7 research outputs found
Π‘omparative tests of differentiated fertilizer application for wheat using task cards and nitrogen scanner
In the training and experimental farm of the Kuban State Agrarian University Krasnodarskoe from February 19 to July 6, 2020, an experiment was conducted on the comparative analysis of differentiated fertilizer application in on-line and off-line modes. The aim of the experiment was to compare the modes of differentiated application of nitrogen fertilizers (first and second top dressing) for winter wheat using task maps and GreenSeeker sensors. The calculation of economic efficiency showed that when using nitrogen scanners, fertilizers are saved on average 16 kg / ha, compared to the mode of creating task maps based on the NDVI index based on satellite data without losing grain yield and quality (gluten content increased by 2.3 %; protein content-0.6 %)
Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma
Aim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development.
Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was assessed by determination of specific antibodies to the endotoxin of A, M, G classes and sCD14 by ELISA. The polymorphism was analyzed by the allele-specific polymerase chain reaction with electrophoretic detection. It was estimated that the risk of early-onset asthma in the population of Crimea is associated with genotypes AG and GG (Asp299Gly) of TLR-4. There were increased levels of anti-endotoxin IgM and decreased of sIgA in patients with late-onset asthma and AA genotype as compared to other genotypes.
Conclusion. The gene polymorphism of Asp299Gly TLR-4 is associated with the risk of early-onset asthma development in Crimea population
ΠΠΌΡΠ½ΡΡΠ΅Ρ Π΄ΠΎ Π΅Π½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½Ρ ΡΠ° Asp299Gly ΠΏΠΎΠ»ΡΠΌΠΎΡΡΡΠ·ΠΌ Π³Π΅Π½Π° TLR-4 Ρ Π΄ΠΎΡΠΎΡΠ»ΠΈΡ Ρ Π²ΠΎΡΠΈΡ Π½Π° Π±ΡΠΎΠ½Ρ ΡΠ°Π»ΡΠ½Ρ Π°ΡΡΠΌΡ Π· ΡΠ°Π½Π½ΡΠΌ Ρ ΠΏΡΠ·Π½ΡΠΌ ΠΏΠΎΡΠ°ΡΠΊΠΎΠΌ
Aim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development.Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was assessed by determination of specific antibodies to the endotoxin of A, M, G classes and sCD14 by ELISA. The polymorphism was analyzed by the allele-specific polymerase chain reaction with electrophoretic detection. It was estimated that the risk of early-onset asthma in the population of Crimea is associated with genotypes AG and GG (Asp299Gly) of TLR-4. There were increased levels of anti-endotoxin IgM and decreased of sIgA in patients with late-onset asthma and AA genotype as compared to other genotypes.Conclusion. The gene polymorphism of Asp299Gly TLR-4 is associated with the risk of early-onset asthma development in Crimea population.ΠΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌ Asp299Gly Π³Π΅Π½Π° TLR-4 ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ²ΡΠ·Π°Π½ Ρ ΡΠΈΡΠΊΠΎΠΌ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΡ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΡ. Π‘ ΡΠ΅Π»ΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ° Asp299Gly Π³Π΅Π½Π° TLR-4 ΠΈ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ Π°Π½ΡΠΈΡΠ½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ 262 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΡΠ°Π½Π½ΠΈΠΌ ΠΈ 69 c ΠΏΠΎΠ·Π΄Π½ΠΈΠΌ Π½Π°ΡΠ°Π»ΠΎΠΌ Π°ΡΡΠΌΡ. Π‘ΠΎΡΡΠΎΡΠ½ΠΈΠ΅ Π°Π½ΡΠΈΡΠ½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ° ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π°Π½ΡΠΈΡΠ½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΡΡ
