17 research outputs found
Π‘Π’Π Π£ΠΠ’Π£Π ΠΠ-Π€Π£ΠΠΠ¦ΠΠΠΠΠΠ¬ΠΠ«Π ΠΠΠΠΠΠ ΠΠΠ£Π₯ΠΠΠΠΠ«Π₯ ΠΠΠΠΠΠΠ Π Π ΠΠΠ ΠΠΠΠ’ΠΠ Π’ΠΠ‘Π’-Π‘ΠΠ‘Π’ΠΠ ΠΠΠ― Π ΠΠΠΠΠ ΠΠΠΠΠΠΠ‘Π’ΠΠΠ, ΠΠ ΠΠΠΠΠΠ Π’ΠΠ§ΠΠΠΠ― Π ΠΠΠ’ΠΠΠΠΠΠ¦ΠΠ Π’ΠΠ ΠΠΠΠ ΠΠΠΠΠΠ§ΠΠ‘Π’ΠΠΠΠΠ«Π₯ ΠΠΠΠΠΠΠ ΠΠΠΠΠΠΠΠ
The article discusses results of the structural and functional analysis of molecular genetic abnormalities in various malignant tumors. Investigations have discovered more than 20 new markers for sporadic breast cancer. Several of them formed the test system, allowing the diagnosis with a specificity of 100%. Appearance of TMPRSS2/ERG4 chimeric gene is a frequent tumor-specific event, its expression is correlated with more aggressive forms of prostate cancer, may serve as a molecular marker for tumor cells and androgen assessment of tumor response to hormonal therapy. The effective systems for the early diagnosis of cervix and endometrium cancer were developed as well. Mutations in the VHL, deletions of chromosome 3 and methylation of several genes can predict the course and selection of effective therapy of clear cell kidney cancer. a number of molecular markers were identified for early diagnosis and prognosis of recurrence of bladder cancer. For diagnosis, prognosis and treatment of brain tumors we developed an effective complex system of markers. Protocol of molecular genetics investigation reveals the cause of the disease by more than 90% of patients with retinoblastoma. In order to study abnormal methylation in tumor genomes an innovative technology AFLOAT has been developed that allows to efficiently identify new markers with diagnostic value. Test systems of molecular genetic and epigenetic markers for early diagnosis and prognosis as well as for cancer therapy optimization have shown to be effective, have been approved for use in clinical practice and are being introduced into practical healthcare.Π Π°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ
. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»ΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠΈΡΡ Π±ΠΎΠ»Π΅Π΅ 20 Π½ΠΎΠ²ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² Π΄Π»Ρ ΡΠΏΠΎΡΠ°Π΄ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΠΊΠ° ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ, ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Ρ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌΡ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΡ ΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡΡ 100%. ΠΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ
ΠΈΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π° TMPRSS2/ERG4 ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ°ΡΡΡΠΌ ΠΎΠΏΡΡ
ΠΎΠ»Ρ-ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠΎΠ±ΡΡΠΈΠ΅ΠΌ, Π΅Π³ΠΎ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΊΠΎΡΡΠ΅Π»ΠΈΡΡΠ΅Ρ Ρ Π±ΠΎΠ»Π΅Π΅ Π°Π³ΡΠ΅ΡΡΠΈΠ²Π½ΡΠΌΠΈ ΡΠΎΡΠΌΠ°ΠΌΠΈ ΡΠ°ΠΊΠ° ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΌΠΎΠΆΠ΅Ρ ΡΠ»ΡΠΆΠΈΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌΒ Π°Π½Π΄ΡΠΎΠ³Π΅Π½ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΎΡΠ²Π΅ΡΠ° ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π½Π° Π³ΠΎΡΠΌΠΎΠ½Π°Π»ΡΠ½ΡΡ ΡΠ΅ΡΠ°ΠΏΠΈΡ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ ΡΠΈΡΡΠ΅ΠΌΡ Π΄Π»Ρ ΡΠ°Π½Π½Π΅ΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΡΠ°ΠΊΠ° ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ ΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ. ΠΡΡΠ°ΡΠΈΠΈ Π³Π΅Π½Π° VHL, Π΄Π΅Π»Π΅ΡΠΈΠΈ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 3 ΠΈ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ΄Π° Π³Π΅Π½ΠΎΠ² ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΈ ΠΏΠΎΠ΄Π±ΠΎΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΡΠ²Π΅ΡΠ»ΠΎΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΊΠ° ΠΏΠΎΡΠΊΠΈ. ΠΠ»Ρ ΡΠ°Π½Π½Π΅ΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ, ΠΏΡΠΎΠ³Π½ΠΎΠ·Π° ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ°ΠΊΠ° ΠΌΠΎΡΠ΅Π²ΠΎΠ³ΠΎ ΠΏΡΠ·ΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ ΡΠ΅Π»ΡΠΉ ΡΡΠ΄ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ². ΠΠ»Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ, ΠΏΡΠΎΠ³Π½ΠΎΠ·Π° ΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΠΌΠΎΠ·Π³Π° ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½Π°Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½Π°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ². ΠΡΠΎΡΠΎΠΊΠΎΠ» ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎΒ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΠ±Π½Π°ΡΡΠΆΠΈΡΡ ΠΏΡΠΈΡΠΈΠ½Ρ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Π±ΠΎΠ»Π΅Π΅ ΡΠ΅ΠΌ Ρ 90% ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΡΠ΅ΡΠΈΠ½ΠΎΠ±Π»Π°ΡΡΠΎΠΌΠΎΠΉ. Β ΠΠ»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π°Π½ΠΎΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
Π³Π΅Π½ΠΎΠΌΠ°Ρ
ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΠΈΠ½Π½ΠΎΠ²Π°ΡΠΈΠΎΠ½Π½Π°Ρ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ AFLOAT, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ Π²ΡΡΠ²Π»ΡΡΡ Π½ΠΎΠ²ΡΠ΅ ΠΌΠ°ΡΠΊΠ΅ΡΡ, ΠΈΠΌΠ΅ΡΡΠΈΠ΅ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅. Π’Π΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠΏΠΈΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² Π΄Π»Ρ ΡΠ°Π½Π½Π΅ΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ, ΠΏΡΠΎΠ³Π½ΠΎΠ·Π° ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΈ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ ΡΠ²ΠΎΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ, ΠΏΠΎΠ»ΡΡΠΈΠ»ΠΈ ΡΠ°Π·ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π½Π° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅ ΠΈ Π² Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ Π°ΠΊΡΠΈΠ²Π½ΠΎ Π²Π½Π΅Π΄ΡΡΡΡΡΡ Π² ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π΄ΡΠ°Π²ΠΎΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΠ΅.
Π ΠΠΠ£ΠΠ¬Π’ΠΠ’Π« ΠΠΠΠΠ Π’ΠΠΠ« ΠΠΠ§ΠΠ‘Π’ΠΠ ΠΠΠΠΠ¦ΠΠΠ‘ΠΠΠ ΠΠΠΠΠ©Π ΠΠ Π Π‘ΠΠ§ΠΠ’ΠΠΠΠΠ ΠΠΠΠΠΠΠΠ§ΠΠ-Π‘ΠΠΠΠΠΠΠΠΠΠΠΠΠ Π’Π ΠΠΠΠ
Medical care quality assessment for patients with vertebro-spinal-cord injury at different stages in St.-Petersburg during year was performed. The first aid in the most cases (74,8%) was rendered by emergency service. A vertebro-spinal-cord injuries were not diagnosed by a staff of line and special medical aid brigades in 31,6-51,9%. The causes of incorrect diagnostics at hospital stage: insufficient and delayed patient examination, underestimation of injury severity in consequence of delayed use of neuro-imaging methods. Surgical operations for vertebro-spinal-cord injuries were performed in 59 (8%) cases. 43 (73%) patients needed in vertebral fixation.ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΎΡΠ΅Π½ΠΊΠ° ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠΉ ΠΏΠΎΠΌΠΎΡΠΈ Π½Π° ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π΅Π΅ ΡΡΠ°ΠΏΠ°Ρ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌ Ρ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΠΎΠΉ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΠΎ-ΡΠΏΠΈΠ½Π½ΠΎΠΌΠΎΠ·Π³ΠΎΠ²ΠΎΠΉ ΡΡΠ°Π²ΠΌΠΎΠΉ Π² Π‘Π°Π½ΠΊΡ-ΠΠ΅ΡΠ΅ΡΠ±ΡΡΠ³Π΅ Π·Π° Π³ΠΎΠ΄. ΠΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Ρ ΠΏΠΎΡΡΡΠ°Π΄Π°Π²ΡΠΈΡ
