Invitro Regeneration And GUS Expression Studies In Ground Nut (Arachis Hypogaea L.) Variety ICGV 15311.

Ground nut is an important source of food,edible oil,dietary vitamin, minerals, proteins, animal feed etc. In this research, an efficient of regeneration and Agrobacterium mediated genetic transformation with GUS expression is reported in a commercially important variety ICGV 15311, using embryo axis, Leaf, Half cut embryo axis, Cotyledonary node. Various combinations and concentrations of plant growth regulators are used to obtain multiple shooting and rooting.MS (Murashig and Skoog) medium supplemented with various concentrations of Auxins (IBA,NAA,IAA and 2,4D) and Cytokinins (BAP and KIN).Explants cultured on 2,4 D and KIN expressed only swelling and enlargement without further development.BAP and NAA showed more shoots on embryo axis explants on medium concentration and combination of BAP (2.0 mg/l) and NAA(0.5mg/l).Rooting obtained on IBA (2.0mg/l). The bacterial culture 1.0 OD 600, incubation with 50 µl acetosyringone and co cultivation period of 3 days were found to be efficient for transformation. Tansformed putatives grown on shooting and rooting medium and remained healthy putatives transferred to soil for hardening