Validation of a method for measuring sperm quality and quantity in reproductive toxicity tests with pair-breeding male fathead minnows (Pimephales promelas)

This article originally appeared in the ILAR e-Journal. It is reprinted with permission from the ILAR Journal, Institute for Laboratory Animal Research, National Research Council, Washington DC (www.nationalacademies.org/ilar).The fathead minnow (Pimephales promelas) is an OECD-proposed test species routinely used in reproductive toxicity trials with suspected endocrine-disrupting compounds (EDCs). The basic fecundity, endocrinology, and histopathology of reproductively active male and female fathead minnows has been well characterized, but there are few studies of the utility of male sperm concentration and motility as endpoints for use in reproductive trials. The purpose of this study was to (1) characterize the baseline sperm concentration and motility of pair-breeding male fathead minnows over their spawning cycle and (2) determine whether a repeated and nondestructive sperm sampling protocol would influence the baseline fecundity of the fish. Pair-breeding male fathead minnows that underwent sampling for milt three times a week for 4 weeks exhibited no significant changes in milt volume, sperm concentration, or motility parameters up to 6 days after each spawning event. The repeated sperm sampling procedure did, however, cause a significant lowering of spawning frequencies, although this decline did not correlate with effects on fecundity as there were no significant changes in the mean total numbers of eggs laid, fertilization, and hatching successes. This study confirmed the presence of a stable background of sperm concentration and motility parameters of pair-breeding male fathead minnows under reference conditions. The absence of any inherent “cycling” in the magnitude of these parameters over the spawning period suggests that sperm concentration and motility could be useful measures of male reproductive toxicity at the termination of tests in which pair-breeding males are at varying days post spawn.The research described was funded by the EU project Comparative Research on Endocrine Disrupters (COMPRENDO) Institute of Zoology Regents Park, London, contract No. EVK1-CT-2002-00129E

Vortex configurations in a Pb/Cu microdot with a 2x2 antidot cluster

We present a detailed study of the transport properties of a superconducting Pb/Cu microdot with a 2x2 antidot cluster. The superconducting-normal (S/N) phase boundary, critical currents and current-voltage characteristics of this structure have been measured. The S/N phase boundary as a function of field B (T_c(B)) reveals an oscillatory structure caused by the limited number of possible vortex configurations which can be realized in these small clusters of pinning centres (antidots). We have analyzed the stability of these configurations and discussed the possible dissipation mechanisms using the critical current (J_c(B)) and voltage-current (V(I)) characteristics data. A comparison of the experimental data of T_c(B) and J_c(B) with calculations in the London limit of the Ginzburg-Landau theory confirms that vortices can indeed be pinned by the antidots forming a cluster and that the ground-state configurations of the vortices are noticeably modified by sending current through the structure. The possibility of generating phase-slips as well as motion of the vortices in the 2x2 antidot cluster has also been discussed.Comment: RevTeX, 22 pages, 15 figures, accepted for publication in PR

Confinement and Quantization Effects in Mesoscopic Superconducting Structures

We have studied quantization and confinement effects in nanostructured superconductors. Three different types of nanostructured samples were investigated: individual structures (line, loop, dot), 1-dimensional (1D) clusters of loops and 2D clusters of antidots, and finally large lattices of antidots. Hereby, a crossover from individual elementary "plaquettes", via clusters, to huge arrays of these elements, is realized. The main idea of our study was to vary the boundary conditions for confinement of the superconducting condensate by taking samples of different topology and, through that, modifying the lowest Landau level E_LLL(H). Since the critical temperature versus applied magnetic field T_c(H) is, in fact, E_LLL(H) measured in temperature units, it is varied as well when the sample topology is changed through nanostructuring. We demonstrate that in all studied nanostructured superconductors the shape of the T_c(H) phase boundary is determined by the confinement topology in a unique way.Comment: 28 pages, 19 EPS figures, uses LaTeX's aipproc.sty, contribution to Euroschool on "Superconductivity in Networks and Mesoscopic Systems", held in Siena, Italy (8-20 september 1997

Ratchet Cellular Automata

In this work we propose a ratchet effect which provides a general means of performing clocked logic operations on discrete particles, such as single electrons or vortices. The states are propagated through the device by the use of an applied AC drive. We numerically demonstrate that a complete logic architecture is realizable using this ratchet. We consider specific nanostructured superconducting geometries using superconducting materials under an applied magnetic field, with the positions of the individual vortices in samples acting as the logic states. These devices can be used as the building blocks for an alternative microelectronic architecture.Comment: 4 pages, 3 figure

Ratchet Effects for Vortices in Superconductors with Periodic Pinning Arrays

Using numerical simulations we show that novel transport phenomena can occur for vortices moving in periodic pinning arrays when two external perpendicular ac drives are applied. In particular, we find a ratchet effect where the vortices can have a net dc drift even in the absence of a dc drive. This ratchet effect can occur for ac drives which create orbits that break one or more reflection symmetries.Comment: 4 pages, 4 postscript figures; Proceedings of Third European Conference on Vortex Matter in Superconductor

Hall carrier density and magnetoresistance measurements in thin film vanadium dioxide across the metal-insulator transition

Temperature dependent magneto-transport measurements in magnetic fields of up to 12 Tesla were performed on thin film vanadium dioxide (VO2) across the metal-insulator transition (MIT). The Hall carrier density increases by 4 orders of magnitude at the MIT and accounts almost entirely for the resistance change. The Hall mobility varies little across the MIT and remains low, ~0.1cm2/V sec. Electrons are found to be the major carriers on both sides of the MIT. Small positive magnetoresistance in the semiconducting phase is measured

Development of an oximeter for neurology

Cerebral desaturation can occur during surgery manipulation, whereas otherparameters vary insignificantly. Prolonged intervals of cerebral anoxia can cause seriousdamage to the nervous system. Commonly used method for measurement of cerebral bloodflow uses invasive catheters. Other techniques include single photon emission computedtomography (SPECT), positron emission tomography (PET), magnetic resonance imaging(MRI). Tomographic methods frequently use isotope administration, that may result inanaphylactic reactions to contrast media and associated nerve diseases. Moreover, the high costand the need for continuous monitoring make it difficult to apply these techniques in clinicalpractice. Cerebral oximetry is a method for measuring oxygen saturation using infraredspectrometry. Moreover reflection pulse oximetry can detect sudden changes in sympathetictone. For this purpose the reflectance pulse oximeter for use in neurology is developed.Reflectance oximeter has a definite advantage as it can be used to measure oxygen saturation inany part of the body. Preliminary results indicate that the device has a good resolution and highreliability. Modern applied schematics have improved device characteristics compared withexisting ones