Spectral-selective laser autofluorescent polarimetry of optically anisotropic structures of the myocardium in postmortem diagnosis of acute coronary insufficiency

Investigations of 69 samples of the myocardium following acute coronary insufficiency (ACI), 69 with chronic ischemic heart disease and 20 specimens of the control group were carried out. Operative characteristics of spectral-selective laser autofluorescent polarimetry of optically anisotropic structures of myocardium in comparison with the traditional method of ACI verification have been stated. The investigated method emonstrated a high level of the balanced accuracy for ACI diagnostic

Muller-matrix mapping of optically anisotropic molecular endogenic fluorophors of the myocardium in the diagnostics of acute coronary insufficiency

Investigation of 69 samples of the myocardium following acute coronary insufficiency (ACI) was carried out: 69 - after chronic ischemic heart disease and 20 - of the control group was. Operative characteristics of Muller-matrix mapping of optically anisotropic molecular endogenic fluorophors of the myocardium in comparison with the traditional method of ACI verification have been established. The method under investigation showed a good level of the balanced accuracy for the diagnostics of ACI

Acute coronary insufficiency postmortem diagnosis by the method of spectralselective laser autofluorescent polarimetry of myocardium

Diagnostics of acute coronary insufficiency (ACI) in the period till 6 hours from its beginning is an important issue of forensic medical practice. Combination of autofluorescent analysis with Muller-matrix one has been shown to be efficient method of ACI diagnostics. Aim of the research: to investigate spectral-selective laser autofluorescent polarimetry (SSLAP) of myocardium optically anisotropic structures possibilities for postmortem ACI diagnostics

Comparative analysis of laser polarimetry methods of polycrystalline films of cerebrospinal fluid for post-mortem interval estimation

Obtaining the most accurate information about an object during its evaluation is one of the most urgent tasks of current forensic medical examination. That is why the application of new technologies in forensic medical expertise becomes an integral part of the technological support of forensic examination institutions. Purpose: To compare the effectiveness of cerebrospinal fluid polycrystalline films, microscopic images, two-dimensional Stokes-polarimetry, and autofluorescent polarimetry in post-mortem interval estimation over long- and short-term time intervals. Material and methods. The object of the study is cerebrospinal fluid donated from 70 corpses (experimental group) and 20 living persons (control group). Methods used: two-dimensional Stokes-polarimetry, autofluorescent polarimetry. Results: Quantitative characteristics of the cerebrospinal fluid’s polycrystalline films statistical moments allows us to establish relations between them and the duration of the post-mortem interval at a significant time interval. However, this method does not consider the morphology of the film of the cerebrospinal fluid and its coordinate structure. In contrast to this method, fluorescence microscopy considers the biochemical composition of the biological fluid and carries information about the concentration of molecular complexes of proteins, NADH, flavins, porphyrins, etc. In the early post-mortem period, changes in the cerebrospinal fluid begin precisely with changes in the concentration of biochemical compounds, and crystalline changes are secondary, so it can be argued that this method is most effective for diagnosing the progression of the time in the first hours after death. Conclusions: Fluorescent laser polarimetric methods allow accurate estimation of the postmortem interval during the first hours after death. While, the polarization methods is more effective during the long-term periods of time, but has less precision

Azimuthally invariant Mueller-matrix mapping of biological optically anisotropic network

A new technique of Mueller-matrix mapping of polycrystalline structure of histological sections of biological tissues is suggested. The algorithms of reconstruction of distribution of parameters of linear and circular dichroism of histological sections liver tissue of mice with different degrees of severity of diabetes are found. The interconnections between such distributions and parameters of linear and circular dichroism of liver of mice tissue histological sections are defined. The comparative investigations of coordinate distributions of parameters of amplitude anisotropy formed by Liver tissue with varying severity of diabetes (10 days and 24 days) are performed. The values and ranges of change of the statistical (moments of the 1st – 4th order) parameters of coordinate distributions of the value of linear and circular dichroism are defined. The objective criteria of cause of the degree of severity of the diabetes differentiation are determined

Using of method of multiparametric spectral-selective laser polarization autofluorescence of biological layers for postmortem diagnostics of acute ischemia of myocardium

Significant prevalence of acute coronary insufficiency (ACI) and its suddenness in the forensic practice gives rise to suspicion of police about violent nature of death. That is why objective precise methods for diagnosis of acute ischemia (AI) of the myocardium requires. However, ACI is difficult to diagnose because of the nonspecificity of macroscopic signs and the need for specific coloring methods and the role of the "human factor"

Time since death estimation by two-dimensional mapping of polarizating inhomogeneous images of cerebro-spinal fluid polycrystalline films

Considеrablе difeсultiеs ofaссurate timе sinsе dеath (TSD) еstimation arе rеlatеd to impaсt of large number of Ьoth еxternal and internal faсtors on posthumous proсеssеs developmеnt. There arе many nеw tесhniques have Ьeen rеsеarсh in reсеnt yеars for TSD estimation inсluding bioсhemiсal' speсtrophotometriсf,l uoresсеnt- hystoсhеmiсalm ethods etс. In our opinion optiсal diaрostiс mеthods are thе most perspetivе in this arеa

Post-mortem interval estimation by changes in the optical density of the cerebrospinal fluid with the time after death increasing

The ability to accurately determine when death has occurred allows more accurate and with high quality provide the investigation of crimes, so the definition of a postmortem interval (PMI) covers both medical and legal aspects

System of multifunctional Jones matrix tomography of phase anisotropy in diagnostics of endometriosis

The paper presents the results of Jones-matrix mapping of uterine wall histological sections with second-degree and third-degree endometriosis. The technique of experimental measurement of coordinate distributions of the modulus and phase values of Jones matrix elements is suggested. Within the statistical and cross-correlation approaches the modulus and phase maps of Jones matrix images of optically thin biological layers of polycrystalline films of plasma and cerebrospinal fluid are analyzed. A set of objective parameters (statistical and generalized correlation moments), which are the most sensitive to changes in the phase of anisotropy, associated with the features of polycrystalline structure of uterine wall histological sections with second-degree and third-degree endometriosis are determined

Stokes-correlometry of polarization-inhomogeneous objects

In the series of research works the possibility of polarimetry diagnostic of optically anisotropic biological layers is demonstrated. Separate place in such investigations occupy laser polarimetry of optically thin (nondepolarizing) layers of biological tissues and fluids. At that high sensitivity of laser polarimetry techniques is achieve