16 research outputs found
Physical characterisation of an alginate/lysozyme nano-laminate coating and its evaluation on ‘coalho’ cheese shelf life
This work aimed at the characterisation of a nanolaminate
coating produced by the layer-by-layer methodology
and its evaluation on the preservation of ‘Coalho’ cheese.
Initially, five alternate layers of alginate and lysozyme were
assembled in an aminolysed/charged polyethylene terephthalate
(A/C PET) and physically characterised by UV/VIS
spectroscopy, contact angle, water vapour (WVTR) and oxygen
(OTR) transmission rates and scanning electron microscopy.
Afterwards, the same methodology was used to
apply the nano-laminate coating in ‘Coalho’ cheese and its
shelf life was evaluated during 20 days in terms of mass
loss, pH, lipid peroxidation, titratable acidity and microbial
count. UV/VIS spectroscopy and contact angle analyses
confirmed the layers’ deposition and the successful assembly
of nano-laminate coating on A/C PET surface. The coating
presented WVTR and OTR values of 1.03×10−3 and 1.28×
10−4 g m−2 s−1, respectively. After 20 days, coated cheese
showed lower values of mass loss, pH, lipidic peroxidation,
microorganisms’ proliferation and higher titratable acidity in
comparison with uncoated cheese. These results suggest that
gas barrier and antibacterial properties of alginate/lysozyme
nanocoating can be used to extend the shelf life of ‘Coalho’
cheese.The author Bartolomeu G. de S. Medeiros is recipient of a scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES-Brazil). The author Marthyna P. Souza is recipient of a scholarship from Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE, Brazil) and was recipient of a scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES/PDEE-Brazil). The authors Ana C. Pinheiro, Ana I. Bourbon and Miguel A. Cerqueira are recipients of a fellowship (SFRH/BD/48120/2008, SFRH/BD/73178/2010 and SFRH/BPD/72753/2010, respectively), supported by Fundacao para a Ciencia e Tecnologia, POPH-QREN and FSE (FCT, Portugal). Maria G. Carneiro-da-Cunha express is gratitude to the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for research grant. The present work was supported by CAPES/PROCAD/NF/1415/2007. The support of EU Cost Action FA0904 is gratefully acknowledged
Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects
Aerogels prepared from aqueous dispersions of anionic and cationic cellulose nanofibrils (CNFs) were investigated as solid supports for enzymes and silver nanoparticles and to elicit a sustained antibacterial effect. The imparted stabilization in dry conditions was studied with aerogels that were cast after mixing the enzymes with CNFs followed by dehydration (freeze-drying). The activity of lysozyme immobilized in the given CNF system was analyzed upon storage in liquid and air media. In contrast with aqueous solutions of free, unbound enzyme, which lost activity after the first day, the enzyme immobilized physically in unmodified and cationic CNF presented better stability (activity for a longer time). However, the enzyme activity was reduced in the case of anionic CNF, which was prepared by TEMPO-mediated oxidation (TO-CNF). Both humidity and temperature reduced the stability of the enzyme immobilized in the respective CNF aerogel. The antibacterial activity of CNF aerogels carrying lysozyme was also tested against gram-negative and gram-positive bacteria. The results were compared with those obtained from CNF systems loaded with silver nanoparticles (AgNP) after in situ synthesis via UV reduction. Storage in cold or dry conditions preserved the activity and antibacterial performance of enzyme-loaded CNF aerogels. As expected, the lysozyme-containing aerogels showed lower inhibition than the AgNP-containing aerogel. In this latter case, the antibacterial activity depended on the concentration and size of the nanoparticles. Compared to unmodified CNF and TO-CNF, the aerogels prepared with cationic CNF, loaded with either lysozyme or AgNPs, showed remarkably better antibacterial activity. Similar experiments were conducted with horseradish peroxidase, which confirmed, to different degrees, the observations derived from the lysozyme systems. Overall, the results indicate that non-toxic and biodegradable CNF is a suitable support for bio-active materials and is effective in protecting and retaining enzymatic and antibacterial activities