Π°Π½ΡΠΈΡΠ΅Π» ΠΊΠ»Π°ΡΡΠΎΠ² A, M, G ΠΈ sCD14 ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. ΠΠ»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ Π°Π»Π»Π΅Π»Ρ-ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Ρ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄Π΅ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΡΠΈΡΠΊ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π°ΡΡΠΌΡ ΡΠ°Π½Π½Π΅Π³ΠΎ Π½Π°ΡΠ°Π»Π° Π² ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ ΠΠ ΠΡΡΠΌ ΡΠ²ΡΠ·Π°Π½ Ρ Π³Π΅Π½ΠΎΡΠΈΠΏΠ°ΠΌΠΈ ΠG ΠΈ GG ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΡΡΠΊΠ° Asp299Gly Π³Π΅Π½Π° TLR-4. Π£ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π°ΡΡΠΌΠΎΠΉ ΠΏΠΎΠ·Π΄Π½Π΅Π³ΠΎ Π½Π°ΡΠ°Π»Π° Π΄Π»Ρ Π³Π΅Π½ΠΎΡΠΈΠΏΠ° ΠΠ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ AG Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ Π°Π½ΡΠΈΡΠ½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ IgM ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ sIgA.ΠΠΎΠ»ΡΠΌΠΎΡΡΡΠ·ΠΌ Asp299Gly Π³Π΅Π½Π° TLR-4 ΠΌΠΎΠΆΠ΅ Π±ΡΡΠΈ ΠΏΠΎΠ²'ΡΠ·Π°Π½ΠΈΠΉ ΡΠ· ΡΠΈΠ·ΠΈΠΊΠΎΠΌ Π²ΠΈΠ½ΠΈΠΊΠ½Π΅Π½Π½Ρ Π±ΡΠΎΠ½Ρ
ΡΠ°Π»ΡΠ½ΠΎΡ Π°ΡΡΠΌΠΈ. Π ΠΌΠ΅ΡΠΎΡ Π²ΠΈΠ²ΡΠ΅Π½Π½Ρ ΠΏΠΎΠ»ΡΠΌΠΎΡΡΡΠ·ΠΌΡ Asp299Gly Π³Π΅Π½Π° TLR-4 Ρ ΡΡΠ°Π½Ρ Π°Π½ΡΠΈΠ΅Π½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΌΡΠ½ΡΡΠ΅ΡΡ Π΄ΠΎΡΠ»ΡΠ΄ΠΈΠ»ΠΈ 262 ΠΏΠ°ΡΡΡΠ½ΡΡΠ² ΡΠ· ΡΠ°Π½Π½ΡΠΌ Ρ 69 ΡΠ· ΠΏΡΠ·Π½ΡΠΌ ΠΏΠΎΡΠ°ΡΠΊΠΎΠΌ Π°ΡΡΠΌΠΈ. Π‘ΡΠ°Π½ Π°Π½ΡΠΈΠ΅Π½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΌΡΠ½ΡΡΠ΅ΡΡ ΠΎΡΡΠ½ΡΠ²Π°Π»ΠΈ Π·Π° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ Π²ΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ ΡΠΏΠ΅ΡΠΈΡΡΡΠ½ΠΈΡ
Π°Π½ΡΠΈΠ΅Π½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΠΈΡ
Π°Π½ΡΠΈΡΡΠ» ΠΊΠ»Π°ΡΡΠ² A, M, G Ρ sCD14 ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΡΠ·Ρ. ΠΠ»Ρ ΠΎΡΡΠ½ΡΠ²Π°Π½Π½Ρ ΠΏΠΎΠ»ΡΠΌΠΎΡΡΡΠ·ΠΌΡ Π²ΠΈΠΊΠΎΡΠΈΡΡΠΎΠ²ΡΠ²Π°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ Π°Π»Π΅Π»Ρ-ΡΠΏΠ΅ΡΠΈΡΡΡΠ½ΠΎΡ ΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ°Π·Π½ΠΎΡ Π»Π°Π½ΡΡΠ³ΠΎΠ²ΠΎΡ ΡΠ΅Π°ΠΊΡΡΡ Π· Π΅Π»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅ΡΠΈΡΠ½ΠΎΡ Π΄Π΅ΡΠ΅ΠΊΡΡΡΡ. ΠΡΡΠ°Π½ΠΎΠ²ΠΈΠ»ΠΈ, ΡΠΎ ΡΠΈΠ·ΠΈΠΊ ΡΠΎΠ·Π²ΠΈΡΠΊΡ Π°ΡΡΠΌΠΈ ΡΠ°Π½Π½ΡΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΡΠΊΡ Π² ΠΏΠΎΠΏΡΠ»ΡΡΡΡ ΠΠ ΠΡΠΈΠΌ ΠΏΠΎΠ²'ΡΠ·Π°Π½ΠΈΠΉ ΡΠ· Π³Π΅Π½ΠΎΡΠΈΠΏΠ°ΠΌΠΈ ΠG Ρ GG ΠΏΠΎΠ»ΡΠΌΠΎΡΡΠ½ΠΎΡ Π΄ΡΠ»ΡΠ½ΠΊΠΈ Asp299Gly Π³Π΅Π½Π° TLR-4. Π£ ΠΏΠ°ΡΡΡΠ½ΡΡΠ² Π· Π°ΡΡΠΌΠΎΡ ΠΏΡΠ·Π½ΡΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΡΠΊΡ Π΄Π»Ρ Π³Π΅Π½ΠΎΡΠΈΠΏΡ ΠΠ Π² ΠΏΠΎΡΡΠ²Π½ΡΠ½Π½Ρ Π· AG ΡΠΏΠΎΡΡΠ΅ΡΡΠ³Π°ΡΡΡΡΡ Π·Π±ΡΠ»ΡΡΠ΅Π½Π½Ρ ΡΡΠ²Π½Ρ Π°Π½ΡΠΈΠ΅Π½Π΄ΠΎΡΠΎΠΊΡΠΈΠ½ΠΎΠ²ΠΈΡ
IgM Ρ Π·Π½ΠΈΠΆΠ΅Π½Π½Ρ ΡΡΠ²Π½Ρ sIgA