17 research outputs found
Preimplantation Mouse Embryo Selection Guided by Light-Induced Dielectrophoresis
Selection of optimal quality embryos for in vitro fertilization (IVF) transfer is critical to successful live birth outcomes. Currently, embryos are chosen based on subjective assessment of morphologic developmental maturity. A non-invasive means to quantitatively measure an embryo's developmental maturity would reduce the variability introduced by the current standard. We present a method that exploits the scaling electrical properties of pre-transfer embryos to quantitatively discern embryo developmental maturity using light-induced dielectrophoresis (DEP). We show that an embryo's DEP response is highly correlated with its developmental stage. Uniquely, this technique allows one to select, in sequence and under blinded conditions, the most developmentally mature embryos among a mixed cohort of morphologically indistinguishable embryos cultured in optimized and sub-optimal culture media. Following assay, embryos continue to develop normally in vitro. Light-induced dielectrophoresis provides a non-invasive, quantitative, and reproducible means to select embryos for applications including IVF transfer and embryonic stem cell harvest
Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate
The authors demonstrate an optical manipulation mechanism of gas bubbles for microfluidic applications. Air bubbles in a silicone oil medium are manipulated via thermocapillary forces generated by the absorption of a laser in an amorphous silicon thin film. In contrast to previous demonstrations of optically controlled thermally driven bubble movement, transparent liquids can be used, as the thermal gradient is formed from laser absorption in the amorphous silicon substrate, and not in the liquid. A variety of bubbles with volumes ranging from 19 pl to 23 nl was transported at measured velocities of up to 1.5 mm/s
Parallel assembly of nanowires using lateral-field optoelectronic tweezers
We report on the parallel manipulation and assembly of nanowires using paired virtual optical tips projected on lateral-field optoelectronic tweezers. Precise position and angular control has been demonstrated on four 80-nm-diameter silver nanowires
In-situ single cell electroporation using optoelectronic tweezers
Optoelectronic Tweezers are used to achieve light-induced, in-situ electroporation of HeLa cells. By controlling electrical bias, patterned light induces either single cell movement or electroporation. Fluorescent dye and dielectrophoretic response are used to monitor electroporation
Parallel single-cell light-induced electroporation and dielectrophoretic manipulation
Electroporation is a common technique for the introduction of exogenous molecules across the, otherwise, impermeant cell membrane. Conventional techniques are limited by either low throughput or limited selectivity. Here we present a novel technique whereby we use patterned light to create virtual electrodes which can induce the parallel electroporation of single cells. This technique seamlessly integrates with optoelectronic tweezers to provide a single cell manipulation platform as well. We present evidence of parallel, single cell electroporation using this method through use of fluorescent dyes and dielectrophoretic responses. Additionally, through the use of integrated microfluidic channels, we show that cells remain viable following treatment in the device. Finally, we determine the optimal field dosage to inject propidium iodide into a HeLa cell and maintain cellular viability