Schematic presentation of sampling lesions with tape strip discs, DNA isolation and further identification of <i>Leishmania</i> species.

<p>1) patient´s lesion 2) disc 3) disc applied to patient´s lesion 4) even pressure applied on lesion using a plunger 5) sterile removal of disc from the lesion 6) DNA isolation from disc 7) confirmation of <i>Leishmania</i> by kDNA1 8) species-specific identification (PCR ITS-RFLP).</p

Diagnostic accuracy of parasite culture from biopsies and tape strip disc.

<p>Diagnostic accuracy of parasite culture from biopsies and tape strip disc.</p

Sensitive areas for sampling.

<p>A) eyelid B) around the eye C) ear D) on the lips.</p

Disease duration, gender distribution and infecting species of <i>Leishmania</i> in CL patients with acute, chronic or healed disease.

<p>Disease duration, gender distribution and infecting species of <i>Leishmania</i> in CL patients with acute, chronic or healed disease.</p

Clinical profiles of 119 cutaneous leishmaniasis patients.

<p>Clinical profiles of 119 cutaneous leishmaniasis patients.</p

Agarose gel electrophoresis of amplified ITS1 regions of standard reference strains.

<p>H₂O (lane 1) <i>L</i>. <i>major</i> (lane 2) and <i>L</i>. <i>tropica</i> (lane 3) and from CL patients (lanes 4–9) in panel A and restriction endonuclease <i>Hae</i>III digestion is shown in panel B. In both panels, MW representative of 100-bp ladder as molecular size marker. Patients in samples 2, 3, 4, 6, 7 and 9 are infected with <i>L</i>. <i>tropica</i> and patients in samples 1, 5 and 8 are infected with <i>L</i>. <i>major</i>.</p

Site characteristics in Old World settings.

<p>Site characteristics in Old World settings.</p

Agreement on key parameters by OWCL and NWCL clinical researchers.

<p>‘Standardized’ criteria are those as proposed in the reference paper Olliaro et al, 2013⁹; ‘updated’ criteria are those resulting from the consultation.</p

Site characteristics in New World settings.

<p>Site characteristics in New World settings.</p