THE ISSUE OF PREGNANCY AND DELIVERY WITH PRENATAL INFECTION OF THE FETUS, HEALTH STATUS OF BABIES ON THE FIRST YEARS OF LIFE

Pregnancy and delivery of 27 women with prenatal infection of the fetus, neonatal period of the babies with prenatal pneumonia, health status of up to 2 children were researched in retrospect. Women with inflammation of the genitals (78,6%) and extragenital pathology of the inflammatory genesis (36,3%) had prenatal infection of the fetus. In the early neonatal period every baby had cerebral ischemia, every third child had haemorrhages in different cerebral structures. All babies have been transferred to children's hospital for treatment where the rehabilitation was carried out during 1-2,5 months. Infantile cerebral paralysis, hydrocephaly, epilepsy, epileptoid attacks were detected in 8 babies on the first year of life (29,6%). 5 babies (18,5%) had atrophy of discs of optic nerves, retinal angiopathy. All babies with local pathology of the central nervous system were premature born on the 26-32 week. Majority has herniae of different localization (55,5%), congenital dysplasia of the hip joint (74%), 26 babies (96,2%) were on the books because of anaemiae, pneumoniae, bronchites, pyelonephrites, septicaemiae. Using the received data we can draw a conclusion: in order to reduce perinatal complications in the maternal organism, in the organism of newborn baby and baby on the first year of life it is necessary to sanify the birth canal, to do preclinical diagnostics and preventive therapy of the prematurely born, gestosis, fetoplacental insufficiency

The HITRAN2020 Molecular Spectroscopic Database

The HITRAN database is a compilation of molecular spectroscopic parameters. It was established in the early 1970s and is used by various computer codes to predict and simulate the transmission and emission of light in gaseous media (with an emphasis on terrestrial and planetary atmospheres). The HITRAN compilation is composed of five major components: the line-by-line spectroscopic parameters required for high-resolution radiative-transfer codes, experimental infrared absorption cross-sections (for molecules where it is not yet feasible for representation in a line-by-line form), collision-induced absorption data, aerosol indices of refraction, and general tables (including partition sums) that apply globally to the data. This paper describes the contents of the 2020 quadrennial edition of HITRAN. The HITRAN2020 edition takes advantage of recent experimental and theoretical data that were meticulously validated, in particular, against laboratory and atmospheric spectra. The new edition replaces the previous HITRAN edition of 2016 (including its updates during the intervening years). All five components of HITRAN have undergone major updates. In particular, the extent of the updates in the HITRAN2020 edition range from updating a few lines of specific molecules to complete replacements of the lists, and also the introduction of additional isotopologues and new (to HITRAN) molecules: SO, CH3F, GeH4, CS2, CH3I and NF3. Many new vibrational bands were added, extending the spectral coverage and completeness of the line lists. Also, the accuracy of the parameters for major atmospheric absorbers has been increased substantially, often featuring sub-percent uncertainties. Broadening parameters associated with the ambient pressure of water vapor were introduced to HITRAN for the first time and are now available for several molecules. The HITRAN2020 edition continues to take advantage of the relational structure and efficient interface available at www.hitran.org and the HITRAN Application Programming Interface (HAPI). The functionality of both tools has been extended for the new edition

The prospect of creating medicines based on selenium nanoparticles (Review)

Introduction. The prospects of using nanoparticles in the production of medicines are widely discussed in the literature. In 2018 alone, the quantity of registration certificates issued by national regulators for medicines that use nanoparticles in one form or another is around forty. Most of them are medicines based on liposomes, polymers, iron oxides, micelles. So far, no registration certificates have been issued for selenium nanoparticles. One of the reasons for this situation in this area, from our point of view, is that the mechanisms of interaction of nanoparticles with cells are not sufficiently studied. The lack of basic research in this area is one of the main obstacles to the development of new-generation drugs based on nanoparticles. Text. This review is devoted to the analysis of scientific data on the interaction of selenium nanoparticles with different types of cells. The article discusses the biological properties of selenium and its role in cell metabolism. Data on the cytotoxic effect of selenium nanoparticles on various cell cultures are presented. Methods of preparation of nanoparticles and methods for studying the interaction of nanoparticles with cell cultures are described. Conclusion. Analysis of the literature data allows us to draw conclusions about the relevance of research on the interaction of selenium nanoparticles with living cells. This is necessary to determine the mechanisms of selenium nanoparticles absorption, study their cytotoxic and / or cytostatic action, and distribution in cells. Investigation of the biological interaction of selenium nanoparticles with tumor and normal cells will determine the most informative methods for registering and quantifying their antitumor activity, which is relevant for the development of new drugs to treat cancer. © Skorinova K. D., Kuzmenko V. V., Vasilenko I. A., 2020

