Duration of the process at Emergency-clinical Laboratory. “Leon Cuervo Rubio” General Hospital, Pinar del Rio

Introducción: el laboratorio clínico proporciona datos cualitativos y cuantitativos como ayuda a la prevención, diagnóstico y pronóstico de enfermedades humanas. Ello implica todo un conjunto de medidas encaminadas a lograr una adecuada confiabilidad de los resultados. Objetivo: Identificar los factores que inciden directamente sobre la calidad del servicio que brinda el laboratorio clínico de urgencia del Hospital Provincial Clínico Quirúrgico "León Cuervo Rubio" de Pinar del Río. Material y método: estudio prospectivo transversal donde el universo fueron los pacientes que usaron los servicios del laboratorio clínico de urgencia y muestra 500 pacientes a los que se les determinó el tiempo de trabajo o tiempo que demora la muestra en dos etapas. Primera Etapa: Se tomaron 250 pacientes que acudieron al laboratorio de urgencia, los que fueron atendidos por los equipos de trabajo habituales (cuatro en total), fueron clasificados en pacientes internos y en externos. Segunda Etapa: Se aplicó un plan de acción a partir de los resultados obtenidos en la primera etapa y se siguió igual esquema de trabajo a la etapa anterior. Resultados: el tiempo de trabajo en la primera etapa fue excesivamente prolongado, el informe de entrega demoró menos en los pacientes externos y existieron comportamientos diferentes entre los equipos que participaron. Conclusiones: pobre desempeño del personal de apoyo y mensajería que vinculan el laboratorio con el paciente. Diferente nivel de prioridad del laboratorio evidenciándose mediante la afirmación de que el Tiempo que demora la muestra es menor en pacientes externos, con relación a los pacientes ingresadosIntroduction: clinical laboratory provides qualitative and quantitative data as a method of prevention, diagnosis and prognosis of human being diseases. It implies a set of measures aimed at achieving suitable reliability of lab results. Objective: to identify the factors having directly influence on the quality of service at the emergency clinical laboratory, “Leon Cuervo Rubio” General Hospital, Pinar del Rio.Material and method: cross-sectional prospective study where the target group included the whole patients who used the services of the emergency- clinical laboratory and the sample involved 500 patients to whom the process time was determined or the time the specimen lasted in two stages. First stage: 250 patients attended to the emergency laboratory, they were seen by the habitual team-works (4 in total) and were classified into inpatients and outpatients. Second stage: an action plan was applied in view of the results obtained in the first stage and the same outline of work was carried out the same of the previous stage. Results: process time in the first stage was excessively prolonged, the final report lasted less for outpatients and different behaviors were observed among the team-works that participated.Conclusions: poor performance of support and message personnel concerning the laboratory with the patient was observed. Proving a different level of priority in the laboratory through the confirmation of the duration of the process, which it is less for outpatients than for inpatients

Tiempo de trabajo en el laboratorio clínico de urgencia del Hospital "León Cuervo Rubio". Pinar del Río

Introduction: clinical laboratory provides qualitative and quantitative data as a method of prevention, diagnosis and prognosis of human being diseases. It implies a set of measures aimed at achieving suitable reliability of lab results. Objective: to identify the factors having directly influence on the quality of service at the emergency clinical laboratory, “Leon Cuervo Rubio” General Hospital, Pinar del Rio.Material and method: cross-sectional prospective study where the target group included the whole patients who used the services of the emergency- clinical laboratory and the sample involved 500 patients to whom the process time was determined or the time the specimen lasted in two stages. First stage: 250 patients attended to the emergency laboratory, they were seen by the habitual team-works (4 in total) and were classified into inpatients and outpatients. Second stage: an action plan was applied in view of the results obtained in the first stage and the same outline of work was carried out the same of the previous stage. Results: process time in the first stage was excessively prolonged, the final report lasted less for outpatients and different behaviors were observed among the team-works that participated.Conclusions: poor performance of support and message personnel concerning the laboratory with the patient was observed. Proving a different level of priority in the laboratory through the confirmation of the duration of the process, which it is less for outpatients than for inpatients.Introducción: el laboratorio clínico proporciona datos cualitativos y cuantitativos como ayuda a la prevención, diagnóstico y pronóstico de enfermedades humanas. Ello implica todo un conjunto de medidas encaminadas a lograr una adecuada confiabilidad de los resultados. Objetivo: Identificar los factores que inciden directamente sobre la calidad del servicio que brinda el laboratorio clínico de urgencia del Hospital Provincial Clínico Quirúrgico "León Cuervo Rubio" de Pinar del Río. Material y método: estudio prospectivo transversal donde el universo fueron los pacientes que usaron los servicios del laboratorio clínico de urgencia y muestra 500 pacientes a los que se les determinó el tiempo de trabajo o tiempo que demora la muestra en dos etapas. Primera Etapa: Se tomaron 250 pacientes que acudieron al laboratorio de urgencia, los que fueron atendidos por los equipos de trabajo habituales (cuatro en total), fueron clasificados en pacientes internos y en externos. Segunda Etapa: Se aplicó un plan de acción a partir de los resultados obtenidos en la primera etapa y se siguió igual esquema de trabajo a la etapa anterior. Resultados: el tiempo de trabajo en la primera etapa fue excesivamente prolongado, el informe de entrega demoró menos en los pacientes externos y existieron comportamientos diferentes entre los equipos que participaron. Conclusiones: pobre desempeño del personal de apoyo y mensajería que vinculan el laboratorio con el paciente. Diferente nivel de prioridad del laboratorio evidenciándose mediante la afirmación de que el Tiempo que demora la muestra es menor en pacientes externos, con relación a los pacientes ingresado

