Detection of aptamer-protein interactions using QCM and electrochemical indicator methods

Electrochemical indicator methylene blue and differential pulse voltammetry allowing to determine charge transfer from electrode surface to the thrombin bounded on a DNA aptamer and thus to detect thrombin (1) with high selectivity in comparison with nonspecific binding caused by human IgG (2) or human serum albumin (HSA) (3). We report novel method of detection thrombin-aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity. © 2004 Elsevier Ltd. All rights reserved

Detection of aptamer-protein interactions using QCM and electrochemical indicator methods

Electrochemical indicator methylene blue and differential pulse voltammetry allowing to determine charge transfer from electrode surface to the thrombin bounded on a DNA aptamer and thus to detect thrombin (1) with high selectivity in comparison with nonspecific binding caused by human IgG (2) or human serum albumin (HSA) (3). We report novel method of detection thrombin-aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity. © 2004 Elsevier Ltd. All rights reserved

Detection of aptamer-protein interactions using QCM and electrochemical indicator methods

Electrochemical indicator methylene blue and differential pulse voltammetry allowing to determine charge transfer from electrode surface to the thrombin bounded on a DNA aptamer and thus to detect thrombin (1) with high selectivity in comparison with nonspecific binding caused by human IgG (2) or human serum albumin (HSA) (3). We report novel method of detection thrombin-aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity. © 2004 Elsevier Ltd. All rights reserved

Predicting induced radioactivity in a large high-energy physics apparatus: The example of the ATLAS experiment

Calculations of induced radioactivity in the ATLAS detector were performed in order to define its radioactive waste zoning. Two independent studies were carried out. The first was based on folding particle flux spectra obtained from the GCALOR code with the radionuclide production cross-sections. The second study was based on extensive calculations with the FLUKA code. This paper describes the two studies in detail; it presents the calculation methods and discusses their advantages and disadvantages. A comparison of their predictions is given. Finally, the paper discusses the designation of the ATLAS radioactive waste zoning on the basis of the combined knowledge gained from both studies. (C) 2008 Elsevier B.V. All rights reserved