27 research outputs found
THE ISSUE OF PREGNANCY AND DELIVERY WITH PRENATAL INFECTION OF THE FETUS, HEALTH STATUS OF BABIES ON THE FIRST YEARS OF LIFE
Pregnancy and delivery of 27 women with prenatal infection of the fetus, neonatal period of the babies with prenatal pneumonia, health status of up to 2 children were researched in retrospect. Women with inflammation of the genitals (78,6%) and extragenital pathology of the inflammatory genesis (36,3%) had prenatal infection of the fetus. In the early neonatal period every baby had cerebral ischemia, every third child had haemorrhages in different cerebral structures. All babies have been transferred to children's hospital for treatment where the rehabilitation was carried out during 1-2,5 months. Infantile cerebral paralysis, hydrocephaly, epilepsy, epileptoid attacks were detected in 8 babies on the first year of life (29,6%). 5 babies (18,5%) had atrophy of discs of optic nerves, retinal angiopathy. All babies with local pathology of the central nervous system were premature born on the 26-32 week. Majority has herniae of different localization (55,5%), congenital dysplasia of the hip joint (74%), 26 babies (96,2%) were on the books because of anaemiae, pneumoniae, bronchites, pyelonephrites, septicaemiae. Using the received data we can draw a conclusion: in order to reduce perinatal complications in the maternal organism, in the organism of newborn baby and baby on the first year of life it is necessary to sanify the birth canal, to do preclinical diagnostics and preventive therapy of the prematurely born, gestosis, fetoplacental insufficiency
The HITRAN2020 Molecular Spectroscopic Database
The HITRAN database is a compilation of molecular spectroscopic parameters. It was established in the early 1970s and is used by various computer codes to predict and simulate the transmission and emission of light in gaseous media (with an emphasis on terrestrial and planetary atmospheres). The HITRAN compilation is composed of five major components: the line-by-line spectroscopic parameters required for high-resolution radiative-transfer codes, experimental infrared absorption cross-sections (for molecules where it is not yet feasible for representation in a line-by-line form), collision-induced absorption data, aerosol indices of refraction, and general tables (including partition sums) that apply globally to the data. This paper describes the contents of the 2020 quadrennial edition of HITRAN. The HITRAN2020 edition takes advantage of recent experimental and theoretical data that were meticulously validated, in particular, against laboratory and atmospheric spectra. The new edition replaces the previous HITRAN edition of 2016 (including its updates during the intervening years).
All five components of HITRAN have undergone major updates. In particular, the extent of the updates in the HITRAN2020 edition range from updating a few lines of specific molecules to complete replacements of the lists, and also the introduction of additional isotopologues and new (to HITRAN) molecules: SO, CH3F, GeH4, CS2, CH3I and NF3. Many new vibrational bands were added, extending the spectral coverage and completeness of the line lists. Also, the accuracy of the parameters for major atmospheric absorbers has been increased substantially, often featuring sub-percent uncertainties. Broadening parameters associated with the ambient pressure of water vapor were introduced to HITRAN for the first time and are now available for several molecules.
