7 research outputs found

    Π‘omparative tests of differentiated fertilizer application for wheat using task cards and nitrogen scanner

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    In the training and experimental farm of the Kuban State Agrarian University Krasnodarskoe from February 19 to July 6, 2020, an experiment was conducted on the comparative analysis of differentiated fertilizer application in on-line and off-line modes. The aim of the experiment was to compare the modes of differentiated application of nitrogen fertilizers (first and second top dressing) for winter wheat using task maps and GreenSeeker sensors. The calculation of economic efficiency showed that when using nitrogen scanners, fertilizers are saved on average 16 kg / ha, compared to the mode of creating task maps based on the NDVI index based on satellite data without losing grain yield and quality (gluten content increased by 2.3 %; protein content-0.6 %)

    Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma

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    Aim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development. Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was assessed by determination of specific antibodies to the endotoxin of A, M, G classes and sCD14 by ELISA. The polymorphism was analyzed by the allele-specific polymerase chain reaction with electrophoretic detection. It was estimated that the risk of early-onset asthma in the population of Crimea is associated with genotypes AG and GG (Asp299Gly) of TLR-4. There were increased levels of anti-endotoxin IgM and decreased of sIgA in patients with late-onset asthma and AA genotype as compared to other genotypes. Conclusion. The gene polymorphism of Asp299Gly TLR-4 is associated with the risk of early-onset asthma development in Crimea population

    Π†ΠΌΡƒΠ½Ρ–Ρ‚Π΅Ρ‚ Π΄ΠΎ Сндотоксину Ρ‚Π° Asp299Gly ΠΏΠΎΠ»Ρ–ΠΌΠΎΡ€Ρ„Ρ–Π·ΠΌ Π³Π΅Π½Π° TLR-4 Ρƒ дорослих Ρ…Π²ΠΎΡ€ΠΈΡ… Π½Π° Π±Ρ€ΠΎΠ½Ρ…Ρ–Π°Π»ΡŒΠ½Ρƒ астму Π· Ρ€Π°Π½Π½Ρ–ΠΌ Ρ– ΠΏΡ–Π·Π½Ρ–ΠΌ ΠΏΠΎΡ‡Π°Ρ‚ΠΊΠΎΠΌ

