Divergent cerebrospinal fluid cytokine network induced by non-viral and different viral infections on the central nervous system.

Submitted by Nuzia Santos ([email protected]) on 2016-04-06T12:50:09Z No. of bitstreams: 1 Divergent cerebrospinal fluid cytokine .pdf: 6666541 bytes, checksum: b39732a64c755314d74c20e896a13597 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2016-04-06T13:24:06Z (GMT) No. of bitstreams: 1 Divergent cerebrospinal fluid cytokine .pdf: 6666541 bytes, checksum: b39732a64c755314d74c20e896a13597 (MD5)Made available in DSpace on 2016-04-06T13:24:06Z (GMT). No. of bitstreams: 1 Divergent cerebrospinal fluid cytokine .pdf: 6666541 bytes, checksum: b39732a64c755314d74c20e896a13597 (MD5) Previous issue date: 2015Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoraramento. Belo Horizonte, MG, Brasil/Instituto Hermes Pardini. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação de MedicinaTropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Patologia Anatomica. Belo Horizonte, MG, Brasil.Fundação de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil.Fundação de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil/Universidade Estadual do Amazonas. Manaus, AM, Brasil.BACKGROUND: Meningoencephalitis is one of the most common disorders of the central nervous system (CNS) worldwide. Viral meningoencephalitis differs from bacterial meningitis in several aspects. In some developing countries, bacterial meningitis has appropriate clinical management and chemotherapy is available. Virus-associated and virus not detected meningoencephalitis are treatable, however, they may cause death in a few cases. The knowledge of how mediators of inflammation can induce disease would contribute for the design of affordable therapeutic strategies, as well as to the diagnosis of virus not detected and viral meningoencephalitis. Cytokine-induced inflammation to CNS requires several factors that are not fully understood yet. METHODS: Considering this, several cytokines were measured in the cerebrospinal fluid (CSF) of patients with undiagnosed and viral meningoencephalitis, and these were correlated with cellularity in the CSF. RESULTS: The results demonstrate that an altered biochemical profile alongside increased cellularity in the cerebrospinal fluid is a feature of patients with meningoencephalitis that are not associated with the detection of virus in the CNS (P < 0.05). Moreover, HIV-positive patients (n = 10) that evolve with meningoencephalitis display a distinct biochemical/cytological profile (P < 0.05) in the cerebrospinal fluid. Meningoencephalitis brings about a prominent intrathecal cytokine storm regardless of the detection of virus as presumable etiological agent. In the case of Enterovirus infection (n = 13), meningoencephalitis elicits robust intrathecal pro-inflammatory cytokine pattern and elevated cellularity when compared to herpesvirus (n = 15) and Arbovirus (n = 5) viral infections (P < 0.05). CONCLUSION: Differences in the cytokine profile of the CSF may be unique if distinct, viral or presumably non-viral pathways initially trigger the inflammatory response in the CNS