The HITRAN2020 edition continues to take advantage of the relational structure and efficient interface available at www.hitran.org and the HITRAN Application Programming Interface (HAPI). The functionality of both tools has been extended for the new edition
The prospect of creating medicines based on selenium nanoparticles (Review)
Introduction. The prospects of using nanoparticles in the production of medicines are widely discussed in the literature. In 2018 alone, the quantity of registration certificates issued by national regulators for medicines that use nanoparticles in one form or another is around forty. Most of them are medicines based on liposomes, polymers, iron oxides, micelles. So far, no registration certificates have been issued for selenium nanoparticles. One of the reasons for this situation in this area, from our point of view, is that the mechanisms of interaction of nanoparticles with cells are not sufficiently studied. The lack of basic research in this area is one of the main obstacles to the development of new-generation drugs based on nanoparticles. Text. This review is devoted to the analysis of scientific data on the interaction of selenium nanoparticles with different types of cells. The article discusses the biological properties of selenium and its role in cell metabolism. Data on the cytotoxic effect of selenium nanoparticles on various cell cultures are presented. Methods of preparation of nanoparticles and methods for studying the interaction of nanoparticles with cell cultures are described. Conclusion. Analysis of the literature data allows us to draw conclusions about the relevance of research on the interaction of selenium nanoparticles with living cells. This is necessary to determine the mechanisms of selenium nanoparticles absorption, study their cytotoxic and / or cytostatic action, and distribution in cells. Investigation of the biological interaction of selenium nanoparticles with tumor and normal cells will determine the most informative methods for registering and quantifying their antitumor activity, which is relevant for the development of new drugs to treat cancer. Β© Skorinova K. D., Kuzmenko V. V., Vasilenko I. A., 2020
ΠΠΊΠ½Π΅: ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΏΠΎΠ΄Ρ ΠΎΠ΄Ρ ΠΊ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΡΡΠ°ΡΠΎΠΉ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ
Background. Acne is one of the most common chronic dermatoses, which occurs in almost 8590% of the population. The disease manifests itself as polymorphic eruptions and is characterized by a recurrent and torpid course. Acne treatment is constantly improving, requires an integrated approach and long courses of therapy.
Aims. To increase the effectiveness of acne therapy using new opportunities and promising methodological approaches using a combination of the therapeutic effects of platelet-rich plasma (PRP) and topical bacteriophage therapy.
Materials and methods. The study included 30 patients with papulopustular acne of mild and moderate severity. Patients with mild acne were treated with PRP procedures as monotherapy. For moderate acne, PRP therapy was combined with regular applications of a staphylococcal bacteriophage (NPO Microgen, Nizhny Novgorod, Russia). The treatment was carried out on an outpatient basis: at least 3 procedures with an interval of 12 weeks (up to a maximum of 6 procedures within one year). The results were analyzed using descriptive statistics and the parametric Student's t-test. Data processing was carried out using the Biostat software. The value of the level of statistical significance of differences was taken as p 0.05.
Results. Evaluation of the effectiveness of treatment was carried out on the basis of an analysis of the immediate outcomes of the disease, the dynamics of clinical symptoms and the severity of acne: regression of inflammatory elements of acne, a decrease in the phenomenon of seborrhea, improvement of the skin relief. As a result of the therapy, a transition to remission was noted in 16.6% of patients, an improvement in their condition 36.7%, a significant improvement 46.7%.
Conclusions. An integrated approach combining PRP procedures and bacteriophage therapy can serve as a pathogenetically substantiated strategy for treating acne. A promising direction for further research is the development of new regimens and combinations of alternative therapeutic strategies using bacteriophages.ΠΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅. ΠΠΊΠ½Π΅ ΠΎΠ΄ΠΈΠ½ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΡ
Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
Π΄Π΅ΡΠΌΠ°ΡΠΎΠ·ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠΉ Π²ΡΡΡΠ΅ΡΠ°Π΅ΡΡΡ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Ρ 8590% Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ. ΠΠ°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΠΌΠΈ Π²ΡΡΡΠΏΠ°Π½ΠΈΡΠΌΠΈ ΠΈ ΠΎΡΠ»ΠΈΡΠ°Π΅ΡΡΡ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΈΡΡΡΡΠΈΠΌ ΠΈ ΡΠΎΡΠΏΠΈΠ΄Π½ΡΠΌ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ΠΌ. ΠΠ΅ΡΠ΅Π½ΠΈΠ΅ Π°ΠΊΠ½Π΅ ΠΏΠΎΡΡΠΎΡΠ½Π½ΠΎ ΡΠΎΠ²Π΅ΡΡΠ΅Π½ΡΡΠ²ΡΠ΅ΡΡΡ, ΡΡΠ΅Π±ΡΠ΅Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Π° ΠΈ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΊΡΡΡΠΎΠ² ΡΠ΅ΡΠ°ΠΏΠΈΠΈ.
Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠΎΠ²ΡΡΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π°ΠΊΠ½Π΅, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡ Π½ΠΎΠ²ΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ Π² ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ Π»Π΅ΡΠ΅Π±Π½ΡΠΌ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ ΠΏΠ»Π°Π·ΠΌΡ, ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΠΎΠΉ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ, ΠΈ ΡΠΎΠΏΠΈΡΠ΅ΡΠΊΠΎΠΉ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΡΠ΅ΡΠ°ΠΏΠΈΠ΅ΠΉ.
ΠΠ΅ΡΠΎΠ΄Ρ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π±ΡΠ»ΠΈ Π²ΠΊΠ»ΡΡΠ΅Π½Ρ 30 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΠ°ΠΏΡΠ»ΠΎ-ΠΏΡΡΡΡΠ»Π΅Π·Π½ΠΎΠΉ ΡΠΎΡΠΌΠΎΠΉ Π°ΠΊΠ½Π΅ Π»Π΅Π³ΠΊΠΎΠΉ ΠΈ ΡΡΠ΅Π΄Π½Π΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΡΠΆΠ΅ΡΡΠΈ. ΠΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌ Ρ Π°ΠΊΠ½Π΅ Π»Π΅Π³ΠΊΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΡΠΆΠ΅ΡΡΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΌΠΎΠ½ΠΎΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π½Π°Π·Π½Π°ΡΠ°Π»ΠΈ PRP-ΠΏΡΠΎΡΠ΅Π΄ΡΡΡ. ΠΡΠΈ Π°ΠΊΠ½Π΅ ΡΡΠ΅Π΄Π½Π΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΡΠΆΠ΅ΡΡΠΈ PRP-ΡΠ΅ΡΠ°ΠΏΠΈΡ ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π»ΠΈ Ρ ΡΠ΅Π³ΡΠ»ΡΡΠ½ΡΠΌΠΈ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΡΠΌΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³Π° ΡΡΠ°ΡΠΈΠ»ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠ³ΠΎ (ΠΠΠ ΠΠΈΠΊΡΠΎΠ³Π΅Π½, ΠΠΈΠΆΠ½ΠΈΠΉ ΠΠΎΠ²Π³ΠΎΡΠΎΠ΄, Π ΠΎΡΡΠΈΡ). ΠΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π°ΠΌΠ±ΡΠ»Π°ΡΠΎΡΠ½ΠΎ: Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ 3 ΠΏΡΠΎΡΠ΅Π΄ΡΡ Ρ ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»ΠΎΠΌ 12 Π½Π΅Π΄Π΅Π»ΠΈ (ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎ Π΄ΠΎ 6 ΠΏΡΠΎΡΠ΅Π΄ΡΡ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π³ΠΎΠ΄Π°). ΠΠ½Π°Π»ΠΈΠ· ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΎΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΡΠ°ΡΠΈΡΡΠΈΠΊΠΈ ΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ t-ΠΊΡΠΈΡΠ΅ΡΠΈΡ Π‘ΡΡΡΠ΄Π΅Π½ΡΠ°. ΠΠ±ΡΠ°Π±ΠΎΡΠΊΡ Π΄Π°Π½Π½ΡΡ
ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ Biostat. ΠΠ° Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΡΡΠΎΠ²Π½Ρ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΠΈ ΡΠ°Π·Π»ΠΈΡΠΈΠΉ ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π»ΠΈ p 0,05.
Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠ΅Π½ΠΊΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ Π°Π½Π°Π»ΠΈΠ·Π° Π±Π»ΠΈΠΆΠ°ΠΉΡΠΈΡ
ΠΈΡΡ
ΠΎΠ΄ΠΎΠ² Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ, Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΈΠΌΠΏΡΠΎΠΌΠΎΠ² ΠΈ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΡΠΆΠ΅ΡΡΠΈ Π°ΠΊΠ½Π΅: ΡΠ΅Π³ΡΠ΅ΡΡΠ° Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΡΠ³ΡΠ΅Π²ΠΎΠΉ ΡΡΠΏΠΈ, ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΡΠ²Π»Π΅Π½ΠΈΡ ΡΠ΅Π±ΠΎΡΠ΅ΠΈ, ΡΠ»ΡΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΠΆΠ½ΠΎΠ³ΠΎ ΡΠ΅Π»ΡΠ΅ΡΠ°. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠΌΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄ Π² ΡΠ΅ΠΌΠΈΡΡΠΈΡ ΠΎΡΠΌΠ΅ΡΠ΅Π½ Ρ 16,6% ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², ΡΠ»ΡΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ Ρ 36,7%, Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠ»ΡΡΡΠ΅Π½ΠΈΠ΅ Ρ 46,7%.
ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈ ΠΎΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΡΡΠ°ΡΠ΅Π³ΠΈΠ΅ΠΉ Π»Π΅ΡΠ΅Π½ΠΈΡ Π°ΠΊΠ½Π΅ ΠΌΠΎΠΆΠ΅Ρ ΡΠ»ΡΠΆΠΈΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΡΠΉ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄, ΡΠΎΡΠ΅ΡΠ°ΡΡΠΈΠΉ PRP-ΠΏΡΠΎΡΠ΅Π΄ΡΡΡ ΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΡΠ΅ΡΠ°ΠΏΠΈΡ. ΠΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° Π½ΠΎΠ²ΡΡ
ΡΠ΅ΠΆΠΈΠΌΠΎΠ² ΠΈ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΉ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΡΡ
ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΡΠ°ΡΠ΅Π³ΠΈΠΉ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ²
Prevention of thrombohemorrhagic postoperative complications in patients with benign prostatic hyperplasia
AIM: To improve the results of surgical treatment of benign prostatic hyperplasia.MATERIALS AND METHODS: The study investigated the effectiveness of a comprehensive preoperative preparation of patients with benign prostatic hyperplasia. The clinical efficacy of traditional methods of preoperative preparation (compression bandaging of the lower extremities during surgery and in the postoperative period and Fraxiparine at a prophylactic dose) was compared with the same preoperative protocol used in combination with intravenous laser blood irradiation. The explored parameters included changes in clinical and laboratory coagulation indices and prostatic blood flow measured by Doppler sonography. The real time visualization was used to assess the effect of intravenous laser irradiation of blood on the morphofunctional state of platelets.CONCLUSION: The study findings showed a high effectiveness of intravenous laser blood irradiation in preoperative preparation of patients with benign prostatic hyperplasia. It was found to reduce the incidence of thrombotic events by 6% and hemorrhagic complications by 4.9% (p<0.05)
Prevention of thrombohemorrhagic postoperative complications in patients with benign prostatic hyperplasia
AIM: To improve the results of surgical treatment of benign prostatic hyperplasia.MATERIALS AND METHODS: The study investigated the effectiveness of a comprehensive preoperative preparation of patients with benign prostatic hyperplasia. The clinical efficacy of traditional methods of preoperative preparation (compression bandaging of the lower extremities during surgery and in the postoperative period and Fraxiparine at a prophylactic dose) was compared with the same preoperative protocol used in combination with intravenous laser blood irradiation. The explored parameters included changes in clinical and laboratory coagulation indices and prostatic blood flow measured by Doppler sonography. The real time visualization was used to assess the effect of intravenous laser irradiation of blood on the morphofunctional state of platelets.CONCLUSION: The study findings showed a high effectiveness of intravenous laser blood irradiation in preoperative preparation of patients with benign prostatic hyperplasia. It was found to reduce the incidence of thrombotic events by 6% and hemorrhagic complications by 4.9% (p<0.05)
ΠΡΠ΅Π½ΠΊΠ° ΡΠ΅Π½ΠΎΡΠΈΠΏΠ° ΠΈΠ½ΡΠ΅ΡΡΠ°Π·Π½ΡΡ ΡΠ΄Π΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠ°Π·ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠΌΠΈΠ΄ΠΆΠΈΠ½Π³Π° (QPI) Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ Ρ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΎΠΈΠ΄Π½ΡΠΌΠΈ ΠΊΠΈΡΡΠ°ΠΌΠΈ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ²
