Serious damage by Diplodia africana on Pinus pinea in the Vesuvius National Park.

In some municipalities, located within the boundaries of the Vesuvius National Park, several area with forest cover of Pinus pinea showed severe withering of the crowns and damage to pine cones. In the present study, we have isolated in the period may 2013 ??? may 2014 from Ercolano, San Sebastiano, Terzigno, Torre del Greco and Trecase an anamorphic form of Botryosphaeriaceae. The latter cause dieback and serious canker on several woody plants, including species of Pinus. Morphological and cultural characteristics as well as DNA sequence data (5.8S rDNA, ITS-1 and ITS-4) were made on 30 isolates obtained from 5 municipalities. All strains belonged to only two species: Botryosphaeria dothidea and its anamorph, Diplodia africana. These two fungi were present on all pine cones collected and analyzed. Finally we carried out growth assays at different temperatures: 8 °C, 18 °C and 28 °C. All fungi found the optimum of growth at 28°C while at 8°C we noted the lowest growth. This seems to be the first report of D. africana on Pinus species in Campania Region

Multiple roles and effects of a novel Trichoderma hydrophobin

Fungi belonging to the genus Trichoderma are among the most active and ecologically successful microbes found in natural environments, as they are able to use a variety of substrates and affect the growth of other microbes and virtually any plant species. We isolated and characterized a novel type II hydrophobin secreted by the biocontrol strain MK1 of Trichoderma longibrachiatum. The corresponding gene (Hytlo1) has a multiple role in the Trichoderma-plant-pathogen three-way interaction, while the purified protein displayed a direct antifungal as well as a MAMP and a plant growth promotion (PGP) activity. Leaf infiltration with the hydrophobin systemically increased resistance to pathogens and activated defence-related responses involving ROS, SOD, oxylipins, phytoalexins and PR-proteins formation or activity. The hydrophobin was found to enhance development of a variety of plants when applied at very low doses. It particularly stimulated root formation and growth, as demonstrated also by transient expression of the encoding gene in tobacco and tomato. Targeted knock-out of Hytlo1 significantly reduced both antagonistic and PGP effect of the WT strain. We conclude that this protein represents a clear example of a molecular factor developed by Trichoderma to establish a mutually beneficial interaction with the colonized plant

Plant pathogens but not antagonists change in soil fungal communities across a land abandonment gradient in a Mediterranean landscape

We assessed whether the presence and abundance of plant pathogens and antagonists change in soil fungal communities along a land abandonment gradient. The study was carried out in the Cilento area (Southern Italy) at a site with three different habitats found along a land abandonment gradient: agricultural land, Mediterranean shrubland and woodland. For all microbiological substrates the colony forming units were about 3.1 × 106 g−1 soil for agricultural land and about 1.1 × 106 g−1 soil for Mediterranean shrubland and woodland. We found the following genera in all habitats: Cladosporium, Mortierella, Penicillium and Trichoderma. In agricultural land, the significantly most abundant fungus genera were Aspergillus, Fusarium, Cylindrocarpon and Nectria; in Mediterranean shrubland, Rhizopus and Trichoderma; and in woodland, Bionectria, Mortierella, Cladosporium, Diplodia, Paecilomyces, Penicillium and Trichoderma. We found a total of 8, 8 and 9 species of fungal antagonist, and 16, 6 and 6 species of fungal plant pathogens in agricultural land, Mediterranean shrubland and woodland respectively. Fungal plant pathogens decreased significantly over a land abandonment gradient, while we no found significant differences among fungal antagonists in the three habitats. We conclude that a decrease in the number of fungal pathogen species occurs when formerly cultivated areas are abandoned. On the other hand, fungal antagonists seem not to be affected by this process

Severe outbreak of Fusarium solani on Quercus ilex vectored by Xylosandrus compactus

The first European record of an outbreak of Fusarium solani on Quercus ilex following a massive attack by Xylosandrus compactus from southern Italy is reported