Manejo de animais e pastagens em sistemas de integração silvipastoril

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Los sistemas silvopastoriles en las regiones subtropicales del Brasil: las actividades de la EMBRAPA.

En Ias regiones subtropicales dei Brasil hay una gran oportunidad para el establecimiento de sistemas silvopastoriles. En esta región, Ias sistemas dominantes de uso de Ia tierra son Ias actividades agrícolas (maíz, soja, trigo, frijol, arroz, etc.) y ganaderas (producción de carne y leche bovina y ovina). Hay presiones económicas y ambientales que actúan en el media rural sobre Ias productores tendientes a adaptar sistemas de uso de Ia tierra más sostenibles. Si bien existen pruebas científicas y ejemplos de aplicación, Ia diversidad de condiciones regionales aconsejan desarrollar más estudios y mecanismos de política pública para promover Ia conversión de Ias pastizales sin árboles aios sistemas silvopastoriles

Insulin-like growth factor receptor 1 (IGF1R) expression and survival in surgically resected non-small-cell lung cancer (NSCLC) patients

Background: The purpose of this study is to investigate the prognostic role of insulin-like growth factor receptor 1 (IGF1R) expression in surgically resected non-small-cell lung cancer (NSCLC). Patient characteristics and methods: This retrospective study was conducted in 369 stage I-II-IIIA, surgically resected, NSCLC patients. Patients exposed to anti-epidermal growth factor receptor (EGFR) agents were excluded. IGF1R expression was evaluated by immunohistochemistry in tissue microarray sections. Results: A positive IGF1R expression (score ≥ 100) was observed in 282 cases (76.4%) and was significantly associated with squamous cell histology (P = 0.04) and with grade III differentiation (P = 0.02). No difference in survival was observed between the positive and negative group when score 100 was used as cut-off for discriminating a positive versus a negative IGF1R result (52 versus 48 months, P = 0.99) or when median value of IGF1R expression was used (45 versus 55 months, P = 0.36). No difference in survival was observed between IGF1R-positive and -negative patients in a subgroup of stage I-II adenocarcinoma (n = 137) with known EGFR mutation and copy number status. Conclusions: IGF1R expression does not represent a prognostic factor in resected NSCLC patients. Patients with squamous cell carcinoma overexpress IGF1R more frequently than patients with nonsquamous histology, justifying the different sensitivity to anti-IGF1R agents observed in clinical trial

KIAA1549: BRAF gene fusion and FGFR1 hotspot mutations are prognostic factors in pilocytic astrocytomas

Up to 20% of patients with pilocytic astrocytoma (PA) experience a poor outcome. BRAF alterations and Fibroblast growth factor receptor 1 (FGFR1) point mutations are key molecular alterations in Pas, but their clinical implications are not established. We aimed to determine the frequency and prognostic role of these alterations in a cohort of 69 patients with PAs. We assessed KIAA1549:BRAF fusion by fluorescence in situ hybridization and BRAF (exon 15) mutations by capillary sequencing. In addition, FGFR1 expression was analyzed using immunohistochemistry, and this was compared with gene amplification and hotspot mutations (exons 12 and 14) assessed by fluorescence in situ hybridization and capillary sequencing. KIAA1549:BRAF fusion was identified in almost 60% of cases. Two tumors harbored mutated BRAF. Despite high FGFR1 expression overall, no cases had FGFR1 amplifications. Three cases harbored a FGFR1 p.K656E point mutation. No correlation was observed between BRAF and FGFR1 alterations. The cases were predominantly pediatric (87%), and no statistical differences were observed in molecular alterations-related patient ages. In summary, we confirmed the high frequency of KIAA1549:BRAF fusion in PAs and its association with a better outcome. Oncogenic mutations of FGFR1, although rare, occurred in a subset of patients with worse outcome. These molecular alterations may constitute alternative targets for novel clinical approaches, when radical surgical resection is unachievable.This study was partially supported by CNPq/Universal (475358/2011-2), and FAPESP (2012/19590-0) grants to RMR and to the NIH- P30CA046934 (CCSG Molecular Pathology/Cytogenetics) to MVG and DL

MET increased gene copy number and primary resistance to gefitinib therapy in non-small-cell lung cancer patients

