6605127.pdf

Supplementary Informatio

Media 1: Real-time handheld multispectral optoacoustic imaging

Originally published in Optics Letters on 01 May 2013 (ol-38-9-1404

Media 2: Fast scanning coaxial optoacoustic microscopy

Originally published in Biomedical Optics Express on 01 July 2012 (boe-3-7-1724

Liver and gallbladder uptake of ICG.

<p>a) Optoacoustic images through the liver. Grayscale image (left) showing anatomy and ROIs for liver (red) and gallbladder (yellow) analysis. b) FCSI image: fluorescence from ICG overlaid in green on color photograph of cryosection of a mouse sacrificed 10 minutes after injection, showing signal in the liver and gallbladder c) Plot of the signal increase in the liver ROI at 800 nm during single wavelength imaging of the ICG injection. Oscillations are mainly due to breathing motion. The scale is normalized to the maximum value. d) Specific (unmixed) signal from ICG after injection in the liver (black) and gallbladder (blue) ROIs. Each curve is normalized to its own maximum value.</p

Whole-Cell Photoacoustic Sensor Based on Pigment Relocalization

Photoacoustic (optoacoustic) imaging can extract molecular information with deeper tissue penetration than possible by fluorescence microscopy techniques. However, there is currently still a lack of robust genetically controlled contrast agents and molecular sensors that can dynamically detect biological analytes of interest with photoacoustics. In a biomimetic approach, we took inspiration from cuttlefish who can change their color by relocalizing pigment-filled organelles in so-called chromatophore cells under neurohumoral control. Analogously, we tested the use of melanophore cells from Xenopus laevis, containing compartments (melanosomes) filled with strongly absorbing melanin, as whole-cell sensors for optoacoustic imaging. Our results show that pigment relocalization in these cells, which is dependent on binding of a ligand of interest to a specific G protein-coupled receptor (GPCR), can be monitored in vitro and in vivo using photoacoustic mesoscopy. In addition to changes in the photoacoustic signal amplitudes, we could furthermore detect the melanosome aggregation process by a change in the frequency content of the photoacoustic signals. Using bioinspired engineering, we thus introduce a photoacoustic pigment relocalization sensor (PaPiReS) for molecular photoacoustic imaging of GPCR-mediated signaling molecules

Validation of tomographic reconstructions.

<p>Comparison between OPT reconstructions and histological sections of molecular probe activity distribution (Prosense-680) in the inflamed heart after MI. Comparisons between reconstructions obtained with fluorescence OPT (A), Bornnormalized OPT (B), and the corresponding histological section (C). Born normalization preserves the molecular distributions in the reconstructed fluorescence channels. This is particularly evident for the papillary muscles located deep within the left ventricle, which appear less fluorescent without normalization, and the epicardium, which always shows bright fluorescent signal in absence of normalization. Born-normalized OPT reconstructions were obtained on the whole heart. The histological section (500 µm) belongs to the same specimen.</p