Акне: современные подходы к решению старой проблемы

Background. Acne is one of the most common chronic dermatoses, which occurs in almost 8590% of the population. The disease manifests itself as polymorphic eruptions and is characterized by a recurrent and torpid course. Acne treatment is constantly improving, requires an integrated approach and long courses of therapy. Aims. To increase the effectiveness of acne therapy using new opportunities and promising methodological approaches using a combination of the therapeutic effects of platelet-rich plasma (PRP) and topical bacteriophage therapy. Materials and methods. The study included 30 patients with papulopustular acne of mild and moderate severity. Patients with mild acne were treated with PRP procedures as monotherapy. For moderate acne, PRP therapy was combined with regular applications of a staphylococcal bacteriophage (NPO Microgen, Nizhny Novgorod, Russia). The treatment was carried out on an outpatient basis: at least 3 procedures with an interval of 12 weeks (up to a maximum of 6 procedures within one year). The results were analyzed using descriptive statistics and the parametric Student's t-test. Data processing was carried out using the Biostat software. The value of the level of statistical significance of differences was taken as p 0.05. Results. Evaluation of the effectiveness of treatment was carried out on the basis of an analysis of the immediate outcomes of the disease, the dynamics of clinical symptoms and the severity of acne: regression of inflammatory elements of acne, a decrease in the phenomenon of seborrhea, improvement of the skin relief. As a result of the therapy, a transition to remission was noted in 16.6% of patients, an improvement in their condition 36.7%, a significant improvement 46.7%. Conclusions. An integrated approach combining PRP procedures and bacteriophage therapy can serve as a pathogenetically substantiated strategy for treating acne. A promising direction for further research is the development of new regimens and combinations of alternative therapeutic strategies using bacteriophages.Обоснование. Акне один из наиболее распространенных хронических дерматозов, который встречается практически у 8590% населения. Заболевание проявляется полиморфными высыпаниями и отличается рецидивирующим и торпидным течением. Лечение акне постоянно совершенствуется, требует комплексного подхода и длительных курсов терапии. Цель исследования. Повысить эффективность терапии акне, используя новые возможности и перспективные методические подходы в комбинации с лечебным воздействием плазмы, обогащенной тромбоцитами, и топической бактериофаготерапией. Методы. В исследование были включены 30 пациентов с папуло-пустулезной формой акне легкой и средней степени тяжести. Пациентам с акне легкой степени тяжести в качестве монотерапии назначали PRP-процедуры. При акне средней степени тяжести PRP-терапию комбинировали с регулярными аппликациями препаратом бактериофага стафилококкового (НПО Микроген, Нижний Новгород, Россия). Лечение проводили амбулаторно: не менее 3 процедур с интервалом 12 недели (максимально до 6 процедур в течение одного года). Анализ полученных результатов проводили с использованием описательной статистики и применением параметрического t-критерия Стьюдента. Обработку данных осуществляли с помощью программы Biostat. За величину уровня статистической значимости различий принимали p 0,05. Результаты. Оценку эффективности лечения проводили на основании анализа ближайших исходов заболевания, динамики клинических симптомов и степени тяжести акне: регресса воспалительных элементов угревой сыпи, снижения явления себореи, улучшения кожного рельефа. В результате проводимой терапии переход в ремиссию отмечен у 16,6% пациентов, улучшение состояния у 36,7%, значительное улучшение у 46,7%. Заключение. Патогенетически обоснованной стратегией лечения акне может служить комплексный подход, сочетающий PRP-процедуры и бактериофаготерапию. Перспективным направлением дальнейших исследований является разработка новых режимов и комбинаций альтернативных терапевтических стратегий с применением бактериофагов

Prevention of thrombohemorrhagic postoperative complications in patients with benign prostatic hyperplasia

