The limit of mass determination with an AFM cantilever-based system

This study was performed in the framework of the Program for Basic Research of State Academies of Sciences for 2013-2020. Shumov I.D. is a recipient of Russian Federation President scholarship for young scientists for 2016-2018 (project identificator SP-4280.2016.4)

Magnetron sputtering deposition of ultra-thin tungsten coatings onto amorphous graphite for enhancement of horseradish peroxidase adsorption

The present study was performed in the framework of the Program for Basic Research of State Academies of Sciences for 2013-2020

Коррекция микробиоценоза, физиологических процессов организма поросят при интенсивной технологии выращивания

The authors presented the results of a study of the feed additive’s effect on the gastrointestinal tract’s microflora, hematological, biochemical, and production indicators of piglets of the technological group 0-1 month of the Large White breed, Landrace, and Duroc. For the experiment, the authors formed two groups of animals - experimental and control. Piglets of the experimental group were fed with Bimulak Pre feed additive from the 2nd to the 15th day of life. Animals of the experimental group were on the usual diet and did not receive the feed additive. As a result of studies, a positive effect of feed additive Bimulak Pre on enterobiocenosis of the gastrointestinal tract of piglets, hematological and biochemical parameters of blood, and production performance. After the application, the feed additive helps increase the amount of beneficial microflora (bifidobacteria and lactobacilli) while reducing lactose-negative variants of Escherichia coli, staphylococci, and yeast-like fungi of the genus Candida in the intestine. Using the analysis of hematological and biochemical parameters, the authors found that the number of erythrocytes and hemoglobin slightly increased in the experimental group of piglets. In addition, the total number of incremental cytes decreases, the total protein content increases, and the glucose level decrease compared to the check. The use of the additive with the essential diet leads to a reduction in the mortality of piglets, increased preservation of young stock, and an increase in average daily live weight gain and average weight at weaning.Представлены результаты исследования влияния кормовой добавки на микрофлору желудочно-кишечного тракта, гематологические, биохимические и производственные показатели поросят технологической группы 0–1 месяц крупной белой породы, ландрас и дюрок. Для проведения эксперимента были сформированы две группы животных – опытная и контрольная. Поросятам опытной группы в рацион вводили кормовую добавку Бимулак Пре со 2-го по 15-й день жизни. Животные опытной группы находились на обычном рационе и кормовую добавку не получали. В результате проведенных исследований установлено положительное влияние кормовой добавки Бимулак Пре на энтеробиоценоз желудочно-кишечного тракта поросят, гематологические и биохимические показатели крови, а также производственные показатели. Кормовая добавка, после курса применения, способствует увеличению количества полезной микрофлоры (бифидо- и лактобактерий) при одновременном уменьшении лактозонегативных вариантов кишечной палочки, стафилококков и дрожжеподобных грибов рода Candida в кишечнике. Анализ гематологических и биохимических показателей показал, что в опытной группе поросят незначительно увеличивается количество эритроцитов и гемоглобина, снижается количество лейкоцитов, возрастает содержание общего белка и уменьшается уровень глюкозы по сравнению с контролем. Применение добавки вместе с основным рационом приводит к снижению падежа поросят, повышению сохранности поголовья молодняка, увеличению среднесуточного прироста живой массы и средней массы при отъеме

VIRUSES OF NEPOVIRUS GENUS (PICORNAVIRALES, SECOVIRIDAE) IN THE SOUTH OF THE FAR EAST: RESULTS OF LONGITUDINAL MONITORING

Abstract. The aim of the presented work consists in the analysis of results of long-term monitoring of the members of one of the most important and interesting phytovirus taxons – genus Nepovirus (Picornavirales, Secoviridae, Comovirinae) – which is carried out since 1962 on the planned basis in the south of the Far East (Primorsky krai, Khabarovsk krai, the Sakhalin region) by the Laboratory of Virology of Federal Scientific Center of the East Asia terrestrial biodiversity of Far Eastern Branch of Russian Academy of Sciences (Vladivostok, Russia). Discussion. Many (it is possible – all) nepoviruses are capable to cause epiphytoties with significant economic damage for world economy. In the current article the organization of nepovirus genome, the principles of their division into three genetic groups (A, B, and C), virion morphology, the principles of division into three beyond density fractions (T, M, and B), the characteristics of phytopathology connected with nepoviruses, their ecology in the south of the Far East (including sources of isolation and vectors) are discussed as well as physical-chemical properties of some the most interesting strains from this region which apparently could represent new species of Nepovirus genus: Phryma asiatica mosaic virus (PhAMV); dayflower mosaic virus (DFMV); foenugreek necrotic spot virus (FoNSV); Capsicum annuum necrotic spot virus (CaNSV). Conclusion. Nepovirus genus is one of the most adapted for circulation in the phytocenoses of midlatitudes having natural reservoir among wild plants and pose serious threat for crops. On the basis of ecological features of nepoviruses the scientifically based list of preventive and protective actions against epiphytoties with nepovirus nature is provided. One of the most important elements of such actions is their planned monitoring at the regional level with obligatory comparison of the received results to universal data

