6 research outputs found

    Characterization of primary cultured MSCs.

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    <p>A: <i>In vitro</i> characterization of MSCs consisting of the specific surface marker antigen panel measured by flow cytometry. B: The differentiation capacity of MSCs towards osteoblasts shown by alkaline phosphatase activity and towards adipocytes shown by lipid droplets staining via Oil Red O. C: The inhibitory capacity of proliferation of activated peripheral blood mononuclear cells measured by 3H-thymidine uptake in counts per minute (CCPM). D: Bright light and fluorescence microscopy and flow cytometric analysis of eGFP labeling of lentivirally transduced MSCs.</p

    Stimulation of MSCs with the pro-inflammatory cytokine interferon gamma (IFN).

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    <p>A: From left to right: Immunostaining of iMSCs for HLA-DR in red (PE) and nuclei in blue (DAPI) and expression of HLA-DR as measured by flow cytometry. B: Immunostaining of iMSCs for the immunomodulatory enzyme IDO in green (ALEXA 488 nm) and nuclei in blue (DAPI).</p

    Cardiac function as assessed by 7 T MRI at 2 and 14 days after myocardial infarction in uMSC and iMSC treated animals (uMSC and iMSC, resp.), PBS treated animals (PBS) and Sham-operated animals (Sham) (panel A-C).

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    <p>A: Left ventricular ejection fraction. B: End-diastolic volume. C: End-systolic volume. Data are expressed as mean ± SD. # = p<0.05 versus Sham. D: Pressure Volume loops of all treatment groups at day 15 after MI. The oblique lines represent the end-systolic (Ees) and end-diastolic (Eed) pressure–volume relations.</p

    Assessment of engraftment of injected MSCs in mouse hearts 15 days after injection into the infarcted myocardium.

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    <p>A: Immunofluorescent staining of engrafted eGFP-labeled MSCs in red (Qdot 655) and nuclei in blue (hoechst 33342). B: Histological quantification of uMSC and iMSC engraftment (uMSC and iMSC resp.). PBS treated animals (PBS) and Sham-operated animals (Sham) were used as controls. C: Quantitative PCR for human genomic DNA in mouse hearts in animals treated with uMSC, iMSC, PBS and sham-operated animals. Data are expressed as mean ± SD. There were no significant differences between the uMSC and iMSC group.</p
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