52 research outputs found
Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers
Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. & W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae germplasm, as well as that of species belonging to other Arachis sections
Groundnut
Groundnut, a crop rich in nutrients, originated in South America and
spread to the rest of the world. Cultivated groundnut contains a fraction of
the genetic diversity present in their closely related wild relatives, which is
not more than 13 %, due to domestication bottleneck. Closely related ones
are placed in section Arachis , which have not been extensively utilized
until now due to ploidy differences between the cultivated and wild relatives.
In order to overcome Arachis species utilization bottleneck, a large
number of tetraploid synthetics were developed at the Legume Cell
Biology Unit of Grain Legumes Program, ICRISAT, India. Evaluation of
synthetics for some of the constraints showed that these were good sources
of multiple disease and pest resistances. Some of the synthetics were utilized
by developing ABQTL mapping populations, which were screened
for some biotic and abiotic constraints. Phenotyping experiments showed
ABQTL progeny lines with traits of interest necessary for the improvement
of groundnut
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