8 research outputs found
Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population
Aim: To identify clinically significant molecular markers of endometrial cancer. Materials and Methods: Cancer and normal endometrial tissue samples from 20 patients of the Gynecology Clinic of Odessa State Medical University (Odessa, Ukraine) with confirmed endometrial cancer were compared for SSR and ISSR polymorphisms. Identified polymorphic fragments from anonymous genome regions situated between microsatellite repeats underwent direct DNA sequencing; analysis of their homology to sequences from human genome database has been performed. Results: No significant variability for the microsatellite loci adjacent to the E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 and P53 genes was detected. Search for new molecular markers of endometrial cancer within anonymous DNA sequences located between microsatellite repeats revealed 100 bp and 174 bp polymorphic fragments. These fragments were detected correspondingly in 60% and 35% of patients. 100 bp fragment appeared to be homologous to a region within the NFKB gene, 174 bp fragment β to a sequence within the DDR1 gene. Conclusions: NFKB1 and DDR1 genes may be regarded as potential markers for some types of endometrial cancer. This is a first report about possible association of these genes with endometrial cancer.Π¦Π΅Π»Ρ: ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΡΠ°ΠΊΠ° ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΠ΅ΡΠΎΠ΄Ρ: ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ·
SSR- ΠΈ ISSR-ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ° Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΠΈ Π½Π΅ΠΏΠΎΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ Π΄Π²Π°Π΄ΡΠ°ΡΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π² ΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅ Π³ΠΈΠ½Π΅ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΠ΄Π΅ΡΡΠΊΠΎΠ³ΠΎ Π³ΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΡΠ½ΠΈΠ²Π΅ΡΡΠΈΡΠ΅ΡΠ° (ΠΠ΄Π΅ΡΡΠ°, Π£ΠΊΡΠ°ΠΈΠ½Π°). ΠΡΠΏΠΎΠ»Π½Π΅Π½ΠΎ
ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΡΡ
ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΡ
ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ² ΠΠΠ, Π»ΠΎΠΊΠ°Π»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΡ
Π² Π°Π½ΠΎΠ½ΠΈΠΌΠ½ΡΡ
ΡΡΠ°ΡΡΠΊΠ°Ρ
Π³Π΅Π½ΠΎΠΌΠ° ΠΌΠ΅ΠΆΠ΄Ρ
ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΏΠΎΠ²ΡΠΎΡΠ°ΠΌΠΈ (ISSR-ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌ), ΠΎΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ Π°Π½Π°Π»ΠΈΠ· ΠΈΡ
Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΠΈ Ρ ΠΈΠ·Π²Π΅ΡΡΠ½ΡΠΌΠΈ ΡΡΠ°ΡΡΠΊΠ°ΠΌΠΈ ΠΠΠ ΠΈΠ·
Π±Π°Π·Ρ Π΄Π°Π½Π½ΡΡ
Π³Π΅Π½ΠΎΠΌΠ° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π½Π΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ Π²Π°ΡΠΈΠ°Π±Π΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΡ
ΠΏΠΎΠ²ΡΠΎΡΠΎΠ²,
ΡΠΎΡΠ΅Π΄ΡΡΠ²ΡΡΡΠΈΡ
Ρ Π³Π΅Π½Π°ΠΌΠΈ E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 ΠΈ P53. Π ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΏΠΎΠΈΡΠΊΠ°
Π½ΠΎΠ²ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΡΠ°ΠΊΠ° ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ ΡΡΠ΅Π΄ΠΈ Π°Π½ΠΎΠ½ΠΈΠΌΠ½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΠΠ, Π»ΠΎΠΊΠ°Π»ΠΈΠ·ΡΡΡΠΈΡ
ΡΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ
ΠΏΠΎΠ²ΡΠΎΡΠ°ΠΌΠΈ, Π²ΡΡΠ²Π»Π΅Π½Ρ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΠ΅ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΡ Π΄Π»ΠΈΠ½ΠΎΠΉ 100 ΠΈ 174 ΠΏΠ½. ΠΡΠΈ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΡ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π»ΠΈ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ Ρ 60 ΠΈ
35% ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² . Π€ΡΠ°Π³ΠΌΠ΅Π½Ρ Π΄Π»ΠΈΠ½ΠΎΠΉ 100 ΠΏΠ½ ΠΎΠΊΠ°Π·Π°Π»ΡΡ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠΌ ΡΡΠ°ΡΡΠΊΡ Π³Π΅Π½Π° NFKB1, Π° ΡΡΠ°Π³ΠΌΠ΅Π½Ρ Π΄Π»ΠΈΠ½ΠΎΠΉ 174 ΠΏΠ½ β ΡΡΠ°ΡΡΠΊΡ
Π³Π΅Π½Π° DDR1. ΠΡΠ²ΠΎΠ΄Ρ: Π³Π΅Π½Ρ NFKB1 ΠΈ DDR1 ΠΌΠΎΠ³ΡΡ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΡΠΈΠΏΠΎΠ²
ΡΠ°ΠΊΠ° ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ. ΠΡΠΎ ΠΏΠ΅ΡΠ²ΠΎΠ΅ ΡΠΎΠΎΠ±ΡΠ΅Π½ΠΈΠ΅ ΠΎ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠΉ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ Π΄Π°Π½Π½ΡΡ
Π³Π΅Π½ΠΎΠ² Ρ ΠΎΠΏΡΡ
ΠΎΠ»ΡΠΌΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ
Microarray study of gene expression in uterine leiomyoma
