50 research outputs found

    Streambank Erosion Associated with Grazing Practices in the Humid Region

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    The effects of cattle grazing on stream stability have been well documented for the western portion of the U.S., but are lacking for the east. Stream and riparian damage resulting from grazing can include alterations in watershed hydrology, changes to stream morphology, soil compaction and erosion, destruction of vegetation, and water quality impairments. However, few studies have examined the successes of best management practices (BMPs) for mitigating these effects. The objective of this project was to assess the ability of two common BMPs to reduce streambank erosion along a central Kentucky stream. The project site consisted of two replications of three treatments: (1) an alternate water source and a fenced riparian area to exclude cattle from the stream except at a 3.7 m wide stream ford, (2) an alternate water source with free stream access, and (3) free stream access without an alternate water source (i.e., control). Fifty permanent cross-sections were established throughout the project site. Each cross-section was surveyed monthly from April 2002 until November 2003. Results from the project indicated that the incorporation of an alternate water source and/or fenced riparian area did not significantly alter stream cross-sectional area over the treatment reaches. Rather than exhibiting a global effect, cattle activity resulted in streambank erosion in localized areas. As for the riparian exclosures, changes in cross-sectional area varied by location, indicating that localized site differences influenced the processes of aggradation and/or erosion. Hence, riparian recovery within the exclosures from pretreatment grazing practices may require decades, or even intervention (i.e., stream restoration), before a substantial reduction in streambank erosion is noted

    Contractile Response of Bovine Lateral Saphenous Vein to Ergotamine Tartrate Exposed to Different Concentrations of Molecularly Imprinted Polymer

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    Ergot alkaloids, in their active isomeric form, affect animal health and performance, and adsorbents are used to mitigate toxicities by reducing bioavailability. Adsorbents with high specificity (molecularly imprinted polymers: MIP) adsorb ergot alkaloids in vitro, but require evaluation for biological implications. Using ex vivo myography, synthetic polymers were evaluated for effects on the bioactivity of ergotamine tartrate (ETA). Polymers were first evaluated using isotherms. Lateral saphenous veins were collected from 17 steers for four independent studies: dose response of ETA, adsorbent dose response, validation of pre-myograph incubation conditions and MIP/ non-molecularly imprinted polymer (NIP) comparison. Norepinephrine normalized percent contractile response to increasing ETA exhibited a sigmoidal dose response (max: 88.47 and log of the effective molar concentration (EC50) (−log [ETA]) of 6.66 ± 0.17 M). Although sample preparation time affected contractile response (p \u3c 0.001), pre-myograph incubation temperature (39 vs. 21 °C, 1 h) had no effect (p \u3e 0.05). Isothermal adsorption showed a maximum adsorption of 3.27E-008 moles·mg−1 and affinity between 0.51 and 0.57 mg (R2: 0.83–0.92) for both polymers, with no significant difference between polymers (p \u3e 0.05). No significant differences in maximum inhibitory (p = 0.96) and IC50 responses (p = 0.163) between MIP and NIP were noticed. Normalized percent contraction could be predicted from the in vitro adsorption data (R2 = 0.87, p\u3c 0.01), for both polymers. These studies indicate that synthetic polymers are potentially effective adsorbents to mitigate ergot toxicity caused by ergot alkaloids, with little evidence of significant differences between MIP and NIP in aqueous media

    Yearling Beef Cattle Grazing Diverse Summer Annual Swards

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    Utilizing summer annuals is often regarded as “a breakeven proposition at best” due to high establishment costs. This study investigated using botanical diversity to increase forage yield or animal performance to improve the economic feasibility of grazing summer annual forages in western Kentucky, USA. Sorghum-sudangrass (Sorghum bicolor x S. bicolor spp. drummondii)-based treatments included a monoculture, a three-species mixture (two grasses, one legume), and a 12-species mixture (five grasses, four legumes, two brassicas, and one forb). Angus-cross yearling beef calves (329, 366, and 297 kg in 2017, 2018, and 2019, respectively) grazed summer annuals in 2017-2019 for an average of 40 days each year without supplementation. Pastures were stocked when forage was approximately 1 m tall and calves were removed when forage was 2-2.5 m tall (seedheads present). In all years, forage dry matter yield was not different between treatments (p \u3e 0.85) and both mixtures were dominated by sorghum-sudangrass. In 2017 (p \u3c 0.03) and 2019 (p \u3c 0.03), calves grazing the 12-species mixture had lower average daily gains (ADG) than the monoculture and 3-species mixture, while there was no difference in 2018 (p \u3e 0.3). Average daily gains were suboptimal for stocker calves in all years (0.75, 0.01, 0.54 kg day-1 in 2017, 2018, and 2019). The extremely low ADG in 2018 was likely a result of stocking pastures late in the season when grasses were at physiological maturity. Additional species increased seed cost but did not contribute significantly to forage production and did not result in increased animal production. Unless greater forage yield or livestock gains are attained, planting mixtures may not provide any economic benefit. However, adjusting seeding rates to favor less dominant species may provide a more accurate representation of species diversity manipulation effects on forage and livestock production

    Feeding Cannabidiol (CBD)-Containing Treats Did Not Affect Canine Daily Voluntary Activity