Impacto ambiental del vial de distribución principal de Cayo Cruz, Camagüey / Environmental impacts of main distribution road of Cayo Cruz, Camagüey

The article aims to expose the results of the environmental impact assessment carried out to the main distribution road project in the tourist pole Cayo Cruz, Camagüey. A total of 32 impacts between positive and negative were identified. The facilitation of tourism development is the most important of the positive impacts. The potential negative impacts of greater range are biological / ecological, such as those related to the possible effects on species of special interest for biological diversity and can be mitigated with the implementation of a group of measures that are suggested in this work.El artículo persigue exponer los resultados de la evaluación de impacto ambiental realizada al proyecto del vial principal de distribución en el polo turístico Cayo Cruz, Camagüey. Se identificaron un total de 32 impactos entre positivos y negativos. La facilitación del desarrollo turístico es el más importante de los impactos positivos. Los impactos negativos potenciales de mayor rango son los biológicos/ecológicos, como los relacionados con las posibles afectaciones a las especies de especial interés para la diversidad biológica y pueden ser mitigados con la implementación de un grupo de medidas que son sugeridas en el presente trabajo. / The article aims to expose the results of the environmental impact assessment carried out to the main distribution road project in the tourist pole Cayo Cruz, Camagüey. A total of 32 impacts between positive and negative were identified. The facilitation of tourism development is the most important of the positive impacts. The potential negative impacts of greater range are biological / ecological, such as those related to the possible effects on species of special interest for biological diversity and can be mitigated with the implementation of a group of measures that are suggested in this work

Relationship between morphology and dispersion of Juglansjamaicensis DC with the distance to the water course

The study was conducted in the Turquino National Park in the Zonal Conservation Units Santo Domingo and La Platica, to establish the relationship between the shape of the seeds of Juglansjamaicensis and distance to waterways as an adaptive response to their dispersal mechanism 7 groups of trees were selected. Each player is taken tree seeds 50 which were performed measurements of height (H), width (L) and thickness (E) to determine the rate of roundness (R). The distance of each tree nearest the course of water was also measured. The relationship between the two variables was performed through a simple correlation analysis; however the comparison of means was through a simple ANOVA, while the distribution of the shape of the seeds in relation to the distance to the watercourse was analyzed through the Tukey test. Those trees closer to water courses had more round seeds, indicating that the mechanism of scattering by water influences the environment in which they live. The roundness index varied and seeds were identified with rates ranging from medium to high (0, 81 to 1, 12; mean = 1, 01). These results suggest that the shape of the seeds is an adaptation of trees J. jamaicensis the medium in response to dispersal mechanism for water

La ultrasonografía doppler-color en el diagnóstico de las malformaciones vasculares

Objective: to characterize the ultrasound findings in patients with the clinical diagnosis of cutaneous vascular malformations in patients treated in the consultation of the National Institute of Angiology and Vascular Surgery Methods: a descriptive, prospective and cross-sectional study was carried out in 33 patients older than one year of age who attend the imaging consultation for the ultrasonographic diagnosis of angiodysplasia, and those who underwent ultrasonographic follow-up. The variables age, sex, location, size, family history of these malformations and presence of complications were taken into account. Results: the angiodysplasias was more representative in the age group of 15-35 (51.5%), those classified as large, according to the posterior anterior diameter, presented 54.6%, predominantly arterial and venous angiodysplasias, with greater localization in the lower extremities (37.2%) and predominant as a symptom the increase in volume in the large (54.2%) and presenting as complications the AVF, takes muscle. Conclusions: Doppler-associated ultrasound provides, in addition to anatomical information, hemodynamic data such as velocity and flow direction, vessel type, complication and follow-up with a systematic way of writing the ultrasonographic report.Objetivo: caracterizar los hallazgos ecográficos en los pacientes con el diagnóstico clínico de malformaciones vasculares cutáneas en los pacientes atendidos en la consulta del Instituto Nacional de Angiología y Cirugía Vascular. Métodos: se realizó un estudio descriptivo, prospectivo y transversal en 33 pacientes mayores de un año que asisten a la consulta de imagenología para el diagnóstico ultrasonográfico de las angiodisplasias, y a los que se les realizó un seguimiento ultrasonográfico. Se tuvo en cuenta las variables edad, sexo, localización, tamaño, antecedentes en la familia de estas malformaciones y presencia de complicaciones. Resultados: las angiodisplasias fue más representativo en el grupo etareo de 15-35 (51,5%), las clasificadas como grande, según el diámetro antero posterior, presentaron un 54,6%, predominando las angiodisplasias arteriales y venosas, con mayor localización en las extremidades inferiores (37,2%) y predominando como síntoma el aumento de volumen en los grandes (54,2%) y presentando como complicaciones las FAV, toma muscular. Conclusiones: la ecografía asociada al Doppler proporciona, además de la información anatómica, los datos hemodinámicos como la velocidad y la dirección del flujo, el tipo de vaso, la complicación y su seguimiento con una forma sistemática de redacción del informe ultrasonográfico

