Comparative analysis of 18S rRNA genes from Myxobolus aeglefini Auerbach, 1906 isolated from cod (Gadus morhua), Plaice (Pleuronectes platessa) and dab (Limanda limanda), using PCR-RFLP

The myxosporean parasite Myxobolus aeglefini is a marine species, which can be found in the cartilage of mainly gadid fish species. The parasite has, however, been recorded in the flatfish plaice (Pleuronectes platessa) and dab (Limanda limanda). It is not clear if isolates from unrelated hosts represent the same species. Therefore a molecular study was conducted to reveal differences at the DNA level between these isolates. PCR was successfully conducted on three different isolates of Myxobolus aeglefini sampled from cod (Gadus morhua), plaice and dab respectively, using 18S rDNA as template. A PCR product of approx. 1600 base pairs was obtained and RFLP (Restriction Fragment Length Polymerase) was conducted on the fragment with the restriction enzymes Hinf I, Msp I and Hae III. No differences between the isolates were found, suggesting that the three isolates represent the same species

MOULDED, PLASTIC PGA PACKAGES

The increase of the size of le chips and the numbers of the pins made it necessary to develop a new kind of package a couple of years ago. PGA packages have appeared and have been used. The authors of this article present a new type of plastic PGA construction. The technology of the package combines the advantages of the production of moulding technologies of packages with PWB. The new plastic PGA package has good electrical and thermal properties and it is cheap. The construction and technology used allow to meet the demands of individual consumers as well - by shortening the time from design to manufacturing. The article describes in detail the testing of the thermal properties of the plastic PGA package

AN INVERSE MARKOV-CHEBYSHEV INEQUALITY

Suppose that X is an arbitrary nonnegative random variable with three given moments E(X), E(X2) and E(X3). Lower bounds will be given for the tail probabilities P(x > a)

Synthesis of Shape-Tailored WO3 Micro-/Nanocrystals and the Photocatalytic Activity of WO3/TiO2 Composites

A traditional semiconductor (WO3) was synthesized from different precursors via hydrothermal crystallization targeting the achievement of three different crystal shapes (nanoplates, nanorods and nanostars). The obtained WO3 microcrystals were analyzed by the means of X-ray diffraction (XRD), scanning electron microscopy (SEM) and diffuse reflectance spectroscopy (DRS). These methods contributed to the detailed analysis of the crystal morphology and structural features. The synthesized bare WO3 photocatalysts were totally inactive, while the P25/WO3 composites were efficient under UV light radiation. Furthermore, the maximum achieved activity was even higher than the bare P25's photocatalytic performance. A correlation was established between the shape of the WO3 crystallites and the observed photocatalytic activity registered during the degradation of different substrates by using P25/WO3 composites

Interplay of drug transporters P-glycoprotein (MDR1), MRP1, OATP1A2 and OATP1B3 in passage of maraviroc across human placenta

Special attention is required when pharmacological treatment is indicated for a pregnant woman. P-glycoprotein (MDR1) is a well-known transporter localized in the maternal blood-facing apical membrane of placental syncytiotrophoblast and is considered to play an important role in protecting the developing fetus. Maraviroc, a MDR1 substrate that is registered for treatment of HIV infection, shows a low toxicity profile, suggesting favorable tolerability also if administered to pregnant women. Nevertheless, there is only poor understanding to date regarding the extent to which it permeates across the placental barrier and what are the transport mechanisms involved. Endeavoring to clarify the passage of maraviroc across placenta, we used in this study the method of closed-circuit perfusion of maraviroc across human placental cotyledon. The data obtained confirmed slight involvement of MDR1, but they also suggest possible interaction with other transport system(s) working in the opposite direction from that of MDR1. Complementary in vitro studies, including cellular experiments on choriocarcinoma BeWo cells as well as transporter-overexpressing MDCKII and A431 cell lines and accumulation in placental fresh villous fragments, revealed maraviroc transport by MRP1, OATP1A2, and OATP1B3 transporters. Based on mRNA expression data in the placental tissue, isolated trophoblasts, and fetal endothelial cells, especially MRP1 and OATP1A2 seem to play a crucial role in cooperatively driving maraviroc into placental tissue. By the example of maraviroc, we show here the important interplay of transporters in placental drug handling and its possibility to overcome the MDR1-mediated efflux. © 2020 The Author