50 research outputs found
Molecular cloning and transcriptional regulation of ompT , a ToxR-repressed gene in Vibrio cholerae
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72189/1/j.1365-2958.2000.01699.x.pd
Use of race and cultivar-specific elicitors from intercellular fluids for characterizing races of Cladosporium fulvum and resistant tomato cultivars
The Vibrio cholerae ToxR/TcpP/ToxT virulence cascade: distinct roles for two membrane-localized transcriptional activators on a single promoter
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75237/1/j.1365-2958.2000.02111.x.pd
Influence of bioengineered skin substitutes on diabetic foot ulcer and venous leg ulcer outcomes
This systematic review indicates that bioengineered skin substitutes with a dermal component may improve healing outcomes in diabetic foot ulcers and venous leg ulcers. However, better designed trials with longer follow-up periods are needed.C. Barber, A. Watt, C. Pham, K. Humphreys, A. Penington, K. Mutimer, M. Edwards and G. Madder
Analysis of membrane protein interaction: ToxR can dimerize the amino terminus of phage lambda repressor
Regulation of gene expression in Vibrio cholerae by ToxT involves both antirepression and RNA polymerase stimulation
Co-ordinate expression of many virulence genes in the human pathogen Vibrio cholerae is under the direct control of the ToxT protein, including genes whose products are required for the biogenesis of the toxin-co-regulated pilus (TCP) and cholera toxin (CTX). This work examined interactions between ToxT and the promoters of ctx and tcpA genes. We found that a minimum of three direct repeats of the sequence TTTTGAT is required for ToxT-dependent activation of the ctx promoter, and that the region from –85 to –41 of the tcpA promoter contains elements that are responsive to ToxT-dependent activation. The role of H-NS in transcription of ctx and tcpA was also analysed. The level of activation of ctx–lacZ in an E. coli hns – strain was greatly increased even in the absence of ToxT, and was further enhanced in the presence of ToxT. In contrast, H-NS plays a lesser role in the regulation of the tcpA promoter. Electrophoretic mobility shift assays demonstrated that 6 × His-tagged ToxT directly, and specifically, interacts with both the ctx and tcpA promoters. DNase I footprinting analysis suggests that there may be two ToxT binding sites with different affinity in the ctx promoter and that ToxT binds to –84 to –41 of the tcpA promoter. In vitro transcription experiments demonstrated that ToxT alone is able to activate transcription from both promoters. We hypothesize that under conditions appropriate for ToxT-dependent gene expression, ToxT binds to AT-rich promoters that may have a specific secondary conformation, displaces H-NS and stimulates RNA polymerase resulting in transcription activation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71500/1/j.1365-2958.2002.02721.x.pd