(74,8%) ΠΏΠ΅ΡΠ²Π°Ρ ΠΏΠΎΠΌΠΎΡΡ Π±ΡΠ»Π° ΠΎΠΊΠ°Π·Π°Π½Π° ΡΠ»ΡΠΆΠ±ΠΎΠΉ ΡΠΊΠΎΡΠΎΠΉ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠΉ ΠΏΠΎΠΌΠΎΡΠΈ. ΠΠ° Π΄ΠΎΠ³ΠΎΡΠΏΠΈΡΠ°Π»ΡΠ½ΠΎΠΌ ΡΡΠ°ΠΏΠ΅ ΠΏΠ΅ΡΡΠΎΠ½Π°Π»ΠΎΠΌ Π»ΠΈΠ½Π΅ΠΉΠ½ΡΡ
ΠΈ ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π±ΡΠΈΠ³Π°Π΄ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΠΎ-ΡΠΏΠΈΠ½Π½ΠΎΠΌΠΎΠ·Π³ΠΎΠ²Π°Ρ ΡΡΠ°Π²ΠΌΠ° Π½Π΅ Π±ΡΠ»Π° Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠΎΠ²Π°Π½Π° Π² 31,6-51,9% Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠΉ. ΠΡΠΈΡΠΈΠ½Ρ Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π½Π° Π³ΠΎΡΠΏΠΈΡΠ°Π»ΡΠ½ΠΎΠΌ ΡΡΠ°ΠΏΠ΅ - Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΠ΅ ΠΈ Π½Π΅ΡΠ²ΠΎΠ΅Π²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠ΅ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², Π½Π΅Π΄ΠΎΠΎΡΠ΅Π½ΠΊΠ° ΡΡΠΆΠ΅ΡΡΠΈ ΡΡΠ°Π²ΠΌΡ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΠΈΠΊΠ° ΠΈ ΡΠΏΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, ΡΡΠΎ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ΠΎ ΠΏΠΎΠ·Π΄Π½ΠΈΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π½Π΅ΠΉΡΠΎΠ²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΎΠ½Π½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ². ΠΠΏΠ΅ΡΠ°ΡΠΈΠΈ ΠΏΠΎ ΠΏΠΎΠ²ΠΎΠ΄Ρ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΠΎ-ΡΠΏΠΈΠ½Π½ΠΎΠΌΠΎΠ·Π³ΠΎΠ²ΠΎΠΉ ΡΡΠ°Π²ΠΌΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Ρ Π² 59 (8%) Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡΡ
, ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
Π² 43 (73%) ΠΏΠΎΡΡΠ΅Π±ΠΎΠ²Π°Π»Π°ΡΡ ΡΠΈΠΊΡΠ°ΡΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΠΈΠΊΠ°
DNA methylation in the promoter regions of the laminin family genes in normal and breast carcinoma tissues
Extracellular glycoproteins of the laminin family are essential components of basement membranes involved in a number of biological processes, including tissue differentiation, wound healing, and tumorigenesis. We present the first comprehensive study of promoter methylation status of the genes encoding laminin chains in normal tissues (peripheral blood leucocytes, buccal epithelial cells, autopsy breast tissue samples) and in breast carcinoma samples. Based on the results of this study, we divide laminin genes into three categories. Genes, constitutively methylated in breast tissues include LAMA3A, LAMB2, LAMB3, and LAMC2. Genes prone to abnormal methylation in breast carcinoma include LAMA1, LAMA2, LAMA3B, LAMA4, LAMB1, and LAMC3. Genes that are rarely if ever methylated in breast carcinoma include LAMA5 and LAMC1. The constitutively methylated group includes all of the genes that encode subunits of laminin-5 (the historical name of laminin 332), the promoters of which were previously considered unmethylated in normal tissues and prone to abnormal methylation in breast cancer. Β© 2015, Pleiades Publishing, Inc
DNA methylation in the promoter regions of the laminin family genes in normal and breast carcinoma tissues
