Synthesis of Novel 1,2,3-Thiadiazoles

This work was supported by the Russian Foundation for Basic Research, project 17-03-00641

Surface Plasma Waves Across the Layers of Intrinsic Josephson Junctions

We predict surface electromagnetic waves propagating across the layers of intrinsic Josephson junctions. We find the spectrum of the surface waves and study the distribution of the electromagnetic field inside and outside the superconductor. The profile of the amplitude oscillations of the electric field component of such waves is peculiar: initially, it increases toward the center of the superconductor and, after reaching a crossover point, decreases exponentially.Comment: 11 pages, 5 figure

Resonance effects due to the excitation of surface Josephson plasma waves in layered superconductors

We analytically examine the excitation of surface Josephson plasma waves (SJPWs) in periodically-modulated layered superconductors. We show that the absorption of the incident electromagnetic wave can be substantially increased, for certain incident angles, due to the resonance excitation of SJPWs. The absorption increase is accompanied by the decrease of the specular reflection. Moreover, we find the physical conditions guaranteeing the total absorption (and total suppression of the specular reflection). These conditions can be realized for Bi2212 superconductor films.Comment: 17 pages, 3 figure

Electron Beam Instability in Left-Handed Media

We predict that two electron beams can develop an instability when passing through a slab of left-handed media (LHM). This instability, which is inherent only for LHM, originates from the backward Cherenkov radiation and results in a self-modulation of the beams and radiation of electromagnetic waves. These waves leave the sample via the rear surface of the slab (the beam injection plane) and form two shifted bright circles centered at the beams. A simulated spectrum of radiation has well-separated lines on top of a broad continuous spectrum, which indicates dynamical chaos in the system. The radiation intensity and its spectrum can be controlled either by the beams' current or by the distance between the two beams.Comment: 4 pages, 4 figure

Large temperature dependence of the Casimir force at the metal-insulator transition

The dependence of the Casimir force on material properties is important for both future applications and to gain further insight on its fundamental aspects. Here we derive a general theory of the Casimir force for low-conducting compounds, or poor metals. For distances in the micrometer range, a large variety of such materials is described by universal equations containing a few parameters: the effective plasma frequency, dissipation rate of the free carriers, and electric permittivity in the infrared range. This theory can also describe inhomogeneous composite materials containing small regions with different conductivity. The Casimir force for mechanical systems involving samples made with compounds that have a metal-insulator transition shows an abrupt large temperature dependence of the Casimir force within the transition region, where metallic and dielectric phases coexist.Comment: 23 pages, 9 figure

Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor

The acquisition of new mobile genetic elements contributes to the genetic diversity of Vibrio cholerae strains. An important role in this process belongs to the genetic material obtained from phages. The aim of this work was to identify phage-induced PLE islands in strains of V. cholerae O1 serogroup and to determine the resistance of isolates with and without those mobile genetic elements to the lytic activity of the diagnostic cholera El Tor bacteriophage. Materials and methods. Whole genomes nucleotide sequences of toxigenic and non-toxigenic V. cholerae O1 strains presented in the NCBI GenBank were used for the work. Bioinformatic analysis was performed using the BLAST algorithm, MEGA X (or BioEdit v. 7.0.9.0). The test with phages was carried out according A. Gratia technique. Results and discussion. The analysis of 39 toxigenic strains imported to the territory of the Russian Federation and neighboring countries has revealed one strain of V. cholerae O1 of the classical biovar containing the PLE5 island, and 13 strains of V. cholerae O1 of the El Tor biovar containing the PLE4 island. PLE islands have not been found in non-toxigenic strains. It is shown that strains with PLE4 belong to V. cholerae O1 genovariants of the El Tor biovar and have the ctxB1 gene allele. Isolates with this mobile element caused sporadic cases of the disease in 1994–1999, as well as cholera outbreaks in the Russian Federation (in 1993–1994, in 1998 – Dagestan, and 1993 – Tatarstan) and Ukraine (1994–1995). It has been suggested that, perhaps, the presence of the PLE4 island makes a certain contribution to the resistance of V. cholerae O1 strains of the El Tor biovar to the diagnostic cholera El Tor phage (55.6 % of phage-resistant isolates were detected), but there are other mechanisms that have not yet been identified. Thus, the data on the presence of new mobile genetic elements in the genome of earlier imported toxigenic strains of V. cholerae O1, biovar El Tor have been obtained, which expands information about their genetic organization

Intensity and Trends in Development of Epidemic Process of Ixodes Tick-Borne Borrelioses in the Russian Federation in 2002–2018 and Forecast for 2019

Objective: to analyze the intensity and dynamics of Ixodidae tick-borne borreliosis (ITBB) incidence in Federal Districts and constituent entities of the Russian Federation (RF) in 2002–2018 and give a forecast of epidemic process development for 2019. The maximum number of cases of ITBB in 2002–2018 was registered in the Central Federal District (CFD); further on in a decreasing order, the Volga (VFD), Siberian (SiFD), North-West (NWFD), Ural (UFD), Far Eastern (FEFD), Southern (SoFD), and North Caucasian (NCFD) Federal Districts. The territories are distributed according to the incidence of ITBB, as follows (descending): NWFD, UFD, SiFD, VFD, CFD, FEFD, SoFD, NCFD. When assessing the dynamics of ITBB incidence, a reliable tendency towards decrease in the intensity of the epidemic process was found for the North-West Federal District and the Volga Federal District, in contrast to the Central Federal District, the Southern Federal District and the North-West Federal District, where there is a significant upward trend. For the UFD, the Siberian Federal District, Far Eastern Federal District, and the Russian Federation on the whole the variation in the incidence rates within the confidence intervals of the long-term annual average values is the most likely to be observed in the near future. The constituent entities of the Russian Federation were ranked according to the levels of ITBB morbidity; the trends in epidemic process development, depending upon the degree of epidemic hazard of the territory, were determined. In half of the 26 entities of the Russian Federation, with the average annual incidence rate above 6.5 о /оооо, a reliable trend in epidemic process intensity reduction was revealed. The exception is the Kemerovo Region and the Republic of Tuva, where further increase in ITBB incidence is probable. In the group of 15 entities of the Russian Federation with the average annual incidence of ITBB ranging from 2.9 о /оооо to 6.5 о /оооо, both the tendency towards growth and decrease or absence of a reliable trend in the intensity of the epidemic process is observed with approximately equal frequency. In the group of entities with the average annual incidence rates of ITBB less than 2.9 о /оооо, the increment of the indicator values in the future is highly probable

