РАЗВИТИЕ РЕСПУБЛИКАНСКОЙ ИНФОРМАЦИОННО-ОБРАЗОВАТЕЛЬНОЙ СРЕДЫ

The article is devoted to the formation of a modern educational space. The reasons for the phenomenon DIGITAL TRANSFORMATION, No 3 (4), 2018 47 of digitalization of the education system are analyzed. The characteristics of regulatory and legal provision in the field of information and communication technologies at the international level are given, problems of development and improvement of the quality of electronic educational resources are revealed. In addition, the article considers the structure of the information and educational environment created in the Republic of Belarus, including aspects that go beyond the didactics and management of educational processes. Developed proposals for improving the single information and educational environment, based on the principles of standardization and increased participation in the activities of international organizations.Статья посвящена вопросам формирования современного образовательного пространства. Проанализированы причины возникновения феномена цифровизации системы образования. Дана характеристика нормативно-правового обеспечения в сфере информационно-коммуникационных технологий на международном уровне, выявлены проблемы развития и повышения качества электронных образовательных ресурсов. Кроме того, в статье рассмотрена структура создаваемой в Республике Беларусь информационно-образовательной среды, включающая в том числе аспекты, выходящие за рамки дидактики и управления образовательными процессами. Разработаны предложения по совершенствованию единой информационно-образовательной среды, базирующиеся на принципах стандартизации и расширения участия в деятельности международных организаций

ARTEMIN Promotes De Novo Angiogenesis in ER Negative Mammary Carcinoma through Activation of TWIST1-VEGF-A Signalling

10.1371/journal.pone.0050098PLoS ONE711

Suppression of Her2/neu expression through ILK inhibition is regulated by a pathway involving TWIST and YB-1

In a previous study it was found that the therapeutic effects of QLT0267, a small molecule inhibitor of integrin-linked kinase (ILK), were influenced by Her2/neu expression. To understand how inhibition or silencing of ILK influences Her2/neu expression, Her2/neu signaling was evaluated in six Her2/neu-positive breast cancer cell lines (LCC6Her2, MCF7Her2, SKBR3, BT474, JIMT-1 and KPL-4). Treatment with QLT0267 engendered suppression (32–87%) of total Her2/neu protein in these cells. Suppression of Her2/neu was also observed following small interfering RNA-mediated silencing of ILK expression. Time course studies suggest that ILK inhibition or silencing caused transient decreases in P-AKTser473, which were not temporally related to Her2/neu downregulation. Attenuation of ILK activity or expression was, however, associated with decreases in YB-1 (Y-box binding protein-1) protein and transcript levels. YB-1 is a known transcriptional regulator of Her2/neu expression, and in this study it is demonstrated that inhibition of ILK activity using QLT0267 decreased YB-1 promoter activity by 50.6%. ILK inhibition was associated with changes in YB-1 localization, as reflected by localization of cytoplasmic YB-1 into stress granules. ILK inhibition also suppressed TWIST (a regulator of YB-1 expression) protein expression. To confirm the role of ILK on YB-1 and TWIST, cells were engineered to overexpress ILK. This was associated with a fourfold increase in the level of YB-1 in the nucleus, and a 2- and 1.5-fold increase in TWIST and Her2/neu protein levels, respectively. Taken together, these data indicate that ILK regulates the expression of Her2/neu through TWIST and YB-1, lending support to the use of ILK inhibitors in the treatment of aggressive Her2/neu-positive tumors

Epithelial to mesenchymal transition and breast cancer

Epithelial-mesenchymal plasticity in breast carcinoma encompasses the phenotypic spectrum whereby epithelial carcinoma cells within a primary tumor acquire mesenchymal features and re-epithelialize to form a cohesive secondary mass at a metastatic site. Such plasticity has implications in progression of breast carcinoma to metastasis, and will likely influence response to therapy. The transcriptional and epigenetic regulation of molecular and cellular processes that underlie breast cancer and result in characteristic changes in cell behavior can be monitored using an increasing array of marker proteins. Amongst these markers exists the potential for emergent prognostic, predictive and therapeutic targeting

DEVELOPMENT OF THE REPUBLICAN INFORMATION AND EDUCATIONAL ENVIRONMENT

The article is devoted to the formation of a modern educational space. The reasons for the phenomenon DIGITAL TRANSFORMATION, No 3 (4), 2018 47 of digitalization of the education system are analyzed. The characteristics of regulatory and legal provision in the field of information and communication technologies at the international level are given, problems of development and improvement of the quality of electronic educational resources are revealed. In addition, the article considers the structure of the information and educational environment created in the Republic of Belarus, including aspects that go beyond the didactics and management of educational processes. Developed proposals for improving the single information and educational environment, based on the principles of standardization and increased participation in the activities of international organizations

Control and program complex of pictures processing for the solution of problems of the surface in metallography analysis

In the article there are described the possibilities of the' program Autoscan Objects, intended for stereological analysis of the structured components of different materials. The principles of program creation and carrying out of analysis are shown. There is offered the program Autoscan Areas, intended for measurements of the surfaces, engaged by different phases, and also porosity of the examined materials

Oncogenic role of DDX3 in breast cancer biogenesis

Benzo[a] pyrene diol epoxide (BPDE), the active metabolite of benzo[a] pyrene present in tobacco smoke, is a major cancer-causing compound. To evaluate the effects of BPDE on human breast epithelial cells, we exposed an immortalized human breast cell line, MCF 10A, to BPDE and characterized the gene expression pattern. Of the differential genes expressed, we found consistent activation of DDX3, a member of the DEAD box RNA helicase family. Overexpression of DDX3 in MCF 10A cells induced an epithelial-mesenchymal- like transformation, exhibited increased motility and invasive properties, and formed colonies in soft-agar assays. Besides the altered phenotype, MCF 10A-DDX3 cells repressed E-cadherin expression as demonstrated by both immunoblots and by E-cadherin promoter-reporter assays. In addition, an in vivo association of DDX3 and the E-cadherin promoter was demonstrated by chromatin immunoprecipitation assays. Collectively, these results demonstrate that the activation of DDX3 by BPDE, can promote growth, proliferation and neoplastic transformation of breast epithelial cell