Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats

Cyanobacteria are among the most diverse morphological microorganisms that inhabit a great variety of habitats. Their presence in the Azores, a volcanic archipelago of nine islands in the middle of the North Atlantic Ocean, has already been reported. However, due to the high diversity of cyanobacteria habitats, their biodiversity is still understudied, mainly in extreme environments. To address this, a total of 156 cyanobacteria strains from Azores lakes, streams, thermal and terrestrial habitats were isolated. Identification was made based on a polyphasic approach using classical taxonomy (morphological characteristics and environmental data) and phylogeny among 81 strains assessed by maximum likelihood and Bayesian analysis of 16S rDNA partial sequences. The 156 isolates showed a high genera diversity (38) belonging to the orders Chroococcales, Nostocales, Oscillatoriales, and Synechococcales. Eleven new genera for the Azores habitats are here reported, reinforcing that cyanobacteria biodiversity in these islands is still much understudied. Phylogenetic analysis showed 14 clusters associated with these cyanobacteria orders, with evidence for six new genera and valuable information towards Microchaete/Coleospermum taxonomic revision that better reflects species environmental distribution. These results emphasize the need for cyanobacteria taxonomy revisions, through polyphasic studies, mainly in Synechococcales order and in the Microchaete/Coleospermum, Nostoc, and Anabaena genera.Rita Cordeiro was supported by a Ph.D. grant (M3.1.a/F/017/2015) from the Fundo Regional da Ciência e Tecnologia (FRCT). This work was funded by FEDER funds through the Interreg-MAC 2014-2020 Program under the projects REBECA - Red de excelencia en biotecnología azul (algas) de la región macaronesia (MAC1.1a/060) and REBECA-CCT - Red de Excelencia en Biotecnología Azul de la Región Macaronésica. Consolidación, Certificación y Transferencia (MAC2/1.1b/269), and by Portuguese National Funds, through FCT - Fundação para a Ciência e a Tecnologia, the European Union, QREN, FEDER, COMPETE, by funding the CIBIO/InBIO (project UID/BIA/ 50027/2013 and POCI-01-0145-FEDER-006821)

A comparison of mass parameters determination using capacitive and optical sensors

AbstractThis paper presents a comparison study between the use of 1mm resolution capacitive and optical sensors for the determination of yarn mass parameters. A parallel plate capacitive sensor to determine yarn mass variations and a yarn diameter and hairiness determination solution using optical sensors and integrating optical signal processing based on Fourier analysis are described. As there is a high correlation between yarn diameter and yarn mass, it is possible to determine yarn mass and infer variations in yarn diameter and vice-versa. Moreover, by optically detecting the degree of yarn hairiness, one can quantify its influence on the capacitive sensor mass variation measurements. Here we present the results of a signal processing analysis and statistical description of measurements carried out on a 100% cotton 295 g/km linear mass yarn. We conclude that an accurate yarn characterization can be carried out using optical sensors alone, reducing systems cost, complexity and increasing efficiency

Oral Contraceptive Use and Clinical Outcomes in Patients with Multiple Sclerosis

Experimental and clinical data suggest a role of sex steroids in the pathogenesis of multiple sclerosis (MS). Scant information is available about the potential effect of oral contraceptive (OC) use on the prognosis of the disease. We aimed to evaluate this. The study population consisted of 132 women with relapsing-remitting MS before receiving disease modifying treatment and a mean disease duration 6.2 (SD 5.1) years. Three groups of patients were distinguished according to their OC behavior: [1] never-users, patients who never used OC [2] past-users, patients who stopped OC use before disease onset, and [3] after-users, those who used these drugs after disease onset. Multiple linear and logistic regression models were used to analyze the association between oral contraceptive use and annualized relapse rates, disability accumulation and severity of the disease. After-user patients had lower Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS) values than never users (p<0.001 and p=0.002, respectively) and past users (p=0.010 and p=0.002, respectively). These patients were also more likely to have a benign disease course (MSSS<2.5) than never and past users together (OR: 4.52, 95%CI: 2.13-9.56, p<0.001). This effect remained significant after adjustment for confounders, including smoking and childbirths (OR: 2.97, 95%CI: 1.24, 6.54, p=0.011 and for MSSS β: -1.04; 95% C.I. -1.78, -0.30, p=0.006). These results suggest that OC use in women with relapsing-remitting MS is possible associated with a milder disabling disease course

Indirect rapid tooling with aluminium and milled fibre reinforced resins

It is well known that Rapid Prototyping (RP) and Rapid Tooling (RT) are fundamental technologies to reduce the time tomarket and consequently increase productivity and competitiveness of companies working in different industrial sectors.RT with epoxy resins is presently accepted as an established process of indirect rapid tooling. However, patents,processing difficulties and companies limitations disable a wider implementation of this technology.This paper presents the experimental results of a detailed work, conducted at INEGI, to manufacture moulds for plasticinjection, with tailored properties, using RP models and aluminium filled and fibres reinforced epoxy resins

The effect of aluminium granulometry on the behaviour of filled epoxy resins for rapid tooling

Aluminium filled epoxies moulds have beenused in indirect rapid tooling. These moulds, whichhave a low cost processing, are very competitive,when applied in the manufacturing of low volumeseries.The geometry and the size distribution of thealuminium particles affect significantly the powderpacking density, the resin ratio, the thermalconductivity, the curing time, the homogeneity andthe mechanical characteristics of the tool.Using three high temperature epoxy resins itwas possible to understand the resin behaviour inthe presence of the aluminium filler and formulatefilled resins with the best compromise ofmechanical, thermal and processing performance

Viabilidade econômica do acabamento de cordeiros em pastagens cultivadas de capim gramão e tanzânia.