Test-System for Identification of Typical and Genetically Altered Variants of Cholera Vibrios, Biovar El Tor, Using Real-Time PCR

The aim of the work was to analyze and assess the results of using PCR test-system “V. cholerae variant ctxB-rtxC FL genes” for identification of Vibrio cholerae O1 with differentiation between typical toxigenic and genetically modified variants in a multiplex format with a real-time hybridization-fluorescent recording of results. Materials and methods. To achieve this goal, a set of reagents for the PCR test-system “V. cholerae O1 variant ctxB-rtxC FL genes”, as well as a method for identifying V. cholerae O1 with differentiation between typical toxigenic and genetically altered variants were utilized. The specificity, specific activity and sensitivity of the developed test-system by the example of 35 V. cholerae O1 strains, 6 V. cholerae non-O1 strains, 5 heterologous strains (Shigella zonnei – 2 strains, one Salmonella typhimurium strain, S. enteritidis, Escherichia coli) were investigated. Results and discussion. The panel of reagents for the PCR test system “V. cholerae variant ctxB-rtxC FL genes” detects DNA fragments of the ctxBCl, ctxBEl, rtxC genes in the genome of toxigenic V. cholerae О1 (has specific activity, analytical sensitivity 1·103 GE/ml) and does not detect these genes in non-toxigenic V. cholerae O1 and non-O1, as well as in heterologous strains of microorganisms (100 % specificity). RF Patent No. 2732448 was granted for the PCR test-system “V. cholerae variant ctxB-rtxC FL genes”. It can be used to increase the efficiency of the epidemiological surveillance system and to carry out a justified scope of anti-epidemic measures in the event of cholera importation into the territory of the Russian Federation

Review of Epidemiological Situation on Rickettsioses in the Russian Federation in 2022 as Compared with 2013–2021, Forecast for 2023

The aim of the review was to characterize the epidemiological situation on tick-borne rickettsioses (TR) in the Russian Federation in 2022 in comparison with the pandemic years of 2020–2021 and the pre-pandemic period of 2013–2019, to give the forecast for 2023. During 2013–2022, a pattern was observed in the incidence of rickettsiosis of the tick-borne spotted fever group (with the exception of Astrakhan spotted fever – ASF), that is also characteristic of most other natural focal infections (NFIs): a sharp decrease in registered incidence rates in 2020–2021 with the subsequent increase in 2022 almost up to pre-pandemic levels or even exceedance in some regions. The absence of a defining influence of the frequency of contacts of the population with vectors on the decrease in the incidence in the period of 2020–2021 and the rise in the value in 2022 confirms the registration-related nature of the changes. Given due attention to the problem of effective detection and reporting of NFIs, we expect the incidence of tick-borne rickettsioses to remain within the confidence intervals of long-term average values in most endemic regions in 2023. Relaxed vigilance to the problem of NFIs may lead to a decline in the reported incidence of Siberian tick-borne typhus in the Krasnoyarsk and Zabaikalsky Territories, Republics of Tuva and Khakassia, Novosibirsk and Amur Regions; reduction in the incidence of ASF in Astrakhan Region. The main issue in detecting and registration of the tick-borne rickettsiosis incidence, objective assessment and forecasting of the epidemic situation as regards this group of infections is conditioned by the lack of domestic certified diagnostic test kits for laboratory verification of rickettsioses. Close cooperation between epidemiologists and clinicians is required to establish and register cases of TR based on clinical and epidemiological data

Epidemiological Situation on Tick-Borne Borreliosis in the Russian Federation in 2021 and Forecast for 2022

3875 cases of tick-borne borreliosis (TBB) (2.65 per 100000 of population) were recorded in Russia in 2021. Compared to 2020, 61 out of 78 constituent entities experienced a decrease in the incidence rate in 2021. Over the past year, the largest number of cases was registered in the Central Federal District (CFD) – 1797 cases, which is 46.4 % of cases in Russia. Second in the rank by the number of cases of TBB comes the Siberian FD (SFD) – 616 cases (15.9 %), followed by Ural FD – 445 cases (11.5 %), the North-Western FD – 418 (10.8 %), and the Volga FD – 388 (10 %). 134 (3.5 %) and 60 (1.5 % of the total number of cases of TBB) cases were registered in the Far Eastern and Southern Federal Districts, respectively. The last place is occupied by the North Caucasus Federal District (NCFD), where 17 cases were registered, the share of which in the total structure of cases in Russia is 0.4 %. When assessing the long-term dynamics of TBB incidence, a significant trend towards a decrease in the intensity of the epidemic process has been revealed for the North-Western FD, UFD and VFD, as opposed to the CFD and Southern FD, where a significant upward trend was noted. For the Russian Federation on the whole, the Siberian FD, FEFD and NCFD the variation in the incidence rates within the confidence intervals of the long-term annual average values is most likely to be observed in the near future