10 research outputs found
Chromosome banding, DNA methylation patterns and chromatin organisation in Zingeria biebersteniana (Claus) P. Smirnov.
rDNA sequences, chromatin organisatin, chromosome banding and automated karyotype analysis in Vicia michauxii Sprengel.
Cytology of Vicia species. Vicia michauxii Sprengel.: automated caryotype analysis, chromatin organisation, rDNA sequences and chromosome banding.
Characterization of normal and “albino” phenotypes in Erythrina crista-galli
We present here a characterization of Erythrina crista-galli L. (syn: Erythrina lamifolia Jacq.) seedlings, obtained from a plant from the Botanical Garden of Pisa University. This plant produces seeds that, during germination, have shown two different seedling phenotypes: normal (NT, 75%) and "albino" types (AT, 25%). Albino seedlings survive only 8–9 weeks and their growth is dramatically reduced when compared with wild type seedlings. Biochemical investigations have shown that albino seedlings completely lack chlorophyll and carotenoids and also soluble sugar levels are lower than in the normal type. We have also conducted sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) experiments and silver staining analysis on different protein extracts from shoots and leaves of both phenotypes, and demonstrated strong differences in protein patterns. The almost total absence of putative small and large RuBisCo bands in albino seedlings should be emphasized. We have also microspectrophotometrica..