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    Growing public interest in the use of cannabidiol (CBD) for companion animals has amplified the need to elucidate potential impacts. The purpose of this investigation was to determine the influence of CBD on the daily activity of adult dogs. Twenty-four dogs (18.0 ± 3.4 kg, 9 months−4 years old) of various mixed breeds were utilized in a randomized complete block design with treatments targeted at 0 and 2.5 mg (LOW) and at 5.0 mg (HIGH) CBD/kg body weight (BW) per day split between two treats administered after twice-daily exercise (0700–0900 and 1,700–1,900 h). Four hours each day [1,000–1,200 h (a.m.) and 1,330–1,530 h (p.m.)] were designated as times when no people entered the kennels, with 2 h designated as Quiet time and the other 2 h as Music time, when calming music played over speakers. Quiet and Music sessions were randomly allotted to daily a.m. or p.m. times. Activity monitors were fitted to dogs\u27 collars for continuous collection of activity data. Data were collected over a 14-day baseline period to establish the activity patterns and block dogs by activity level (high or low) before randomly assigning dogs within each block to treatments. After 7 days of treatment acclimation, activity data were collected for 14 days. Data were examined for differences using the MIXED procedure in SAS including effects of treatment, day, session (Quiet or Music), time of day (a.m. or p.m.), and accompanying interactions. CBD (LOW and HIGH) did not alter the total daily activity points (P = 0.985) or activity duration (P = 0.882). CBD tended (P = 0.071) to reduce total daily scratching compared with the control. Dogs were more active in p.m. sessions than in a.m. sessions (P \u3c 0.001). During the p.m. session, dogs receiving HIGH tended (P = 0.091) to be less active than the control (CON). During the a.m. and p.m. sessions, CBD reduced scratching compared with CON (P = 0.030). CBD did not affect the activity duration during exercise periods (P = 0.143). These results indicate that, when supplemented with up to 4.5 mg CBD/kg BW/day, CBD does not impact the daily activity of adult dogs, but may exert an antipruritic effect

    Consumption of endophyte-infected fescue seed during the dry period does not decrease milk production in the following lactation.

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    Ergot alkaloids in endophyte-infected grasses inhibit prolactin (PRL) secretion and may reduce milk production of cows consuming these grasses. We investigated the effects of consuming endophyte-infected fescue seed during late lactation and the dry period on mammary growth, differentiation, and milk production. Twenty-four multiparous Holstein cows were randomly assigned to 3 treatment groups. Starting at 90±4 d prepartum, cows were fed endophyte-free fescue seed (control; CON), endophyte-free fescue seed plus 3×/wk subcutaneous injections of bromocriptine (0.1mg/kg of body weight, positive control; BROMO), or endophyte-infected fescue seed (INF) as 10% of the diet on an as fed basis. Although milk yield of groups did not differ before treatment, at dry off (-60 d prepartum) INF and BROMO cows produced less milk than CON. Throughout the treatment period, basal concentrations of PRL and the prepartum increase in plasma PRL were reduced in INF and BROMO cows compared with CON cows. Three weeks after the end of treatment, circulating concentrations of PRL were equivalent across groups. In the subsequent lactation milk yield was not decreased; in fact, BROMO cows exhibited a 9% increase in milk yield relative to CON. Evaluation of mammary tissue during the dry period and the subsequent lactation, by quantitative histology and immunohistochemical analysis of proliferation markers and putative mammary stem or progenitor cell markers, indicated that feeding endophyte-infected fescue seed did not significantly affect mammary growth and development. Feeding endophyte-infected grasses during the dry period may permit effective utilization of feed resources without compromising milk production in the next lactation

    Contractile Response of Bovine Lateral Saphenous Vein to Ergotamine Tartrate Exposed to Different Concentrations of Molecularly Imprinted Polymer

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    Ergot alkaloids, in their active isomeric form, affect animal health and performance, and adsorbents are used to mitigate toxicities by reducing bioavailability. Adsorbents with high specificity (molecularly imprinted polymers: MIP) adsorb ergot alkaloids in vitro, but require evaluation for biological implications. Using ex vivo myography, synthetic polymers were evaluated for effects on the bioactivity of ergotamine tartrate (ETA). Polymers were first evaluated using isotherms. Lateral saphenous veins were collected from 17 steers for four independent studies: dose response of ETA, adsorbent dose response, validation of pre-myograph incubation conditions and MIP/ non-molecularly imprinted polymer (NIP) comparison. Norepinephrine normalized percent contractile response to increasing ETA exhibited a sigmoidal dose response (max: 88.47 and log of the effective molar concentration (EC50) (−log [ETA]) of 6.66 ± 0.17 M). Although sample preparation time affected contractile response (p < 0.001), pre-myograph incubation temperature (39 vs. 21 °C, 1 h) had no effect (p > 0.05). Isothermal adsorption showed a maximum adsorption of 3.27E-008 moles·mg−1 and affinity between 0.51 and 0.57 mg (R2: 0.83–0.92) for both polymers, with no significant difference between polymers (p > 0.05). No significant differences in maximum inhibitory (p = 0.96) and IC50 responses (p = 0.163) between MIP and NIP were noticed. Normalized percent contraction could be predicted from the in vitro adsorption data (R2 = 0.87, p < 0.01), for both polymers. These studies indicate that synthetic polymers are potentially effective adsorbents to mitigate ergot toxicity caused by ergot alkaloids, with little evidence of significant differences between MIP and NIP in aqueous media
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