Nictaba homologs from Arabidopsis thaliana are involved in plant stress responses

Plants are constantly exposed to a wide range of environmental stresses, but evolved complicated adaptive and defense mechanisms which allow them to survive in unfavorable conditions. These mechanisms protect and defend plants by using different immune receptors located either at the cell surface or in the cytoplasmic compartment. Lectins or carbohydrate-binding proteins are widespread in the plant kingdom and constitute an important part of these immune receptors. In the past years, lectin research has focused on the stress-inducible lectins. The Nicotiana tabacum agglutinin, abbreviated as Nictaba, served as a model for one family of stress-related lectins. Here we focus on three non-chimeric Nictaba homologs from Arabidopsis thaliana, referred to as AN3, AN4, and AN5. Confocal microscopy of ArathNictaba enhanced green fluorescent protein (EGFP) fusion constructs transiently expressed in N. benthamiana or stably expressed in A. thaliana yielded fluorescence for AN4 and AN5 in the nucleus and the cytoplasm of the plant cell, while fluorescence for AN3 was only detected in the cytoplasm. RT-qPCR analysis revealed low expression for all three ArathNictabas in different tissues throughout plant development. Stress application altered the expression levels, but all three ArathNictabas showed a different expression pattern. Pseudomonas syringae infection experiments with AN4 and AN5 overexpression lines demonstrated a significantly higher tolerance of several transgenic lines to P. syringae compared to wild type plants. Finally, AN4 was shown to interact with two enzymes involved in plant defense, namely TGG1 and BGLU23. Taken together, our data suggest that the ArathNictabas represent stress-regulated proteins with a possible role in plant stress responses. On the long term this research can contribute to the development of more stress-resistant plants

Attentional bias towards pain-related information diminishes the efficacy of distraction

Distraction is a strategy that is commonly used to cope with pain. Results concerning the efficacy of distraction from both experimental and clinical studies are variable, however, and indicate that its efficacy may depend on particular circumstances. Several models propose that distraction may be less effective for people who display a large attentional bias towards pain-related information. This hypothesis was tested in an experimental context with 53 pain-free volunteers. First, attentional bias towards cues signalling the occurrence of pain (electrocutaneous stimuli) and towards words describing the sensory experience of this painful stimulus was independently assessed by means of 2 behavioural paradigms (respectively, spatial cueing task and dot-probe task). This was followed by a subsequent distraction task during which the efficacy of distraction, by directing attention away from the electrocutaneous stimuli, was tested. In addition, state-trait anxiety, catastrophic thinking, and initial pain intensity were measured. Results indicated that people who display a large attentional bias towards predictive cues of pain or who initially experience the pain as more painful benefit less from distraction on a subsequent test. No effects were found between attentional bias towards pain words, state-trait anxiety, catastrophic thinking, and the efficacy of distraction. Current findings suggest that distraction should not be used as a 'one size fits all' method to control pain, but only under more specific conditions

Psychiatric disorders and future violent arrests : a prospective study among detained girls

This study scrutinizes if detained girls with psychiatric disorders were at risk for future violent arrests during adolescence. A structured diagnostic interview was performed to determine the presence of various psychiatric disorders in 313 detained girls. Official juvenile arrest records were collected. With three exceptions that are in need of replication, psychiatric disorders (e.g., post-traumatic stress disorder, subtypes of conduct disorder), psychiatric disorder categories (e.g., anxiety disorders), and psychiatric comorbidity patterns were not prospectively related to future violent arrests. Our findings suggest that detained girls with psychiatric disorders should not be considered more dangerous than their counterparts without disorders, at least not during adolescence

Detection of tactile change detection on a bodily location where pain is expected

As it is adaptive to accurately detect and localize bodily threats, it has been proposed that the brain prioritizes somatosensory input at body locations where pain is expected. To test this proposition, the detection of tactile changes on a body location was investigated to assess whether detection was facilitated by threat of pain. Healthy participants (N = 47) indicated whether two consecutive patterns of three tactile stimuli were the same or not. Stimuli could be administered at eight possible locations. In half of the trials, the same pattern was presented twice. In the other half, one stimulus location was different between the two displays. To induce bodily threat, a painful stimulus was occasionally administered to the non-dominant lower arm. Mean accuracy of tactile change detection as a function of location was analyzed using repeated-measures analysis of variance (ANOVA). Tactile changes on the threatened arm (i.e., when a tactile stimulus emerged at or disappeared from that arm), both at the exact pain location (lower arm) and at the other location (upper arm), were better detected than tactile changes on other limbs

The bumpy road to change : a retrospective qualitative study on formerly detained adolescents' trajectories towards better lives

Background: Currently, the risk-oriented focus in forensic youth care is increasingly complemented by a growing interest in strengths-based approaches. Knowledge on how detention and the subsequent period in the community is experienced by adolescents, and which elements are helpful in achieving better lives can contribute to this emerging field. The current study aimed to retrospectively explore adolescents’ experiences from the moment they were detained until six to 12 months after they left the institution, identifying positive aspects and strengths. Methods: In-depth interviews were conducted with 25 adolescents (both boys and girls, 15-18 years old) on average eight months after discharge from a closed institution in Belgium. A thematic analysis was performed using NVivo 11. Results: Five themes and corresponding subthemes were identified: (1) adolescents’ own strengths and resilience, (2) re-building personally valued lives, (3) making sense of past experiences, (4) moving away from a harmful lifestyle, and (5) (in-)formal supports. Most adolescents are on their way to finding a new balance in life, however, for a subgroup of them, this is still fragile. Adolescents highly emphasize the importance of feeling closely connected to at least one person; to receive practical help with regard to finances, work and housing; and to be able to experience pleasure and joy in their lives. Conclusions: Adolescents’ narratives suggest that starting a journey towards a normative good life often goes along with an initial difficult period because of a sense of loss with regard to their former life. This stresses the importance of targeting rehabilitation towards prosocial goals and enhancing adolescents’ quality of life on those life domains that matter most for them. Furthermore, we stress the importance of helping adolescents in overcoming structural barriers as a first step in supporting them in their trajectories towards better lives