Secured Data Retrieval System in Unfriendly Regions Using CP-ABE Based in Ad Hoc Disruption- Tolerant Networks

This paper mainly focuses on a secure data collection mechanism using CP-ABE for ad hoc DTNs where more than one key authority manages their attributes dynamically and independently. The Military environments like battlefield and hostile networks operate in ad hoc mode and they suffer isolated network connectivity. Deployment of Disruption-tolerant networks (DTN) enhances the connectivity between wireless devices carried by soldiers in battle field; this provides them to communicate effectively and share the information confidently. Cipertext -policy attribute based encryption (CP-ABE) is effective cryptographic technique to access control issues. Ad hoc network are decentralized and resource constrained networks, applying CP-ABE to such networks is a challenging issue, in turn it introduces new security and privacy issues related to attribute revocation, coordination of attributes, and key escrow. The major research issue is to achieve effective and secure communication in Delay-Tolerant Networks by implementing authorization policies and the policies update for secure data collection. We analyzed the proposed mechanism and applied to the disruption-tolerant military network to access the information securely

A COMPARATIVE ANTIBACTERIAL STUDY OF IXORA COCCINEA L. PLANTS WITH RED, ORANGE, PINK AND WHITE FLOWERS

Eradication of pathogenic bacterial infection is the most effective means of curing several diseases and preventingrecurrent episodes. The main objective of this study was to evaluate the comparative antibacterial activity of Ixora coccinea L.plants with red, orange, pink and white flowers against tested bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis,Klebsiella pneumoniae. Both the methanolic and aqueous extracts of all four types of flowers exhibited considerable antibacterialactivities against bacteria. The methanolic extracts of red and pink flowers exhibited high antibacterial activity whereas orangeflowers showed moderate activity while white flowers showed low activity. The aqueous extracts displayed less inhibitory effectscompared to the methanolic extracts. The aqueous extracts of red and pink flowers showed considerable high antibacterialactivity compared to orange and white flowers. The preliminary phytochemical screening revealed the presence of alkaloids,glycosides, steroids, carbohydrates, flavonoids and tannins

BIOSYNTHESIS OF GOLD NANOPARTICLES BY BIOSORPTION USING NEOSARTORYA UDAGAWAE: CHARACTERIZATION AND INVITRO EVALUATION

Objective: The present study was aimed to investigate gold nanoparticles synthesized by fungal isolate Neosartorya udagawae and determination of their stability in biofluids to probe their aptness in drug delivery applications.Methods: In this procedure, gold nanoparticles were prepared by biosynthesis using seven days old culture of Neosartorya udagawae and aqueous chloroauric acid. After the complete reaction, the fungal biomass was subjected to UV-Vis, XRD, FT-IR Spectrum analysis, TEM, Zeta potential, SEM and EDX analysis.Results: Intra/extracellular synthesis of gold nanoparticles was confirmed by a sharp peak at 526 nm in UV spectroscopy. SEM, TEM analysis demonstrates the spherical shape of AuNPs with an average diameter of 50 nm and XRD confirm the crystalline gold nanoparticles. FTIR analysis reveals the presence of the protein shell around the gold nanoparticles. The zeta potential value of AuNPs was-36mV which confirmed the stability of nanoparticles dispersion. Gold nanoparticles have shown high stability in biofluids of Bovine Serum Albumin and Phosphate Buffer Saline at pH-5, pH-7and pH-9 which mimic the human colonic biological environment.Conclusion: The fungal synthesis of AuNPs has been experimentally demonstrated and their stability in BSA, 10% NaCl and PBS at pH-7. This might be a promising option for drug delivery applications in carcinogenic colon disorders in human beings.Â

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Not AvailableThe brown planthopper Nilaparvatalugens (Stål) is a major insect pest that is primarily present in Asia causing a significant impact on rice crop. Seventy-three rice genotypes were evaluated for molecular diversity based on known BPH resistance loci. A total number of 108 alleles were detected by 39 polymorphic markers with an average of 2.37 alleles per locus. The allele frequency which is useful in estimating the frequency of alleles, ranged from 0.054 to 0.9726, while the expected heterozygosity varied between 0.054 (RM494) and 0.688 (RM231) with an average of 0.431.The Polymorphic Information Content (PIC) values provided an estimation of the marker’s discriminating power by ranged from 0.066 (RM496) to 0.621 (RM231) suggesting moderate level of polymorphism for the selected BPH specific SSR markers among the germplasm lines. Accounting the major allele frequency, heterozygosity and PIC content, the marker RM231 associated with QBph3.1 and regarded as the most informative one along with RM19291, RM335, RM469, RM518, RM8213, RM228, RH078 and RM589 for dissecting the molecular diversity of germplasm lines with respect to BPH resistance. Based on the marker data, the genotypes were classified into seven clusters that distinguished the lines clustering as resistant and susceptible separately. The information about the genetic diversity of these lines will be extremely useful for proper selection of parents related to BPH reaction, especially for gene mapping and for marker assisted selection.Not Availabl