Rationale: Ovarian endometriosis is a progressive disease with growing prevalence and severity. Therefore, the development of robust non-invasive laboratory screening methods for early diagnosis on the out-patient basis seems quite relevant. Aim: To assess a potential of the quantitative phase imaging technique for early diagnosis of ovarian endometrial cysts and post-operative relapses of the disease. Materials and methods: We analyzed 1578 nuclei of the peripheral blood lymphocytes from 82 patients with ovarian endometrial cysts, aged 21 to 37 years (mean age 26.4 Β± 3.6 years). The patients were follow-up in a gynecology out-patient clinic (the town of Yessentuki, Russia). Assessments were made longitudinally, i.e., before a laparoscopic cystectomy, at 6 and 12 months in the post-operative period with or without treatment with dienogest-containing agents. Morphological and functional status of the nuclei from the peripheral blood lymphocytes was assessed in the real-time mode by quantitative phase imaging (QPI) with the phase-interference microscopy module of the Bioni hardware and software complex (Westgrade Ltd., Moscow) for clinical and laboratory diagnostics, and the morphodensitometric segmentation technology. Results: The comparative analysis of morphometric parameters of CD3sup+/sup cells taken from peripheral blood of healthy non-pregnant women and patients with ovarian endometrial cysts before surgery showed a significant increase of the calculated functional activities of the lymphocyte nuclei (0.898 vs 0.783, p 0.05). Assessment of changes overt time in the differential diagnostic criteria of the nuclear response in the peripheral blood lymphocytes from patients with endometrial ovarian cysts showed the following. Compared to the parameters obtained before treatment, at 6 and 12 months of the post-operative period the relative intensity of nuclear segments (ΞI) decreased by 10.3 and 14.7, 10.6 and 12.9% in the group treated with and without dienogest, respectively. Relative distance between the centers of the nuclear segments (ΞL) demonstrated a trend towards an increase by 0.6 and 0.9, 4.2 and 2.1%. The numbers of nuclear segments increased by 18.3 and 13.4, 27.4 and 16.9%, whereas the nuclear perimeter decreased by 13.9 and 12.6, 11.9 and 7.8%, respectively. In the patients treated with dienogest, the rate of non-relapse at 6 and 12 months of the follow-up was 100%, whereas in the patients without dienogest therapy, 97.5 and 93.5%, respectively. Discussion: Interphase chromatin is a unique biosensor of the early abnormalities in a lymphoid cell. Modification of its structure and packaging density not only indicate changes of the morphofunctional status of the lymphocyte, but can be projected to the body as a whole and used for early pre-clinical diagnosis, assessment of severity of the pathological process and prediction of the outcome in various critic states. Conclusion: Practical implementation of QPI for clinical monitoring of patients with ovarian endometrial cysts makes it possible to obtain important information on the cell immunity in real time. It opens new opportunities to assess the efficacy of treatment and rehabilitation activities, as well as for early pre-clinical diagnosis of relapsing disease.Β ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΎΠ· ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² β ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΡΡΡΡΠ΅Π΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠ΅, ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΡ ΠΈ ΡΡΠΆΠ΅ΡΡΡ ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ Π½Π΅ΡΠΊΠ»ΠΎΠ½Π½ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ°ΡΡ. Π ΡΠ²ΡΠ·ΠΈ Ρ ΡΡΠΈΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠΌΠΈ Π²ΠΎΠΏΡΠΎΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π½Π°Π΄Π΅ΠΆΠ½ΡΡ