Voltage-Activated Calcium Channels as Functional Markers of Mature Neurons in Human Olfactory Neuroepithelial Cells: Implications for the Study of Neurodevelopment in Neuropsychiatric Disorders

In adulthood, differentiation of precursor cells into neurons continues in several brain structures as well as in the olfactory neuroepithelium. Isolated precursors allow the study of the neurodevelopmental process in vitro. The aim of this work was to determine whether the expression of functional Voltage-Activated Ca2+ Channels (VACC) is dependent on the neurodevelopmental stage in neuronal cells obtained from the human olfactory epithelium of a single healthy donor. The presence of channel-forming proteins in Olfactory Sensory Neurons (OSN) was demonstrated by immunofluorescent labeling, and VACC functioning was assessed by microfluorometry and the patch-clamp technique. VACC were immunodetected only in OSN. Mature neurons responded to forskolin with a five-fold increase in Ca2+. By contrast, in precursor cells, a subtle response was observed. The involvement of VACC in the precursors’ response was discarded for the absence of transmembrane inward Ca2+ movement evoked by step depolarizations. Data suggest differential expression of VACC in neuronal cells depending on their developmental stage and also that the expression of these channels is acquired by OSN during maturation, to enable specialized functions such as ion movement triggered by membrane depolarization. The results support that VACC in OSN could be considered as a functional marker to study neurodevelopment

Melatonin Rescues the Dendrite Collapse Induced by the Pro-Oxidant Toxin Okadaic Acid in Organotypic Cultures of Rat Hilar Hippocampus

The pro-oxidant compound okadaic acid (OKA) mimics alterations found in Alzheimer’s disease (AD) as oxidative stress and tau hyperphosphorylation, leading to neurodegeneration and cognitive decline. Although loss of dendrite complexity occurs in AD, the study of this post-synaptic domain in chemical-induced models remains unexplored. Moreover, there is a growing expectation for therapeutic adjuvants to counteract these brain dysfunctions. Melatonin, a free-radical scavenger, inhibits tau hyperphosphorylation, modulates phosphatases, and strengthens dendritic arbors. Thus, we determined if OKA alters the dendritic arbors of hilar hippocampal neurons and whether melatonin prevents, counteracts, or reverses these damages. Rat organotypic cultures were incubated with vehicle, OKA, melatonin, and combined treatments with melatonin either before, simultaneously, or after OKA. DNA breaks were assessed by TUNEL assay and nuclei were counterstained with DAPI. Additionally, MAP2 was immunostained to assess the dendritic arbor properties by the Sholl method. In hippocampal hilus, OKA increased DNA fragmentation and reduced the number of MAP2(+) cells, whereas melatonin protected against oxidation and apoptosis. Additionally, OKA decreased the dendritic arbor complexity and melatonin not only counteracted, but also prevented and reversed the dendritic arbor retraction, highlighting its role in post-synaptic domain integrity preservation against neurodegenerative events in hippocampal neurons

Purinergic Signaling Pathway in Human Olfactory Neuronal Precursor Cells

Extracellular ATP and trophic factors released by exocytosis modulate in vivo proliferation, migration, and differentiation in multipotent stem cells (MpSC); however, the purinoceptors mediating this signaling remain uncharacterized in stem cells derived from the human olfactory epithelium (hOE). Our aim was to determine the purinergic pathway in isolated human olfactory neuronal precursor cells (hONPC) that exhibit MpSC features. Cloning by limiting dilution from a hOE heterogeneous primary culture was performed to obtain a culture predominantly constituted by hONPC. Effectiveness of cloning to isolate MpSC-like precursors was corroborated through immunodetection of specific protein markers and by functional criteria such as self-renewal, proliferation capability, and excitability of differentiated progeny. P2 receptor expression in hONPC was determined by Western blot, and the role of these purinoceptors in the ATP-induced exocytosis and changes in cytosolic Ca2+ ([Ca2+]i) were evaluated using the fluorescent indicators FM1-43 and Fura-2 AM, respectively. The clonal culture was enriched with SOX2 and OCT3/4 transcription factors; additionally, the proportion of nestin-immunopositive cells, the proliferation capability, and functionality of differentiated progeny remained unaltered through the long-term clonal culture. hONPC expressed P2X receptor subtypes 1, 3-5, and 7, as well as P2Y2, 4, 6, and 11; ATP induced both exocytosis and a transient [Ca2+]i increase predominantly by activation of metabotropic P2Y receptors. Results demonstrated for the first time that ex vivo-expressed functional P2 receptors in MpSC-like hONPC regulate exocytosis and Ca2+ signaling. This purinergic-triggered release of biochemical messengers to the extracellular milieu might be involved in the paracrine signaling among hOE cells