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Not AvailableBrown planthopper (BPH), Nilaparvata lugens (Stål) is a destructive pest that poses a threat to the food security of rice producing countries. In order to breed rice varieties with resistance to the brown planthopper, it is necessary to identify highly resistant germplasm sources. Seventy-three genotypes were evaluated using standard seed box screening technique over a period of two seasons that have shown varied levels of response reactions to the planthopper. Simultaneously, fifty-three microsatellite markers linked to BPH resistance were employed to find their association with BPH resistance. This association was estimated using regression based Single Marker Analysis (SMA) in Microsoft Excel. The significant marker trait associations (MTAs) were indicated by a P-value (< 0.05) along with corresponding R2 for each marker explaining the total phenotypic variation for BPH resistance accounted by markers. Statistically significant marker-trait associations were observed for five of the 39 polymorphic markers viz, RM335, RM510, RM589, RM8213 and RM494 indicating the possibility of significant putative genetic loci associated with BPH resistance on chromosomes 4, 6 and 10. The percentage of the total phenotypic variation as explained by the significant markers ranged from 1.0% to 24.4% suggesting the reliability of these genetic markers for further improvement of breeding for BPH resistance. Since these markers are linked with Bph3, Bph17(t), QBph6 and QBph10 genes/QTLs respectively, the presence of these genes in the resistant germplasm lines found in the study could be exploited through marker-assisted breeding programs for the development of BPH resistant cultivars.Not Availabl

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Not AvailableBackground: Rice is the staple food of Indians and it is attacked by more than 100 species of insects. Among all, brown planthopper (BPH), Nilaparvata lugens (Stal) (Hemiptera: Delphacidae) is one of the major sucking insects, which can cause appreciable damage by sucking sap on other hand transmitting viral diseases. Farming community using several insecticides for managing the BPH. However, continuous use of these insecticides causes health hazards and environmental pollution and also leads to development of insecticide resistance. Keeping this view in mind, the present investigation has been planned with combined use of fungal formulations and commonly used neonicotinoid insecticides in rice eco system for managing BPH. Methods: This experiment was carried out in two parts, one is studied the compatibility between neonicotinoids (Thiamethoxam 25 SG and Dinotefuran 20 SG) and entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii =Verticillium lecanii) by following the standard poison food technique. Second one is after studied the compatibility between neonicotinoids and entomopathogenic fungal agents, inhibitory studies were conducted under glasshouse conditions. Result: The study revealed that two tested insecticides affected the radial growth of fungi partially at all the three concentrations (0.5RC, RC and 1.5RC). At recommended concentration (RC), dinotefuran 20 SG @0.4 g/l was found to be slightly harmful to all three fungi (20% - 35% reduction of the growth of the fungus) and thiamethoxam 25 SG @ 0.25g/l appeared to be slightly harmful to B. bassiana and M. anisopliae and moderately harmful to L. lecanii (35-50% reduction of the growth of the fungus). Thiamethoxam 25SG alone recorded 65.0% mortality of BPH and when mixed with B. bassiana and M. anisopliae gave 86.25% and 81.25% mortality of BPH respectively under glass house conditions. Similarly, Dinotefuron 20 SG alone could gave 98.75% mortality of BPH.DST-INSPIR

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Not AvailableFor introgressing Brown planthopper (BPH) resistance into a rice variety using marker assisted selection, study of parental polymorphism between the donor and recipient parents is a pre-requisite step. Unless the parents are polymorphic for the BPH reaction, further selection of plants carrying the traits may not be possible. Thus, the present investigation was conducted to analyze the polymorphism of two BPH resistant donors (M229, 10-3) and a recipient (Telangana Sona) parent using SSR markers. A total of 494 random microsatellite markers evenly distributed across 12 chromosomes were used for the polymorphic survey. Among these, 87 markers were found polymorphic between M229 and Telangana Sona, while 93 were polymorphic between 10-3 and Telangana Sona by producing distinct reproducible amplification patterns. The highest percentage of polymorphism was observed on chromosome 12 (30.59%) for Telangana Sona and M229, while chromosome 1 (30.61%) recorded the highest polymorphism percentage for Telangana Sona and 10-3 indicating that these chromosomes are useful in studying the variation between the parents. Accordingly, the maximum number of polymorphic markers were obtained on chromosome 6 for the parents of both the crosses. The identified polymorphic markers across the 12 chromosomes will be useful in subsequent linkage map construction along with mapping of QTLs associated with BPH resistance.Not Availabl

Rice brown planthopper, Nilaparvata lugens (Stål) feeding behavior in relation to elevated CO2 and temperature

Feeding behavior of brown planthopper, Nilaparvata lugens (Stål) populations collected from different geographical regions Ludhiana, Nalgonda and West Godavari at three generations 1, 5 and 10 fed on rice plants grown under different CO2 and temperature levels 1) Ambient CO2@ 380±25ppm + ambient temperature (aCO2+aT), 2) Elevated CO2@500±25ppm + ambient temperature (eCO2+aT) and 3) Elevated CO2@500±25ppm + elevated temperature (eCO2+eT) in closed CO2 chambers was studied. Elevated CO2 + elevated temperature increased feeding rate of BPH nymphs while BPH adults showed declined feeding rate. BPH nymphal feeding rate increased with progressive generations while it decreased in BPH adults. Ludhiana adult BPH population showed higher honeydew excretion compared to West Godavari and Nalgonda populations. CO2 + temperature levels at progressive generations have varying effects on adults and nymphs of three BPH populations. Biochemical composition of rice plants grown under different CO2 levels revealed increased rate of soluble sugars, phenols and decreased rate of reducing sugars, soluble proteins, free amino acids, nitrogen, potassium, phosphorous in elevated CO2 + elevated temperature levels compared to ambient conditions. Increased feeding rate of BPH under elevated CO2 levels may be to compensate changes in host plant quality i.e., high soluble sugars and low nitrogen