<div>We have studied in vitro toxicity of iron oxide nanoparticles (NPs) coated with a thin silica shell (Fe3O4/SiO2 NPs) on A549 and HeLa cells. We compared bare and surface passivated Fe3O4/SiO2 NPs to evaluate the effects of the coating on the particle stability and toxicity. NPs cytotoxicity was investigated by cell viability, membrane integrity, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) assays, and their genotoxicity by comet assay. Our results show that NPs surface passivation reduces the oxidative stress and alteration of iron homeostasis and, consequently, the overall toxicity, despite bare and passivated NPs show similar cell internalization efficiency. We found that the higher toxicity of bare NPs is due to their stronger in-situ degradation, with larger intracellular release of iron ions, as compared to surface passivated NPs. Our results indicate that surface engineering of Fe3O4/SiO2 NPs plays a key role in improving particles stability in biological environments reducing both cytotoxic and genotoxic effects.</div

Antonio Galeone (189781)

Athanassia Athanassiou (511638)

George C. Anyfantis (511637)

Maria Ada Malvindi (257894)

Pier Paolo Pompa (189798)

Roberto Cingolani (189795)

Valeria De Matteis (511636)

Virgilio Brunetti (189786)

Directory of Open Access Journals

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A model of Lysosomal Metabolism of Dextran Coated Superparamagnetic Iron Oxide (SPIO) Nanoparticles: Implications for Cellular magnetic resonance imaging.

Abdominal MR: liver and pancreas.

Biochemically functionalized silica nanoparticles.

Concentrations and elemental composition of particulate matter in the Buenos Aires underground system.

Cytotoxic effects and the mechanism of three types of magnetic nanoparticles on human hepatoma BEL-7402 cells.

Cytotoxic effects of iron oxide nanoparticles and implications for safety in cell labelling.

Degradability of superparamagnetic nanoparticles in a model of intracellular environment: follow-up of magnetic, structural and chemical properties.

Degradation of superparamagnetic iron oxide nanoparticle-induced ferritin by lysosomal cathepsins and related immune response.

Effect of surface charge and agglomerate degree of magnetic iron oxide nanoparticles on KB cellular uptake in vitro.

Effect of the presence or absence of a protein corona on silica nanoparticle uptake and impact on cells.

Effects of cell culture media on the dynamic formation of protein-nanoparticle complexes and influence on the cellular response.

Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells.

Enhanced cellular uptake of aminosilanecoated superparamagnetic iron oxide nanoparticles in mammalian cell lines.

Ferucarbotran (Resovist): a new clinically approved RES-specific contrast agent for contrast-enhanced MRI of the liver—properties, clinical development, and applications.

High intracellular iron oxide nanoparticle concentrations affect cellular cytoskelet on and focal adhesion kinase-mediated signaling.

Inorganic nanoparticles for MRI contrast agents.

InP/ZnS as a safer alternative to CdSe/ZnS core/shell quantum dots: in vitro and in vivo toxicity assessment.

Iron chelators in medicinal applications - chemical equilibrium considerations in pharmaceutical activity.

Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling.

Ligand design and Bioconjugation of colloidal gold nanoparticles.

Lysosomal degradation of the carboxydextran shell of coated superparamagnetic iron oxide nanoparticles and the fate of professional phagocytes.

Magnetic iron oxide nanoparticles: synthesis and surface functionalization strategies.

Magnetic nanovectors for drug delivery.

Magnetic resonance imaging of transplanted mouse islets labeled with chitosan-coated superparamagnetic iron oxide nanoparticles.

Magnetic Silica Nanoparticle Cellular Uptake and Cytotoxicity regulated by Electrostatic Polyelectrolytes-DNA Loading at their Surface.

Magnetic/Silica Nanocomposites as Dual-Mode Contrast Agents for Combined Magnetic Resonance Imaging and Ultrasonography.

Magnetically enhanced nucleic acid delivery. Ten years of magnetofection-progress and prospects.

Optimizing magnetic nanoparticle design for nanothermotherapy.

Oxidations of iron(II)/(III) by hydrogen peroxide: from aquo to enzyme Coordination. Chem Rev 311–318.

Particle emissions of a railway line determined by detailed single particle analysis.

Petri-Fink A

Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION).

Preparation and characterization of chemically functionalized silica-coated magnetic nanoparticles as a DNA separator.

SiO2 nanoparticles biocompatibility and their potential for gene delivery and silencing.

Size controlled synthesis of dextran sulfate coated iron oxide nanoparticles for magnetic resonance imaging.

Stabilising fluorescent silica nanoparticles against dissolution effects for biological studies.

Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups.

Superparamagnetic iron oxide nanoparticles change endothelial cell morphology and mechanics via reactive oxygen species formation.

Superparamagnetic iron oxide nanoparticles: from preparations to in vivo MRI applications.

The biocompatibility of amino functionalized CdSe/ZnS quantum dot-doped SiO2 nanoparticles with primary neural cells and their gene carrying performance.

The biomolecular corona is retained during nanoparticle uptake and protects the cells from the damage induced by cationic nanoparticles until degraded in the lysosomes.

The challenge to relate the physicochemical properties of colloidal nanoparticles to their cytotoxicity.

The effect of an iron (III) chelator, silybin, on the proliferation and cell cycle of Jurkat cells: a comparison with desferrioxamine.

The inhibitory effect of superparamagnetic iron oxide nanoparticles (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells.

The role of iron redox state in the genotoxicity of ultrafine superparamagnetic iron oxide nanoparticles.

Toxicity evaluations of superparamagnetic iron oxide nanoparticles: cell &quot;vision&quot; versus physicochemical properties of nanoparticles.

Toxicity Assessment of Silica Coated Iron Oxide Nanoparticles and Biocompatibility Improvement by Surface Engineering