Virus Diseases of Vegetable and Melon Crops in the South of the Russian Far East

Aim. The development of an inventory of phytoviruses affecting vegetable and melon crops in the South of the Russian Far East.Discussion. On the basis of many years of original research, carried out on a regular basis by the Laboratory of Virology (Federal Scientific Centre of East Asia Biodiversity, Far Eastern Branch, Russian Academy of Sciences) since 1962 as well as available data in the literature, information about the symptoms of diseases, circulation, reserve plants and vectors are presented for the alfalfa mosaic virus (Bromoviridae, Alfamovirus); tomato aspermy virus and cucumber mosaic virus (Bromoviridae, Cucumovirus); cauliflower mosaic virus (Caulimoviridae, Caulimovirus); garlic mosaic virus (Potyviridae, Carlavirus); tobacco etch virus, allium yellow dwarf virus, bean yellow mosaic virus, watermelon mosaic virus 2, turnip mosaic virus and bean common mosaic virus (Potyviridae, Potyvirus); radish mosaic virus (Picornavirales, Secoviridae, Comovirus); tobacco ringspot virus (Picornavirales, Secoviridae, Nepo-virus); cucumber green mottle mosaic virus, tobacco mosaic virus and tomato mosaic virus (Virgaviridae, Tobamovirus).Conclusion. The information presented forms the basis for the development of a set of diagnostic test systems for phytovirus diseases of vegetable and melon crops; a necessary element of activities directed to the improvement of food productivity and security of the Russian Federation in the Far East

Bacillus-Based Probiotic Treatment Modified Bacteriobiome Diversity in Duck Feces

The intestinal health of poultry is of great importance for birds’ growth and development; probiotics-driven shifts in gut microbiome can exert considerable indirect effect on birds’ welfare and production performance. The information about gut microbiota of ducks is scarce; by using high throughput metagenomic sequencing with Illumina Miseq we examined fecal bacterial diversity of Peking ducks grown on conventional and Bacillus-probiotic-enriched feed. The probiotic supplementation drastically decreased the presence of the opportunistic pathogen Escherichia/Shigella, which was the major and sole common dominant in all samples. Seventy other bacterial species in the ducks’ fecal assemblages were found to have probiotic-related differences, which were interpreted as beneficial for ducks’ health as was confirmed by the increased production performance of the probiotic-fed ducks. Bacterial α-biodiversity indices increased in the probiotic-fed group. The presented inventory of the duck fecal bacteriobiome can be very useful for the global meta-analysis of similar data in order to gain a better insight into bacterial functioning and interactions with other gut microbiota to improve poultry health, welfare and production performance

Analysis of Single Biomacromolecules and Viruses: Is It a Myth or Reality?

The beginning of the twenty-first century witnessed novel breakthrough research directions in the life sciences, such as genomics, transcriptomics, translatomics, proteomics, metabolomics, and bioinformatics. A newly developed single-molecule approach addresses the physical and chemical properties and the functional activity of single (individual) biomacromolecules and viral particles. Within the alternative approach, the combination of “single-molecule approaches” is opposed to “omics approaches”. This new approach is fundamentally unique in terms of its research object (a single biomacromolecule). Most studies are currently performed using postgenomic technologies that allow the properties of several hundreds of millions or even billions of biomacromolecules to be analyzed. This paper discusses the relevance and theoretical, methodological, and practical issues related to the development potential of a single-molecule approach using methods based on molecular detectors

Nanoribbon Biosensor in the Detection of miRNAs Associated with Colorectal Cancer

A nanoribbon biosensor (NRBS) was developed to register synthetic DNAs that simulate and are analogous to miRNA-17-3p associated with colorectal cancer. Using this nanoribbon biosensor, the ability to detect miRNA-17-3p in the blood plasma of a patient diagnosed with colorectal cancer has been demonstrated. The sensing element of the NRBS was a nanochip based on a silicon-on-insulator (SOI) nanostructure. The nanochip included an array of 10 nanoribbons and was designed with the implementation of top-down technology. For biospecific recognition of miRNA-17-3p, the nanochip was modified with DNA probes specific for miRNA-17-3p. The performance of the nanochip was preliminarily tested on model DNA oligonucleotides, which are synthetic analogues of miRNA-17-3p, and a detection limit of ~10−17 M was achieved. The results of this work can be used in the development of serological diagnostic systems for early detection of colorectal cancer