Diagnostic approach and epidemiology of Microbial Keratitis: findings from an Italian Tertiary Care center

Rapid identification of causative microorganisms of microbial keratitis (MK) and knowledge of the most common local pathogens are prerequisites for rational antimicrobial therapy. We retrospectively reviewed the characteristics of MK diagnosed at the IRCCS Arcispedale Santa Maria Nuova of Reggio Emilia (Italy) in a 5-years period, where the Ophthalmologist Unit is a reference center for corneal infections. During the study period, 183 MK were evaluated through corneal scrapings cultures. The positivity rate was 54,1%. A total of 107 microorganisms have been isolated: Acanthamoeba species was the etiologic agent in 19 cases. Pseudomonas aeruginosa and Staphylococcus aureus were more frequently isolated in bacterial keratitis, while Fusarium spp., Candida albicans, and Alternaria alternata were predominant among the fungal isolates. Strict cooperation between ophthalmologists and clinical microbiologists is advisable to allow the best diagnostic approach for MK

Evolution of Ciona intestinalis Tumor necrosis factor alpha (CiTNFα): Polymorphism, tissues expression, and 3D modeling

Although the Tumor necrosis factor gene superfamily seems to be very conserved in vertebrates, phylogeny, tissue expression, genomic and gene organization, protein domains and polymorphism analyses showed that a strong change has happened mostly in invertebrates in which protochordates were a constraint during the immune-molecules history and evolution. RT PCR was used to investigate differential gene expression in different tissues. The expression shown was greater in the pharynx. Single-nucleotide polymorphism has been investigated in Ciona intestinalis Tumor necrosis factor alpha (CiTNFα) mRNA isolated from the pharynx of 30 ascidians collected from Licata, Sicily (Italy), by denaturing gradient gel electrophoresis (DGGE). For this analysis, CiTNFα nucleotide sequence was separated into two fragments, TNF-1 and -2, respectively, of 630 and 540 bp. We defined 23 individual DGGE patterns (named 1 to 10 for TNF-1 and 1 to 13 for TNF-2). Five patterns for TNF-1 accounted for <10% of the individuals, whereas the pattern 13 of TNF-2 accounted for >20% of the individuals. All the patterns were verified by direct sequencing. Single base-pair mutations were observed mainly within COOH-terminus, leading to 30 nucleotide sequence variants and 30 different coding sequences segregating in two main different clusters. Although most of the base mutations were silent, four propeptide variants were detected and six amino acid replacements occurred within COOH-terminus. Statistical tests for neutrality indicated negative selection pressure on signal and mature peptide domains, but possible positive selection pressure on COOH-terminus domain. Lastly we displayed the in silico 3D structure analysis including the CiTNFα variable region

A Tight Interaction between the Native Seagrass Cymodocea nodosa and the Exotic Halophila stipulacea in the Aegean Sea Highlights Seagrass Holobiont Variations

Seagrasses harbour bacterial communities with which they constitute a functional unit called holobiont that responds as a whole to environmental changes. Epiphytic bacterial communities rapidly respond to both biotic and abiotic factors, potentially contributing to the host fitness. The Lessepsian migrant Halophila stipulacea has a high phenotypical plasticity and harbours a highly diverse epiphytic bacterial community, which could support its invasiveness in the Mediterranean Sea. The current study aimed to evaluate the Halophila/Cymodocea competition in the Aegean Sea by analysing each of the two seagrasses in a meadow zone where these intermingled, as well as in their monospecific zones, at two depths. Differences in holobionts were evaluated using seagrass descriptors (morphometric, biochemical, elemental, and isotopic composition) to assess host changes, and 16S rRNA gene to identify bacterial community structure and composition. An Indicator Species Index was used to identify bacteria significantly associated with each host. In mixed meadows, native C. nodosa was shown to be affected by the presence of exotic H. stipulacea, in terms of both plant descriptors and bacterial communities, while H. stipulacea responded only to environmental factors rather than C. nodosa proximity. This study provided evidence of the competitive advantage of H. stipulacea on C. nodosa in the Aegean Sea and suggests the possible use of associated bacterial communities as an ecological seagrass descriptor

Aquaculture of emerging species in North-Eastern Atlantic and Mediterranean Sea: a systematic review on sea cucumber farming and potential development

Sea cucumbers are considered a traditional food delicacy in Asian countries. In last decades high market demand, uncontrolled exploitation and inadequate management have led to stock overexploitation, driving commercial interest in new target species and new areas. In recent years, Mediterranean and North-East Atlantic sea cucumbers have become fishing targets, and, due to overfishing, a significant decline of wild populations have been observed, with detrimental effects on benthic communities and ecosystems. Indeed, in addition to being an important economic resource, sea cucumbers play a key ecological role in benthic dynamics where they are involved as ecosystem engineers in the processing of organic matter in the detrital food web pathway. In this context, aquaculture can play a crucial role in supporting the conservation of natural stocks by reducing harvesting pressure on wild populations and enabling restoration programmes for depleted stocks. Commercial sea cucumber farming is already an established reality in the Indo-Pacific region, where it has become one of the most profitable sectors of aquaculture production. In Europe, on the other hand, sea cucumber aquaculture is still an emerging sector, whose development is hampered by poor and fragmented information on the farming requirements of native species. In this context, this systematic review was carried out with the aim of providing a comprehensive summary of the state of the art of farming practices of the European sea cucumbers considered as a target of commercial fisheries. A total of 34 original articles were included in this review, after title, abstract and full-text screening. The results highlighted a sharp increase in the number of studies over the last six years. Portugal, Turkey and Italy are the countries where most research has been carried out. Among the selected species, Holothuria tubulosa was the most studied, while only one article was found on Parastichopus regalis. The main aims of this review were i) to gather all available literature on the farming practices of North-East Atlantic and Mediterranean sea cucumber species, ii) to outline the most common farming practices, iii) to identify gaps and future directions in this emerging aquaculture sector