Talaromyces atroroseus, a new species efficiently producing industrially relevant red pigments

Some species of Talaromyces secrete large amounts of red pigments. Literature has linked this character to species such as Talaromyces purpurogenus, T. albobiverticillius, T. marneffei, and T. minioluteus often under earlier Penicillium names. Isolates identified as T. purpurogenus have been reported to be interesting industrially and they can produce extracellular enzymes and red pigments, but they can also produce mycotoxins such as rubratoxin A and B and luteoskyrin. Production of mycotoxins limits the use of isolates of a particular species in biotechnology. Talaromyces atroroseus sp. nov., described in this study, produces the azaphilone biosynthetic families mitorubrins and Monascus pigments without any production of mycotoxins. Within the red pigment producing clade, T. atroroseus resolved in a distinct clade separate from all the other species in multigene phylogenies (ITS, β-tubulin and RPB1), which confirm its unique nature. Talaromyces atroroseus resembles T. purpurogenus and T. albobiverticillius in producing red diffusible pigments, but differs from the latter two species by the production of glauconic acid, purpuride and ZG-1494α and by the dull to dark green, thick walled ellipsoidal conidia produced. The type strain of Talaromyces atroroseus is CBS 133442

Data - Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 2. Nonionic micellar systems

<p><strong>Overview</strong></p> <p>The production of recombinant biopharmaceuticals is highly dependent of a proper choice of the downstream processing stages. Particularly, the purification that must ensure that all the endotoxins (lipopolysaccharide - LPS) are efficiently removed from the final product. This dataset contains the raw data and statistical analysis for the research entitled - "Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 2. Nonionic micellar systems". </p> <p><strong>Info</strong></p> <p>ANOVA_Turkey_Sub.R <- code for ANOVA analysis in R statistic 3.3.3    <br> glm.R <- code for GLM analysis in R statistic 3.3.3<br> K&REC_ORG_ANOVA.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) and recover (REC) for ANOVA analysis</p> <p>K_ORG_ANOVA.docx <-  File with ANOVA result of partition coefficient (K) for GFP</p> <p>REC_ORG_ANOVA.docx  <- File with ANOVA result of partition coefficient (REC) for GFP</p> <p>REM_LPS_ORG_ANOVA.csv  <- File with raw values organized in a spreadsheet of LPS removal for ANOVA analysis</p> <p>REM_LPS_ORG_ANOVA.docx  <- File with ANOVA result of removal of LPS</p> <p>Stability__ORG_ANOVA.csv <- File with raw values organized in a spreadsheet of GFP stability for ANOVA analysis</p> <p>Stability__ORG_ANOVA.docx  <- File with ANOVA result of GFP stability</p> <p>K_ORG_glm_005.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.05M salt assays    </p> <p>K_ORG_glm_005.doc <- File with GLM analysis of GFP partition coefficient (K) in 0.05M salt assays  </p> <p>K_ORG_glm_005_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.05M salt assays  </p> <p>K_ORG_glm_025.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays    </p> <p>K_ORG_glm_025.doc <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays  </p> <p>K_ORG_glm_025_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.25M salt assays  </p> <p>REC_ORG_glm_005.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.05M salt assays</p> <p>REC_ORG_glm_005.doc <-  File with GLM analysis of GFP recover (REC) in 0.05M salt assays    </p> <p>REC_ORG_glm_005_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.05M salt assays   </p> <p>REC_ORG_glm_025.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.25M salt assays</p> <p>REC_ORG_glm_025.doc <-  File with GLM analysis of GFP recover (REC) in 0.25M salt assays    </p> <p>REC_ORG_glm_025_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays   </p> <p>REM_ORG_glm_005.csv <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.05M salt assays</p> <p>REM_ORG_glm_005.doc <-  File with GLM analysis of LPS removal (REM) in 0.05M salt assays  </p> <p>REM_ORG_glm_005_QQ.png <-  Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays</p> <p>REM_ORG_glm_025.csv <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.25M salt assays</p> <p>REM_ORG_glm_025.doc <-  File with GLM analysis of LPS removal (REM) in 0.25M salt assays </p> <p>REM_ORG_glm_025_QQ.png <-  Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays</p> <p> </p> <p><strong>Annotation</strong></p> <p>12/12 - Concentration of 12% of each polymer PEG/NaPA</p> <p>16/16 - Concentration of 16% of each polymer PEG/NaPA</p> <p>P/N -  PEG/NaPA</p> <p>10e4, 10e5, 10e6 - Concentration of LPS in scientific notation - 10000, 100000, 100000 EU/mL</p> <p>poly - Polymer</p> <p>salt - Salt concentration in the assay</p> <p>tsalt - Type of salt in the assay (NaCl, KNO3, KI and Li2SO4)</p> <p>lps - lipopolysaccharide</p> <p>K - GFP partition coefficient</p> <p>REM - LPS removal</p> <p>REC - GFP recover</p> <p>wo_salt - Assay without salt addition</p> <p><strong>Acknowledgements</strong></p> <p>The authors are grateful for financial support from FAPESP (São Paulo Research Foundation, Brazil) through the following projects: 2005/60159-7; 2007/51978-0; 2014/16424-7; and 2014/19793-3. The authors also acknowledge the support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) through the process #0366/09-9 and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).</p> <p><strong>Consider citing our work. </strong></p> <p>1. Work in progress...</p