Uterine leiomyoma is a most common benign neoplasm in women of reproductive age. It arises from the myometrial compartment of the uterus and may transform in some cases to a malignant phenotype. Aim: To identify the genes involved in pathogenesis of uterine leiomyoma. Methods: We have studied differential gene expression in matched tissue samples of leiomyoma and normal myometrium from the very same people utilizing a cDNA microarray screening method. We also compared our results with previously published microarray data to identify the overlapping gene alterations. Results: Based on this comparison we can divide genes deregulated in our study into two groups. The first group comprises genes that to our knowledge have not been previously reported as deregulated in fibroids: CLDN1, FGF7 (KGF), HNRPM, ISOC1, MAGEC1 (CT7), MAPK12, RFC, TIE1, TNFRSF21 (DR6). The second group consists of genes identified also in previous studies: CCND1 (BCL1), CDKN1A (P21), CRABP2, FN1 and SOX4 (EVI16). In our study FN1 was the most up-regulated gene, occupying the place between the myometrium and fibroids ranging from 2.07 to 3.64, depending of the probe molecule used for detection. Conclusions: Newly identified genes may be regarded as potential diagnostic or prognostic markers of uterine leiomyoma and thus may be very useful as new therapeutic candidates.ΠΠ΅ΠΉΠΎΠΌΠΈΠΎΠΌΠ° ΠΌΠ°ΡΠΊΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΡ
Π΄ΠΎΠ±ΡΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΉ ΠΆΠ΅Π½ΡΠΊΠΎΠΉ ΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ
ΡΡΠ΅ΡΡ. Π Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΡΠ»ΡΡΠ°ΡΡ
ΠΎΡΠΌΠ΅ΡΠ°ΡΡ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ. Π¦Π΅Π»Ρ: ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ
Π³Π΅Π½ΠΎΠ², Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½Π½ΡΡ
Π² ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π· Π»Π΅ΠΉΠΎΠΌΠΈΠΎΠΌΡ. ΠΠ΅ΡΠΎΠ΄Ρ: ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Π²
ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
Π»Π΅ΠΉΠΎΠΌΠΈΠΎΠΌΡ ΠΈ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΈΠΎΠΌΠ΅ΡΡΠΈΡ ΠΎΠ΄Π½ΠΈΡ
ΠΈ ΡΠ΅Ρ
ΠΆΠ΅ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠ-Π±ΠΈΠΎΡΠΈΠΏ-Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ
ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Ρ Π΄Π°Π½Π½ΡΠΌΠΈ, ΠΎΠΏΡΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΡΠ°Π½Π΅Π΅. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π²ΡΡΠ²Π»Π΅Π½Ρ ΡΠ°Π·Π»ΠΈΡΠΈΡ Π² ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ
ΡΡΠ΄Π° Π³Π΅Π½ΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΠΎΠΆΠ½ΠΎ ΡΠ°Π·Π΄Π΅Π»ΠΈΡΡ Π½Π° Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ. ΠΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π° ΠΏΠΎΠ²ΡΡΠ΅Π½Π½Π°Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ² CLDN1, FGF7 (KGF),
HNRPM, ISOC1, MAGEC1 (CT7), MAPK12, RFC, TIE1 ΠΈ TNFRSF21 (DR6) Π² ΡΠΊΠ°Π½ΠΈ Π»Π΅ΠΉΠΎΠΌΠΈΠΎΠΌΡ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΠΌ
ΠΌΠΈΠΎΠΌΠ΅ΡΡΠΈΠ΅ΠΌ. ΠΠΎ Π²ΡΠΎΡΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΌΠΎΠΆΠ½ΠΎ ΠΎΡΠ½Π΅ΡΡΠΈ Π³Π΅Π½Ρ CCND1 (BCL1), CDKN1A (P21), CRABP2, FN1 ΠΈ SOX4 (EVI16), ΡΠΆΠ΅
ΡΠΏΠΎΠΌΠΈΠ½Π°Π²ΡΠΈΠ΅ΡΡ Π² ΡΠ²ΡΠ·ΠΈ Ρ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·ΠΎΠΌ Π»Π΅ΠΉΠΎΠΌΠΈΠΎΠΌΡ Π² ΡΡΠ΄Π΅ ΠΏΡΠ΅Π΄ΡΠ΄ΡΡΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ. ΠΠ°ΠΈΠ±ΠΎΠ»ΡΡΠΈΠΌ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ
(Π² 2,07β3,64 ΡΠ°Π· Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ Π·ΠΎΠ½Π΄Π°) Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π»ΡΡ Π³Π΅Π½ ΡΠΈΠ±ΡΠΎΠ½Π΅ΠΊΡΠΈΠ½Π° FN1. ΠΡΠ²ΠΎΠ΄Ρ: ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅
Π³Π΅Π½Ρ ΠΌΠΎΠ³ΡΡ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ
Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² Π»Π΅ΠΉΠΎΠΌΠΈΠΎΠΌΡ ΠΌΠ°ΡΠΊΠΈ
Synthis and Phisical And Chemical; Properties of SiO[2]-B[2]O[3] and SiO[2]-P[2]O[5] Thin Film Systems and Powders
The SiO[2]-B[2]O[3] and SiO[2]-P[2]O[5] films were synthesized by using film forming solutions having a P[2]O[5] content of up to 30% and B[2]O[3] up to 40%. Properties of the filmforming solutions and binary oxides were examined. The physical and chemical processes occurring in the solution during the heat treatment of films were examined. The conditions for producing films of different thicknesses were determined. The kinetic parameters were calculated