O presente trabalho, realizado na Embrapa Caprinos, em Sobral-CE, avaliou a terminação de cordeiros em pastagens de capim gramão (Cynodon dactylon (L.)) e capim tanzânia (panicum maximum cv. tanzania), sob duas taxas de lotação. O período de terminação foi de 83 dias. O lucro obtido com a terminação de cordeiros em pastagem de gramão e venda dos animais em pé foi de R$1,58/cordeiro e R$ 3,77/cordeiro, para taxas de lotação de 40 e 60 animais/ha, respectivamente. Nas áreas de tanzânia, o lucro obtido foi de R$4,31/cordeiro e R$ 2,64/cordeiro, para taxas de lotação de .40 e 60 animais/ha, respectivamente. Entretanto, cada ha permitiu a terminação de 4,4 lotes por ano. Com isso, o lucro anual da terminação no gramão foi de R$277,30/ha e R$ 995,66/ha nas taxas de lotação de 40 e 60 animais/ha, respectivamente. A terminação à pasto no tanzânia proporcionou um lucro anual de R$758,64/ha e R$ 697,65/ha nas taxas de lotação de 40 e 60 animais/ha, respectivamente. A venda dos animais abatidos proporcionou um lucro anual de R$94, 79/ha e de R$ I 233, 23/ha para taxas de lotação de 40 e 60 animais/ha, respectivamente, no gramão. Já em pastagem . de tanzânia, a venda dos animais abatidos proporcionou um lucro anual de R$198,79/ha e R$ 891,95/ha para as taxas de lotação de 40 e 60 animais/ha, respectivamente. A terminação de cordeiros em pastagem de capim gramão e de tanzânia foi economicamente viável.CD ROM

Exploitation of filamentous and picoplanktonic cyanobacteria for cosmetic applications: potential to improve skin structure and preserve dermal matrix components

The use of natural products in skin care formulations gained interest as a concern for modern societies. The undesirable side effects of synthetic compounds, as well as the associated environmental hazards, have driven investigation on photosynthetic organisms as sustainable sources of effective and environmentally friendly ingredients. The use of natural extracts in cosmetics has been highlighted and, along with plants and algae, cyanobacteria have come into focus. Due to their low culture demands, high grow rates and ability to produce a wide variability of bioactive metabolites, cyanobacteria emerged as an economic and sustainable base for the cosmetic industry. In this study, we evaluated the potential of ethanol extracts of picocyanobacteria strains of the genera Cyanobium and Synechocystis and filamentous strains of the genera Nodosilinea, Phormidium and Tychonema for skin applications, with focus in the field of anti-aging. The extracts were analyzed for their pigment profile, phenolic content, antioxidant potential, cytotoxicity against keratinocytes (HaCat), fibroblasts (3T3L1), endothelial cells (hCMEC/D3) and capacity to inhibit hyaluronidase (HAase). The total carotenoid content ranged from 118.69 to 383.89 μg g−1 of dry biomass, and the total phenolic content from 1.07 to 2.45 mg GAE g−1. Identified carotenoids consisted of zeaxanthin, lutein, canthaxanthin, echinenone and β-carotene, with zeaxanthin and lutein being the most representative (49.82 and 79.08 μg g−1, respectively). The highest antioxidant potential was found for Phormidium sp. LEGE 05292 and Tychonema sp. LEGE 07196 for superoxide anion radical (O2•−) scavenging (IC50 of 822.70 and 924 μg mL−1, respectively). Low or no cytotoxicity were registered. Regarding HAase inhibition, Tychonema sp. LEGE 07196 and Cyanobium sp. LEGE 07175 showed the best IC50 (182.74 and 208.36 μg mL−1, respectively). In addition, an increase in fibroblast proliferation was registered with these same strains. From this work, the ethanol extracts of the species Tychonema sp. and Cyanobium sp. are particularly interesting for their potential application in anti-aging formulations, once they stimulated fibroblast proliferation and inhibit hyaluronic acid digestion.This work was done in the framework of the projects: BLUEHUMAN-BLUE biotechnology as a road for innovation on HUMAN’s health aiming smart growth in Atlantic Area-EAPA_151/2016 of the Interreg Atlantic Area Programme funded by the European Regional Development Fund; EnhanceMicroAlgae - High added-value industrial opportunities for microalgae in the Atlantic Area (EAPA_338/2016) of the Interreg Atlantic Area Programme funded by the European Regional Development Fund; ALGAVALOR - MicroALGAs: integrated production and valorization of biomass and its various applications - SI I&DT no. 352234-supported by the PORTUGAL 2020 through the European Regional Development Fund; and supported by the FCT Projects UIDB/04423/2020 and UIDP/04423/2020. The authors acknowledge the support and the use of resources of EMBRC-ERIC, specifically of the Portuguese infrastructure node of the European Marine Biological Resource Centre (EMBRC-PT) CIIMAR–PINFRA/22121/2016–ALG-01-0145-FEDER-022121, financed by the European Regional Development Fund (ERDF) through COMPETE2020-Operational Programme for Competitiveness and Internationalisation (POCI) and national funds through FCT/MCTES