Data - Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems

<p><strong>Overview</strong></p> <p>The production of recombinant biopharmaceuticals is highly dependent of a proper choice of the downstream processing stages. Particularly, the purification that must ensure that all the endotoxins (lipopolysaccharide - LPS) are efficiently removed from the final product. This dataset contains the raw data and statistical analysis for the research entitled - "Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems". </p> <p><strong>Info</strong></p> <p>ANOVA_Turkey_Sub.R <- code for ANOVA analysis in R statistic 3.3.3    <br> glm.R <- code for GLM analysis in R statistic 3.3.3<br> K&REC_LPS_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) and recover (REC) for ANOVA analysis<br> K&REC_LPS_PEG_NaPA_K.docx <- File with ANOVA result of partition coefficient (K) for GFP<br> K&REC_LPS_PEG_NaPA_REC.docx <- File with ANOVA result of recover (REC) for GFP         <br> K_GFP_Pol_005.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.05M salt assays    <br> K_GFP_Pol_005.doc <- File with GLM analysis of GFP partition coefficient (K) in 0.05M salt assays  <br> K_GFP_Pol_005_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.05M salt assays  <br> K_GFP_Pol_025.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays  <br> K_GFP_Pol_025.doc  <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays  <br> K_GFP_Pol_025_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.25M salt assays         <br> REC_GFP_Pol_005.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.05M salt assays         <br> REC_GFP_Pol_005.doc  <- File with GLM analysis of GFP recover (REC) in 0.05M salt assays    <br> REC_GFP_Pol_005_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.05M salt assays   <br> REC_GFP_Pol_025.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.25M salt assays          <br> REC_GFP_Pol_025.doc  <- File with GLM analysis of GFP recover (REC) in 0.25M salt assays <br> REC_GFP_Pol_025_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays        <br> REM_LPS_PEG_NaPA.docx  <- File with ANOVA result of LPS removal          <br> REM_LPS_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of LPS removal for ANOVA analysis<br> Stability_GFP_PEG_NaPA.docx <- File with ANOVA result of GFP stability<br> Stability_GFP_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of GFP stability results for ANOVA analysis</p> <p>REM_LPS_Pol_005.csv  <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.05M salt assays         <br> REM_LPS_Pol_005.doc  <- File with GLM analysis of LPS removal (REM) in 0.05M salt assays    <br> REM_LPS_Pol_005_QQ.png <- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.05M salt assays  <br> REM_LPS_Pol_025.csv  <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.25M salt assays         <br> REM_LPS_Pol_025.doc   <- File with GLM analysis of LPS removal (REM) in 0.25M salt assays    <br> REM_LPS_Pol_025_QQ.png <- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays</p> <p>K_GFP_Pol_025_NaCl_Li2SO4.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays comparing NaCl and Li2SO4 effect <br> K_GFP_Pol_025_NaCl_Li2SO4.doc  <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays comparing NaCl and Li2SO4 effect <br> K_GFP_Pol_025_NaCl_Li2SO4_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays comparing NaCl and Li2SO4 effect   </p> <p>REM_LPS_Pol_KI_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KI assays comparing salt concentration effect  <br> REM_LPS_Pol_KI_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in KI assays comparing salt concentration effect<br> REM_LPS_Pol_KI_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in KI assays comparing salt concentration effect<br> REM_LPS_Pol_KNO3_0.05_vs_0.25.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KNO3 assays comparing salt concentration effect  <br> REM_LPS_Pol_KNO3_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in KNO3 assays comparing salt concentration effect<br> REM_LPS_Pol_KNO3_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in KNO3 assays comparing salt concentration effect<br> REM_LPS_Pol_Li2SO4_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in Li2SO4 assays comparing salt concentration effect  <br> REM_LPS_Pol_Li2SO4_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in Li2SO4 assays comparing salt concentration effect<br> REM_LPS_Pol_Li2SO4_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in Li2SO4 assays comparing salt concentration effect<br> REM_LPS_Pol_NaCl_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in NaCl assays comparing salt concentration effect  <br> REM_LPS_Pol_NaCl_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in NaCl assays comparing salt concentration effect <br> REM_LPS_Pol_NaCl_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in NaCl assays comparing salt concentration effect</p> <p> </p> <p><strong>Annotation</strong></p> <p>12/12 - Concentration of 12% of each polymer PEG/NaPA</p> <p>16/16 - Concentration of 16% of each polymer PEG/NaPA</p> <p>P/N -  PEG/NaPA</p> <p>10e4, 10e5, 10e6 - Concentration of LPS in scientific notation - 10000, 100000, 100000 EU/mL</p> <p>poly - Polymer</p> <p>salt - Salt concentration in the assay</p> <p>tsalt - Type of salt in the assay (NaCl, KNO3, KI and Li2SO4)</p> <p>lps - lipopolysaccharide</p> <p>K - GFP partition coefficient</p> <p>REM - LPS removal</p> <p>REC - GFP recover</p> <p>wo_salt - Assay without salt addition</p> <p><strong>Acknowledgements</strong></p> <p>The authors are grateful for financial support from FAPESP (São Paulo Research Foundation, Brazil) through the following projects: 2005/60159-7; 2007/51978-0; 2014/16424-7; and 2014/19793-3. The authors also acknowledge the support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) through the process #0366/09-9 and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).</p> <p><strong>Consider citing our work. </strong></p> <p>1. Work in progress...</p