Π½Π΅ΠΈΠ½Π²Π°Π·ΠΈΠ²Π½ΡΡ
ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ΠΎΠ²ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Π½Π° ΡΡΠ°ΠΏΠ΅ ΡΠ°Π½Π½Π΅Π³ΠΎ Π°ΠΌΠ±ΡΠ»Π°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. Π¦Π΅Π»Ρ β ΠΎΡΠ΅Π½ΠΊΠ° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠ΅ΠΉ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠ°Π·ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠΌΠΈΠ΄ΠΆΠΈΠ½Π³Π° Π΄Π»Ρ ΡΠ°Π½Π½Π΅ΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΎΠΈΠ΄Π½ΡΡ
ΠΊΠΈΡΡ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² ΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΎΠ² Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Π² ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Ρ 1578 ΡΠ΄Π΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ 82 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ Ρ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΎΠΈΠ΄Π½ΡΠΌΠΈ ΠΊΠΈΡΡΠ°ΠΌΠΈ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 21 Π΄ΠΎ 37 Π»Π΅Ρ (ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ 26,4 Β± 3,6 Π³ΠΎΠ΄Π°), Π½Π°Π±Π»ΡΠ΄Π°Π²ΡΠΈΡ
ΡΡ Π² ΠΆΠ΅Π½ΡΠΊΠΎΠΉ ΠΊΠΎΠ½ΡΡΠ»ΡΡΠ°ΡΠΈΠΈ (Π³. ΠΡΡΠ΅Π½ΡΡΠΊΠΈ). ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π² Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅: Π΄ΠΎ Π»Π°ΠΏΠ°ΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠΈΡΡΡΠΊΡΠΎΠΌΠΈΠΈ, ΡΠ΅ΡΠ΅Π· 6 ΠΈ 12 ΠΌΠ΅ΡΡΡΠ΅Π² ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΠΈΠΎΠ΄Π° Π½Π° ΡΠΎΠ½Π΅ ΠΈΠ»ΠΈ Π±Π΅Π· Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°ΠΌΠΈ Ρ Π΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΠΌ Π²Π΅ΡΠ΅ΡΡΠ²ΠΎΠΌ Π΄ΠΈΠ΅Π½ΠΎΠ³Π΅ΡΡ. ΠΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ ΡΠ΄Π΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠ°Π·ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠΌΠΈΠ΄ΠΆΠΈΠ½Π³Π° (QPI) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠΎΠ΄ΡΠ»Ρ ΡΠ°Π·ΠΎΠ²ΠΎ-ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅Π½ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ Π°ΠΏΠΏΠ°ΡΠ°ΡΠ½ΠΎ-ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° Β«ΠΠΈΠΎΠ½ΠΈΒ» (ΠΠΠ Β«ΠΠ΅ΡΡΡΡΠ΅ΠΉΠ΄Β», ΠΠΎΡΠΊΠ²Π°) Π΄Π»Ρ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΌΠΎΡΡΠΎΠ΄Π΅Π½ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π³ΠΌΠ΅Π½ΡΠ°ΡΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π΅ ΠΌΠΎΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ CD3+-ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π½Π΅Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΠΆΠ΅Π½ΡΠΈΠ½ ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ Ρ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΎΠΈΠ΄Π½ΡΠΌΠΈ ΠΊΠΈΡΡΠ°ΠΌΠΈ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² Π΄ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΈΠΌ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ Π²ΡΡΠ²Π»Π΅Π½ΠΎ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ°ΡΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΄Π΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² (0,898 ΠΏΡΠΎΡΠΈΠ² 0,783, p 0,05). ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ-Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π² ΡΠ΅Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΡΠ΄Π΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ Ρ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΎΠΈΠ΄Π½ΡΠΌΠΈ ΠΊΠΈΡΡΠ°ΠΌΠΈ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ: ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°ΠΌΠΈ Π΄ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ Π½Π° 6-ΠΌ ΠΈ 12-ΠΌ ΠΌΠ΅ΡΡΡΠ°Ρ
ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΠΈΠΎΠ΄Π° Π²Π΅Π»ΠΈΡΠΈΠ½Π° ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅Π³ΠΌΠ΅Π½ΡΠΎΠ² Π² ΡΠ΄ΡΠ°Ρ
(ΞI) ΡΠ½ΠΈΠΆΠ°Π»Π°ΡΡ Π½Π° 10,3 ΠΈ 14,7, 10,6 ΠΈ 12,9% Π² Π³ΡΡΠΏΠΏΠ°Ρ
, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
ΡΠ΅ΡΠ°ΠΏΠΈΡ Π΄ΠΈΠ΅Π½ΠΎΠ³Π΅ΡΡΠΎΠΌ, ΠΈ Π±Π΅Π· ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π΄ΠΈΠ΅Π½ΠΎΠ³Π΅ΡΡΠΎΠΌ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ; ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠ°ΡΡΡΠΎΡΠ½ΠΈΠ΅ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠ΅Π½ΡΡΠ°ΠΌΠΈ ΡΠ΅Π³ΠΌΠ΅Π½ΡΠΎΠ² ΡΠ΄Π΅Ρ (ΞL) ΠΈΠΌΠ΅Π»ΠΎ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ ΠΊ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ Π½Π° 0,6 ΠΈ 0,9, 4,2 ΠΈ 2,1%; ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΠ΅Π³ΠΌΠ΅Π½ΡΠΎΠ² Π² ΡΠ΄ΡΠ°Ρ
ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°Π»ΠΎΡΡ Π½Π° 18,3 ΠΈ 13,4, 27,4 ΠΈ 16,9%; ΠΏΠ΅ΡΠΈΠΌΠ΅ΡΡ ΡΠ΄Π΅Ρ ΡΠΌΠ΅Π½ΡΡΠ°Π»ΡΡ Π½Π° 13,9 ΠΈ 12,6, 11,9 ΠΈ 7,8% ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. Π§Π°ΡΡΠΎΡΠ° Π±Π΅Π·ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΡΡ
ΡΠ»ΡΡΠ°Π΅Π² ΡΠ΅ΡΠ΅Π· 6 ΠΈ 12 ΠΌΠ΅ΡΡΡΠ΅Π² Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ Π² Π³ΡΡΠΏΠΏΠ°Ρ
Π±ΠΎΠ»ΡΠ½ΡΡ
, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
Π΄ΠΈΠ΅Π½ΠΎΠ³Π΅ΡΡ, ΡΠΎΡΡΠ°Π²ΠΈΠ»Π° 100%, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ, Π½Π΅ ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π΄ΠΈΠ΅Π½ΠΎΠ³Π΅ΡΡΠΎΠΌ, β 97,5 ΠΈ 93,5% ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. ΠΠ½ΡΠ΅ΡΡΠ°Π·Π½ΡΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΈΠ½ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΡΠΎΠ±ΠΎΠΉ ΡΠ²ΠΎΠ΅ΠΎΠ±ΡΠ°Π·Π½ΡΠΉ Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡ, Π΄Π°ΡΡΠΈΠΊ ΡΠ°Π½Π½ΠΈΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π»ΠΈΠΌΡΠΎΠΈΠ΄Π½ΠΎΠΉ ΠΊΠ»Π΅ΡΠΊΠΈ. ΠΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π΅Π³ΠΎ ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΡΠΏΠ°ΠΊΠΎΠ²ΠΊΠΈ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎΠ± ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°, Π½ΠΎ ΠΈ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΡΠΏΡΠΎΠ΅ΡΠΈΡΠΎΠ²Π°Π½Ρ Π½Π° ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌ Π² ΡΠ΅Π»ΠΎΠΌ Π΄Π»Ρ ΡΠ°Π½Π½Π΅ΠΉ Π΄ΠΎΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ, ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΠΆΠ΅ΡΡΠΈ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΠ·Π° ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΊΡΠΈΠ·ΠΈΡΠ½ΡΡ
ΡΠΎΡΡΠΎΡΠ½ΠΈΡΡ
. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ QPI Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΌΠΎΠ½ΠΈΡΠΎΡΠΈΠ½Π³Π΅ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΎΠΈΠ΄Π½ΡΠΌΠΈ ΠΊΠΈΡΡΠ°ΠΌΠΈ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΌΡ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π²Π°ΠΆΠ½ΠΎΠΉ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ Π·Π²Π΅Π½Π° ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ°, ΠΎΡΠΊΡΡΠ²Π°Π΅Ρ Π½ΠΎΠ²ΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠΌΡΡ
Π»Π΅ΡΠ΅Π±Π½ΡΡ
ΠΈ ΡΠ΅Π°Π±ΠΈΠ»ΠΈΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ
ΠΌΠ΅ΡΠΎΠΏΡΠΈΡΡΠΈΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ°Π½Π½Π΅ΠΉ Π΄ΠΎΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π° Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