AIM: To improve the results of surgical treatment of benign prostatic hyperplasia.MATERIALS AND METHODS: The study investigated the effectiveness of a comprehensive preoperative preparation of patients with benign prostatic hyperplasia. The clinical efficacy of traditional methods of preoperative preparation (compression bandaging of the lower extremities during surgery and in the postoperative period and Fraxiparine at a prophylactic dose) was compared with the same preoperative protocol used in combination with intravenous laser blood irradiation. The explored parameters included changes in clinical and laboratory coagulation indices and prostatic blood flow measured by Doppler sonography. The real time visualization was used to assess the effect of intravenous laser irradiation of blood on the morphofunctional state of platelets.CONCLUSION: The study findings showed a high effectiveness of intravenous laser blood irradiation in preoperative preparation of patients with benign prostatic hyperplasia. It was found to reduce the incidence of thrombotic events by 6% and hemorrhagic complications by 4.9% (p<0.05)

Prevention of thrombohemorrhagic postoperative complications in patients with benign prostatic hyperplasia

AIM: To improve the results of surgical treatment of benign prostatic hyperplasia.MATERIALS AND METHODS: The study investigated the effectiveness of a comprehensive preoperative preparation of patients with benign prostatic hyperplasia. The clinical efficacy of traditional methods of preoperative preparation (compression bandaging of the lower extremities during surgery and in the postoperative period and Fraxiparine at a prophylactic dose) was compared with the same preoperative protocol used in combination with intravenous laser blood irradiation. The explored parameters included changes in clinical and laboratory coagulation indices and prostatic blood flow measured by Doppler sonography. The real time visualization was used to assess the effect of intravenous laser irradiation of blood on the morphofunctional state of platelets.CONCLUSION: The study findings showed a high effectiveness of intravenous laser blood irradiation in preoperative preparation of patients with benign prostatic hyperplasia. It was found to reduce the incidence of thrombotic events by 6% and hemorrhagic complications by 4.9% (p<0.05)

Оценка фенотипа интерфазных ядер лимфоцитов методом количественного фазового имиджинга (QPI) у пациенток с эндометриоидными кистами яичников