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    Aim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development.Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was assessed by determination of specific antibodies to the endotoxin of A, M, G classes and sCD14 by ELISA. The polymorphism was analyzed by the allele-specific polymerase chain reaction with electrophoretic detection. It was estimated that the risk of early-onset asthma in the population of Crimea is associated with genotypes AG and GG (Asp299Gly) of TLR-4. There were increased levels of anti-endotoxin IgM and decreased of sIgA in patients with late-onset asthma and AA genotype as compared to other genotypes.Conclusion. The gene polymorphism of Asp299Gly TLR-4 is associated with the risk of early-onset asthma development in Crimea population.ΠŸΠΎΠ»ΠΈΠΌΠΎΡ€Ρ„ΠΈΠ·ΠΌ Asp299Gly Π³Π΅Π½Π° TLR-4 ΠΌΠΎΠΆΠ΅Ρ‚ Π±Ρ‹Ρ‚ΡŒ связан с риском возникновСния Π±Ρ€ΠΎΠ½Ρ…ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ астмы. Π‘ Ρ†Π΅Π»ΡŒΡŽ изучСния ΠΏΠΎΠ»ΠΈΠΌΠΎΡ€Ρ„ΠΈΠ·ΠΌΠ° Asp299Gly Π³Π΅Π½Π° TLR-4 ΠΈ состояния антиэндотоксинового ΠΈΠΌΠΌΡƒΠ½ΠΈΡ‚Π΅Ρ‚Π° исслСдовано 262 ΠΏΠ°Ρ†ΠΈΠ΅Π½Ρ‚ΠΎΠ² с Ρ€Π°Π½Π½ΠΈΠΌ ΠΈ 69 c ΠΏΠΎΠ·Π΄Π½ΠΈΠΌ Π½Π°Ρ‡Π°Π»ΠΎΠΌ астмы. БостояниС антиэндотоксинового ΠΈΠΌΠΌΡƒΠ½ΠΈΡ‚Π΅Ρ‚Π° ΠΎΡ†Π΅Π½ΠΈΠ²Π°Π»ΠΈ с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ спСцифичСских антиэндотоксиновых Π°Π½Ρ‚ΠΈΡ‚Π΅Π» классов A, M, G ΠΈ sCD14 ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. Для ΠΎΡ†Π΅Π½ΠΊΠΈ ΠΏΠΎΠ»ΠΈΠΌΠΎΡ€Ρ„ΠΈΠ·ΠΌΠ° использовали ΠΌΠ΅Ρ‚ΠΎΠ΄ аллСль-спСцифичСской ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€Π°Π·Π½ΠΎΠΉ Ρ†Π΅ΠΏΠ½ΠΎΠΉ Ρ€Π΅Π°ΠΊΡ†ΠΈΠΈ с элСктрофорСтичСской Π΄Π΅Ρ‚Π΅ΠΊΡ†ΠΈΠ΅ΠΉ. УстановлСно, Ρ‡Ρ‚ΠΎ риск развития астмы Ρ€Π°Π½Π½Π΅Π³ΠΎ Π½Π°Ρ‡Π°Π»Π° Π² популяции АР ΠšΡ€Ρ‹ΠΌ связан с Π³Π΅Π½ΠΎΡ‚ΠΈΠΏΠ°ΠΌΠΈ АG ΠΈ GG ΠΏΠΎΠ»ΠΈΠΌΠΎΡ€Ρ„Π½ΠΎΠ³ΠΎ участка Asp299Gly Π³Π΅Π½Π° TLR-4. Π£ ΠΏΠ°Ρ†ΠΈΠ΅Π½Ρ‚ΠΎΠ² с астмой ΠΏΠΎΠ·Π΄Π½Π΅Π³ΠΎ Π½Π°Ρ‡Π°Π»Π° для Π³Π΅Π½ΠΎΡ‚ΠΈΠΏΠ° АА ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с AG Π½Π°Π±Π»ΡŽΠ΄Π°Π΅Ρ‚ΡΡ ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΠ΅ уровня антиэндотоксинового IgM ΠΈ сниТСниС уровня sIgA.ΠŸΠΎΠ»Ρ–ΠΌΠΎΡ€Ρ„Ρ–Π·ΠΌ Asp299Gly Π³Π΅Π½Π° TLR-4 ΠΌΠΎΠΆΠ΅ Π±ΡƒΡ‚ΠΈ ΠΏΠΎΠ²'язаний Ρ–Π· Ρ€ΠΈΠ·ΠΈΠΊΠΎΠΌ виникнСння Π±Ρ€ΠΎΠ½Ρ…Ρ–Π°Π»ΡŒΠ½ΠΎΡ— астми. Π— ΠΌΠ΅Ρ‚ΠΎΡŽ вивчСння ΠΏΠΎΠ»Ρ–ΠΌΠΎΡ€Ρ„Ρ–Π·ΠΌΡƒ Asp299Gly Π³Π΅Π½Π° TLR-4 Ρ– стану антиСндотоксинового Ρ–ΠΌΡƒΠ½Ρ–Ρ‚Π΅Ρ‚Ρƒ дослідили 262 ΠΏΠ°Ρ†Ρ–Ρ”Π½Ρ‚Ρ–Π² Ρ–Π· Ρ€Π°Π½Π½Ρ–ΠΌ Ρ– 69 Ρ–Π· ΠΏΡ–Π·Π½Ρ–ΠΌ ΠΏΠΎΡ‡Π°Ρ‚ΠΊΠΎΠΌ астми. Π‘Ρ‚Π°Π½ антиСндотоксинового Ρ–ΠΌΡƒΠ½Ρ–Ρ‚Π΅Ρ‚Ρƒ ΠΎΡ†Ρ–Π½ΡŽΠ²Π°Π»ΠΈ Π·Π° допомогою визначСння спСцифічних антиСндотоксинових Π°Π½Ρ‚ΠΈΡ‚Ρ–Π» класів A, M, G Ρ– sCD14 ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ Ρ–ΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»Ρ–Π·Ρƒ. Для ΠΎΡ†Ρ–Π½ΡŽΠ²Π°Π½Π½Ρ ΠΏΠΎΠ»Ρ–ΠΌΠΎΡ€Ρ„Ρ–Π·ΠΌΡƒ використовували ΠΌΠ΅Ρ‚ΠΎΠ΄ алСль-спСцифічної ΠΏΠΎΠ»Ρ–ΠΌΠ΅Ρ€Π°Π·Π½ΠΎΡ— Π»Π°Π½Ρ†ΡŽΠ³ΠΎΠ²ΠΎΡ— Ρ€Π΅Π°ΠΊΡ†Ρ–Ρ— Π· Π΅Π»Π΅ΠΊΡ‚Ρ€ΠΎΡ„ΠΎΡ€Π΅Ρ‚ΠΈΡ‡Π½ΠΎΡ— Π΄Π΅Ρ‚Π΅ΠΊΡ†Ρ–Ρ”ΡŽ. Встановили, Ρ‰ΠΎ Ρ€ΠΈΠ·ΠΈΠΊ Ρ€ΠΎΠ·Π²ΠΈΡ‚ΠΊΡƒ астми Ρ€Π°Π½Π½ΡŒΠΎΠ³ΠΎ ΠΏΠΎΡ‡Π°Ρ‚ΠΊΡƒ Π² популяції АР ΠšΡ€ΠΈΠΌ ΠΏΠΎΠ²'язаний Ρ–Π· Π³Π΅Π½ΠΎΡ‚ΠΈΠΏΠ°ΠΌΠΈ АG Ρ– GG ΠΏΠΎΠ»Ρ–ΠΌΠΎΡ€Ρ„Π½ΠΎΡ— ділянки Asp299Gly Π³Π΅Π½Π° TLR-4. Π£ ΠΏΠ°Ρ†Ρ–Ρ”Π½Ρ‚Ρ–Π² Π· Π°ΡΡ‚ΠΌΠΎΡŽ ΠΏΡ–Π·Π½ΡŒΠΎΠ³ΠΎ ΠΏΠΎΡ‡Π°Ρ‚ΠΊΡƒ для Π³Π΅Π½ΠΎΡ‚ΠΈΠΏΡƒ АА Π² порівнянні Π· AG ΡΠΏΠΎΡΡ‚Π΅Ρ€Ρ–Π³Π°Ρ”Ρ‚ΡŒΡΡ Π·Π±Ρ–Π»ΡŒΡˆΠ΅Π½Π½Ρ рівня антиСндотоксинових IgM Ρ– зниТСння рівня sIgA
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