Conservation of grape genetic resources in the system in vitro

Conservation of grape genetic resources in the system in vitro can be a contribution to ampelographic collections, and a source of their replenishment with healthy planting material. The goal of the study is to create a vegetative in vitro collection of plants of promising grape varieties and clones, optimize the conditions for maintaining samples. The basis for obtaining, cultivating, clonal micro-propagating of grape plants is the research and development of the Institute Magarach. At the moment, the collection is represented by 144 samples of grape varieties and clones. Samples of the collection are kept in a state of active growth, slow growth and true dormancy. Previous studies followed to establishment of five modes to maintain grape plants in the system in vitro. Changes in the parameters of individual factors affecting morphogenesis made it possible to slow down growth processes and reduce the number of re-plantings. The most promising mode to conserve samples is the fifth one (this method is patented). As a result of the research carried out in the Institute Magarach, a vegetative collection of plants in vitro of promising grape varieties and clones was created. It includes Crimean autochthonous varieties and varieties of the Institute Magarach breeding. The developed modes of collection conservation allow slowing down growth processes and maintaining plants in a state of true dormancy without re-planting for one to two years

Features of somatic embryogenesis in the culture

Correlation analysis of the influence of media variants with introducing various biologically active substances on the development of somatic embryoids and germinating seedlings in the hybrid form E-342 was carried out during two repeated subculturings on the same variant of media, and during the third subculturing - on different variants of media. The repeated subculturing in media with the same growth regulator BAP or IAA caused the development of abnormal large somatic embryoids without the expressed hypocotyls and cotyledons. Only the use of medium variant with DLPA in two subculturings (the third subculturing into the medium with BAP or IAA) and the third repeated subculturing with DLPA, but to a smaller extent, have led to the development of torpedo-shaped embryoids, to be growing into germinating seedlings. Strong positive correlation dependence of influence was established for: BAP on the development of heart-shaped embryoids; IAA on the increase in the size of globular embryoids and transformation of heart-shaped embryoids into torpedo-shaped ones; DLPA on the development of a great number of normal torpedo-shaped embryoids. After longstanding culturing of suspensions of cells and embryoids, it was not possible to get rid of chlorophyll deficiency in the resulting germinating seedlings due to somaclonal variability

Synthesis, spectroscopic characterization, X-ray structure, and in vivo neurotropic activity of new 1,5-benzodiazepin-2-ones

The paper reports the synthesis and in vivo pharmacological studies of a series of N-alkyl-1,5-benzodiazepine-2-ones. In this work, 19 novel benzodiazepine derivatives have been prepared and characterized by spectroscopic methods including 2D nuclear magnetic resonance techniques. Crystal structure of 1-benzyl-8-methyl-4-phenyl-1H-benzo[b][1,4]diazepin-2(3H)-one has also been determined by X-ray diffraction. Prediction of activity spectra for substances prediction and docking studies onto human serum albumin were conducted. Two compounds under these investigation showed high antihypoxic, tranquilizing, and anticonvulsant activity in vivo. © 2016, Springer Science+Business Media New York

Synthesis, spectroscopic characterization, X-ray structure, and in vivo neurotropic activity of new 1,5-benzodiazepin-2-ones

The paper reports the synthesis and in vivo pharmacological studies of a series of N-alkyl-1,5-benzodiazepine-2-ones. In this work, 19 novel benzodiazepine derivatives have been prepared and characterized by spectroscopic methods including 2D nuclear magnetic resonance techniques. Crystal structure of 1-benzyl-8-methyl-4-phenyl-1H-benzo[b][1,4]diazepin-2(3H)-one has also been determined by X-ray diffraction. Prediction of activity spectra for substances prediction and docking studies onto human serum albumin were conducted. Two compounds under these investigation showed high antihypoxic, tranquilizing, and anticonvulsant activity in vivo. © 2016, Springer Science+Business Media New York