Rationale: Ovarian endometriosis is a progressive disease with growing prevalence and severity. Therefore, the development of robust non-invasive laboratory screening methods for early diagnosis on the out-patient basis seems quite relevant. Aim: To assess a potential of the quantitative phase imaging technique for early diagnosis of ovarian endometrial cysts and post-operative relapses of the disease. Materials and methods: We analyzed 1578 nuclei of the peripheral blood lymphocytes from 82 patients with ovarian endometrial cysts, aged 21 to 37 years (mean age 26.4 ± 3.6 years). The patients were follow-up in a gynecology out-patient clinic (the town of Yessentuki, Russia). Assessments were made longitudinally, i.e., before a laparoscopic cystectomy, at 6 and 12 months in the post-operative period with or without treatment with dienogest-containing agents. Morphological and functional status of the nuclei from the peripheral blood lymphocytes was assessed in the real-time mode by quantitative phase imaging (QPI) with the phase-interference microscopy module of the Bioni hardware and software complex (Westgrade Ltd., Moscow) for clinical and laboratory diagnostics, and the morphodensitometric segmentation technology. Results: The comparative analysis of morphometric parameters of CD3sup+/sup cells taken from peripheral blood of healthy non-pregnant women and patients with ovarian endometrial cysts before surgery showed a significant increase of the calculated functional activities of the lymphocyte nuclei (0.898 vs 0.783, p 0.05). Assessment of changes overt time in the differential diagnostic criteria of the nuclear response in the peripheral blood lymphocytes from patients with endometrial ovarian cysts showed the following. Compared to the parameters obtained before treatment, at 6 and 12 months of the post-operative period the relative intensity of nuclear segments (ΔI) decreased by 10.3 and 14.7, 10.6 and 12.9% in the group treated with and without dienogest, respectively. Relative distance between the centers of the nuclear segments (ΔL) demonstrated a trend towards an increase by 0.6 and 0.9, 4.2 and 2.1%. The numbers of nuclear segments increased by 18.3 and 13.4, 27.4 and 16.9%, whereas the nuclear perimeter decreased by 13.9 and 12.6, 11.9 and 7.8%, respectively. In the patients treated with dienogest, the rate of non-relapse at 6 and 12 months of the follow-up was 100%, whereas in the patients without dienogest therapy, 97.5 and 93.5%, respectively. Discussion: Interphase chromatin is a unique biosensor of the early abnormalities in a lymphoid cell. Modification of its structure and packaging density not only indicate changes of the morphofunctional status of the lymphocyte, but can be projected to the body as a whole and used for early pre-clinical diagnosis, assessment of severity of the pathological process and prediction of the outcome in various critic states. Conclusion: Practical implementation of QPI for clinical monitoring of patients with ovarian endometrial cysts makes it possible to obtain important information on the cell immunity in real time. It opens new opportunities to assess the efficacy of treatment and rehabilitation activities, as well as for early pre-clinical diagnosis of relapsing disease. Актуальность. Эндометриоз яичников – прогрессирующее заболевание, распространенность и тяжесть которого неуклонно возрастают. В связи с этим представляются актуальными вопросы разработки надежных неинвазивных скрининговых методов лабораторной диагностики заболевания на этапе раннего амбулаторного обследования. Цель – оценка возможностей метода количественного фазового имиджинга для ранней диагностики эндометриоидных кист яичников и рецидивов заболевания в послеоперационном периоде. Материал и методы. Проанализированы 1578 ядер лимфоцитов периферической крови 82 пациенток с эндометриоидными кистами яичников в возрасте от 21 до 37 лет (средний возраст 26,4 ± 3,6 года), наблюдавшихся в женской консультации (г. Ессентуки). Исследования проводили в динамике: до лапароскопической цистэктомии, через 6 и 12 месяцев послеоперационного периода на фоне или без лечения препаратами с действующим веществом диеногест. Морфофункциональное состояние ядер лимфоцитов периферической крови оценивали в режиме реального времени методом количественного фазового имиджинга (QPI) с использованием модуля фазово-интерференционной микроскопии аппаратно-программного комплекса «Биони» (ООО «Весттрейд», Москва) для клинической и лабораторной диагностики, а также технологии морфоденситометрической сегментации. Результаты. При сравнительном анализе морфометрических показателей CD3+-клеток периферической крови соматически здоровых небеременных женщин и пациенток с эндометриоидными кистами яичников до проведения им оперативного лечения выявлено статистически значимое повышение расчетного показателя функциональной активности ядер лимфоцитов (0,898 против 0,783, p 0,05). Исследование динамики дифференциально-диагностических критериев реактивных изменений ядер лимфоцитов периферической крови пациенток с эндометриоидными кистами яичников показало: по сравнению с результатами до лечения на 6-м и 12-м месяцах послеоперационного периода величина относительной интенсивности сегментов в ядрах (ΔI) снижалась на 10,3 и 14,7, 10,6 и 12,9% в группах, получавших терапию диеногестом, и без терапии диеногестом соответственно; относительное расстояние между центрами сегментов ядер (ΔL) имело тенденцию к увеличению на 0,6 и 0,9, 4,2 и 2,1%; количество сегментов в ядрах увеличивалось на 18,3 и 13,4, 27,4 и 16,9%; периметр ядер уменьшался на 13,9 и 12,6, 11,9 и 7,8% соответственно. Частота безрецидивных случаев через 6 и 12 месяцев наблюдения в группах больных, получавших диеногест, составила 100%, тогда как у пациенток, не получавших лечение диеногестом, – 97,5 и 93,5% соответственно. Обсуждение. Интерфазный хроматин представляет собой своеобразный биосенсор, датчик ранних изменений лимфоидной клетки. Модификации его структуры и плотности упаковки не только свидетельствуют об изменении морфофункционального состояния лимфоцита, но и могут быть спроецированы на организм в целом для ранней доклинической диагностики, оценки тяжести патологического процесса и прогноза при различных кризисных состояниях. Заключение. Практическое использование QPI в клиническом мониторинге больных с эндометриоидными кистами яичников способствует оперативному получению важной информации о состоянии клеточного звена иммунитета, открывает новые возможности для оценки эффективности проводимых лечебных и реабилитационных мероприятий, а также ранней доклинической диагностики рецидива заболевания