Extracellular glycoproteins of the laminin family are essential components of basement membranes involved in a number of biological processes, including tissue differentiation, wound healing, and tumorigenesis. We present the first comprehensive study of promoter methylation status of the genes encoding laminin chains in normal tissues (peripheral blood leucocytes, buccal epithelial cells, autopsy breast tissue samples) and in breast carcinoma samples. Based on the results of this study, we divide laminin genes into three categories. Genes, constitutively methylated in breast tissues include LAMA3A, LAMB2, LAMB3, and LAMC2. Genes prone to abnormal methylation in breast carcinoma include LAMA1, LAMA2, LAMA3B, LAMA4, LAMB1, and LAMC3. Genes that are rarely if ever methylated in breast carcinoma include LAMA5 and LAMC1. The constitutively methylated group includes all of the genes that encode subunits of laminin-5 (the historical name of laminin 332), the promoters of which were previously considered unmethylated in normal tissues and prone to abnormal methylation in breast cancer. Β© 2015, Pleiades Publishing, Inc
Mass spectrometric study of ring-substituted secondary and tertiary Ξ³-aminopiperidines
Fragmentation of secondary and tertiary Ξ³-aminopiperidines proceeds via elimination of Ξ³-amino radicals and the ring substituents of piperidine, and is accompanied by their cleavage. High-resolution mass spectral data, DADI spectra, and fragmentation of deutero analogs confirm this decomposition. On the basis of quantum chemical MNDO calculations the most probable alternate structures have been proposed for a number of typical ions. From the features of dissociative ionization we can determine the kind and location of substituents in the piperidine ring. Β© 1990 Plenum Publishing Corporation
Abnormal hypermethylation of CpG dinucleotides in promoter regions of matrix metalloproteinases genes in breast cancer and its relation to epigenomic subtypes and HER2 overexpression
Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) substantially contribute to the regulation of intercellular interactions and thereby play a role in maintaining the tissue structure and function. We examined methylation of a subset of 5'-cytosine-phosphate-guanine-3' (CpG) dinucleotides in promoter regions of the MMP2, MMP11, MMP14, MMP15, MMP16, MMP17, MMP21, MMP23B, MMP24, MMP25, MMP28, TIMP1, TIMP2, TIMP3, and TIMP4 genes by methylation-sensitive restriction enzyme digestion PCR. In our collection of 183 breast cancer samples, abnormal hypermethylation was observed for CpGs in MMP2, MMP23B, MMP24, MMP25, and MMP28 promoter regions. The non-methylated status of the examined CpGs in promoter regions of MMP2, MMP23B, MMP24, MMP25, and MMP28 in tumors was associated with low HER2 expression, while the group of samples with abnormal hypermethylation of at least two of these MMP genes was significantly enriched with HER2-positive tumors. Abnormal methylation of MMP24 and MMP25 was significantly associated with a CpG island hypermethylated breast cancer subtype discovered by genome-wide DNA bisulfite sequencing. Our results indicate that abnormal hypermethylation of at least several MMP genes promoters is a secondary event not directly functional in breast cancer (BC) pathogenesis. We suggest that it is elevated and/or ectopic expression, rather than methylation-driven silencing, that might link MMPs to tumorigenesis. Β© 2020 by the authors