Influence of a hypoiodite mouth-wash on dental plaque formation in vivo

This study describes an in vivo inhibition of dental plaque growth after peroxidase-generated hypoiodite (OI-) mouth-washes. After giving up ail other usual hygiene procedures nine healthy volunteers washed their mouth using 10 ml of the mouth-wash [H2O2 (0.005%), Kl (50 mM) and lactoperoxidase (0.04%)] three times a day for 1 minute for 3 days. The initial oxidation power of this mixture represented 430 ±11 µM oxidised cysteine (n=6), dropping down to 87 ± 6 µM after the solution was spat out (n=5). A saline solution served as a negative control, and a 0.2% chlorhexidine digluconate solution as a positive control. Proximal dental plaque between mandibular canine and lateral incisor (left and right) was collected after 3 days using standardized sterile toothpicks, then analysed for ATP and protein content. ATP concentrations dropped to 49% of the control values after OI- rinsing, and to 9% after chlorhexidine rinsing while the protein content dropped to 48% for OI- versus 31 % for chlorhexidine. However, when considering the ATP content per protein µg, only the decrease to 6% of the initial value in the chlorhexidine testing was significant while the drop to 81% for the OI- testings was not significant. This study points out a negative effect of OI- on plaque growth in vivo.Cette étude décrit l’inhibition de la croissance in vivo de la plaque dentaire après traitement à l’aide d’un bain de bouche contenant de la peroxydase et produisant de l’hypoiodite (OI-). Neuf personnes ont utilisé ce rinçage pendant une minute, 3 fois par jour pendant 3 jours, cependant qu’elles cessaient toute autre pratique d’hygiène bucco-dentaire; une solution saline servant de contrôle négatif et une solution de chlorhexidine de contrôle positif. Des échantillons de plaque interproximale furent prélevés de manière standardisée à l’aide de cure-dents stériles et leur contenu en ATP et en protéines furent mesurés. Les concentrations en ATP après traitement à l’OI- ne représentaient plus que 49% des valeurs des contrôles négatifs; le traitement à la chlorhexidine 31%. Le rapport ATP/masse protéique est fortement abaissé après traitement à la chlorhexidine (6%) mais se maintient à 85% de la valeur témoin après traitement à l’OI-

Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic

Our aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (=0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis.We thank Dr David Williams, Cardiff University, Cardiff, UK, for providing the strain 324LA/94. The authors also thank Sao Paulo Research Foundation (FAPESP, process 2009/15146-5), Brazil, for supporting the work of D. R. M. The authors are indebted to Laboratorio Interdisciplinar de Eletroquimica e Ceramica, Federal University of Sao Carlos, Brazil, in the name of Andressa Kubo, for preparing and characterizing the colloidal suspensions of silver nanoparticles

Dentisterie conservatrice de l'enfant, comprenant l'application clinique des matériaux

SYL-6463 = Pédodontie2e licence science dentaire - DENT 034info:eu-repo/semantics/published

Etude quantitative de la peroxydase salivaire chez l'homme

info:eu-repo/semantics/publishe

Traumatismes dentaires

info:eu-repo/semantics/publishe

Mesure, fonctions et utilisation des peroxydases dans la cavité buccale

Doctorat en sciences médicalesinfo:eu-repo/semantics/nonPublishe

Scale-independent CFD modeling of gas holdup and liquid velocity in bubble columns

Chemical Engineerin

Sealing ability of new generation adhesive systems in primary teeth: an in vitro study.

This study evaluated the sealing ability of different types of restorative-adhesive combinations on deciduous molars in vitro.Comparative StudyIn VitroJournal Articleinfo:eu-repo/semantics/publishe

Bond strengths of eight contemporary adhesives to enamel and to dentine: an in vitro study on bovine primary teeth.

The aim of this study was to compare the shear and tensile bond strengths of eight adhesive systems to the enamel and dentine of primary bovine teeth.Comparative StudyJournal ArticleFLWINinfo:eu-repo/semantics/publishe

Utilisation du mélange équimolaire oxygène/protoxyde d'azote (MEOPA) en dentisterie pédiatrique

SCOPUS: re.jinfo:eu-repo/semantics/publishe