Background: MET amplification has been detected in ∼20% of non-small-cell lung cancer patients (NSCLC) with epidermal growth factor receptor (EGFR) mutations progressing after an initial response to tyrosine kinase inhibitor (TKI) therapy. Patients and methods: We analyzed MET gene copy number using FISH in two related NSCLC cell lines, one sensitive (HCC827) and one resistant (HCC827 GR6) to gefitinib therapy and in two different NSCLC patient populations: 24 never smokers or EGFR FISH-positive patients treated with gefitinib (ONCOBELL cohort) and 182 surgically resected NSCLC not exposed to anti-EGFR agents. Results: HCC827 GR6-resistant cell line displayed MET amplification, with a mean MET copy number >12, while sensitive HCC827 cell line had a mean MET copy number of 4. In the ONCOBELL cohort, no patient had gene amplification and MET gene copy number was not associated with outcome to gefitinib therapy. Among the surgically resected patients, MET was amplified in 12 cases (7.3%) and only four (2.4%) had a higher MET copy number than the resistant HCC827 GR6 cell line. Conclusions: MET gene amplification is a rare event in patients with advanced NSCLC. The development of anti-MET therapeutic strategies should be focused on patients with acquired EGFR-TKI resistanc

Investigating the association between children who witness domestic volence in their household and being clinically diagnosed with childhood depression

Introduction/Objective: Depression is the most common psychiatric disorder in the general population. In particular, childhood depression is undertreated. Domestic violence affects more than 32 million Americans, and children who witness violence are more likely to display internalizing behaviors. Although the association between childhood abuse and depression in adulthood has been established, the objective of our study was to determine the association between children witnessing violence at home and being diagnosed with childhood depression. Methods: We used the 2011 NSCH database, which randomly surveyed parents in the U.S. Our cross-sectional study sample was limited to children 8-17 years old, and whose parents responded to specific survey questions. The exposure was children who witnessed domestic violence at home and the outcome was the diagnosis of childhood depression. Data analysis included 1) a descriptive analysis of population baseline characteristics, 2) a bivariate analysis of the baseline characteristics with the exposure and outcome, and 3) a multivariate logistic regression to control for potential confounders. Results: Our sample included 54,268 children. The unadjusted binary logistic regression indicated that children who witnessed domestic violence were 4.5 times more likely (95% CI 3.6- 5.5, p <0.001) to be diagnosed with childhood depression. However, the adjusted analysis did not find an association between witnessing domestic violence and childhood depression (OR 1.0, 95% CI 0.6-1.4, p: 0.834). Other variables independently associated with childhood depression include, but are not limited to: children whose school contacted the household about problems more than 10 times (OR 8.4, 95% CI 5.9-12.1, p: <0.001), poor general health of the child (OR 7.7, 95% CI 2.6-22.6, p <0.001), and poor mental health of the mother (OR 7.4, 95% CI 3.0-18.5, p <0.001). Conclusions: Our study found that children witnessing domestic violence do not have an increased risk of childhood depression. We found other factors such as problems at school or instability at home had a significant influence on whether a child was clinically depressed. Further research should be conducted to explore the associations of these other factors with childhood depression

Optimizing Yield and Quality of Orchardgrass Pasture in Temperate Silvipastoral Systems

The objective of this study was to provide grazing management recommendations for a silvipastoral system by determining the effects of shade and nitrogen (N) on the pattern of dry matter accumulation and nutritive value of orchardgrass (Dactylis glomerata) in a subhumid temperate environment. Orchardgrass in open pasture (100% transmittance) and under 10 year old Pinus radiata tree shade (60% transmittance) at 200 stems/ha was used as the main plot and nitrogen (0 and 300 kg N/ha as synthetic urine) was the subplot factor. Dry matter (DM) production of orchardgrass in the first 60-day spring rotation was similar in open and shade conditions (2.6 t/ha) but approximately doubled by the application of N. In the 60- day summer rotation, DM production was about 22% lower in the shaded plots and was increased by about 60% by the addition of N. Crude protein and organic matter digestibility declined with herbage age and the onset of reproductive tiller growth. It was concluded that to maximise DM production without compromising pasture quality, grazing management of orchardgrass should be similar in open and shaded pastures in spring (30-35 day regrowth) but a shorter regrowth length used for open (20 days) than silvipastoral (25-30 days) systems in summer

Dry Matter Production and Nutritive Value of Alfalfa (\u3ci\u3eMedicago sativa\u3c/i\u3e L.) and Orchardgrass (\u3ci\u3eDactylis glomerata\u3c/i\u3e L.) under Different Light Regimes

Spring and summer alfalfa dry matter and nitrogen production rates were greater than orchardgrass in open pasture and under three shade levels. Ten years old Pinus radiata allowed 60% light transmissivity. The addition of artificial shade resulted in 45% and 25% transmissivity. Orchardgrass pastures were nitrogen deficient and water stressed. In contrast, alfalfa was not stressed and it was more light responsive than orchardgrass. Differences in nutritive value were relatively small between species and between light regimes. Sheep liveweight gain on alfalfa was greater than on orchardgrass. Orchardgrass demonstrated shade tolerance, but alfalfa was more productive even at 25% transmissivity. Conventional concepts of shade tolerance may not be appropriate when screening pasture species for silvipastoral systems