Dynamics of a many-particle Landau-Zener model: inverse sweep

We consider dynamics of a slowly time-dependent Dicke model, which represents a many-body generalization of the Landau-Zener model. In particular, the model describes narrow Feshbach resonance passage in an ultracold gas of Fermi atoms. Adiabaticity is destroyed when a parameter crosses a critical value, even at very slow sweeping rates of a parameter. The dynamics crucially depends on direction of the sweep. We apply our recent analysis [A.P. Itin, P. Torma, arXiv:0901.4778v1] to the "inverse" sweep through the resonance, corresponding (in a context of Feshbach resonance passage) to dissociation of molecules. On a level of the mean-field approximation, the dynamics is equivalent to a molecular condensate formation from Bose atoms within a two-mode model. Mapping the system to a Painlev\'e equation allows us to calculate deviation from adiabaticity at very slow sweeps analytically.Comment: 3 pages. Submitted to CEWQO 2009 on 14th Februar

Non-adiabacity and large flucutations in a many particle Landau Zener problem

We consider the behavior of an interacting many particle system under slow external driving -- a many body generalization of the Landau-Zener paradigm. We find that a conspiracy of interactions and driving leads to physics profoundly different from that of the single particle limit: for practically all values of the driving rate the particle distributions in Hilbert space are very broad, a phenomenon caused by a strong amplification of quantum fluctuations in the driving process. These fluctuations are 'non-adiabatic' in that even at very slow driving it is exceedingly difficult to push the center of the distribution towards the limit of full ground state occupancy. We obtain these results by a number of complementary theoretical approaches, including diagrammatic perturbation theory, semiclassical analysis, and exact diagonalization.Comment: 25 pages, 16 figure

METHODOLOGICAL APPROACHES TO THE ESTIMATION OF INDIVIDUAL OCCUPATIONAL RISK TAKING INTO ACCOUNT INDICATORS OF LIFE QUALITY

Authors offer to make changes to technique of the estimation of life quality with three blocks of professional and nonprofessional risk factors: index of observance of requirements of sanitary legislations; index of way of life and index of social well-being. The index of observance of requirements of sanitary legislations pays off by result of an expert estimation of a workplace taking into account aggravating (absence of certification of workplaces, industrial inspection; insufficiency of the area and volume of premises; absence of ventilation, repairs, work and rest modes; neediness of good-quality potable water, sanitary-household premises, first-aid sets of the first medical aid; absence of system of informing of workers about an existing professional risk and ways of its decrease etc.) and normalizing factors (presence of complexes intrareplaceable and after a labor shift medical rehabilitations etc.). The index of way of life is offered to be counted taking into account major individual behavioral and biological factors of risk of death rate and invalidity in Russia: smoking, abusing alcohol, irrational food (in particular, the uses less than 400 g vegetables and fruit in day), insufficient physical activity, raised index of weight of a body, psychoemotional stress, raised levels of cholesterol and glucose in whey of blood, arterial pressure, burdened heredity. The index of social well-being pays off as the one-numerical indicator considering social and economic and socially-psychological factors: indicator of size of wages, indicator of satisfaction health, working conditions, work as a whole, life as a whole, an indicator of the individual importance of factors of psychological overload. Authors of article suggest to add an existing technique of an estimation of an indicator of the individual importance of psychological overload factors with indicators actual in modern industrial conditions: socially-psychological adaptation, degree of emotional burning out, quality of life. The estimation of quality of life can serve in this system to one of the most significant after experience medical and biologic criteria of an estimation of an individual professional risk

Transcriptional and Proteomic Analysis of the Aspergillus fumigatus ΔprtT Protease-Deficient Mutant

Aspergillus fumigatus is the most common opportunistic mold pathogen of humans, infecting immunocompromised patients. The fungus invades the lungs and other organs, causing severe damage. Penetration of the pulmonary epithelium is a key step in the infectious process. A. fumigatus produces extracellular proteases to degrade the host structural barriers. The A. fumigatus transcription factor PrtT controls the expression of multiple secreted proteases. PrtT shows similarity to the fungal Gal4-type Zn(2)-Cys(6) DNA-binding domain of several transcription factors. In this work, we further investigate the function of this transcription factor by performing a transcriptional and a proteomic analysis of the ΔprtT mutant. Unexpectedly, microarray analysis revealed that in addition to the expected decrease in protease expression, expression of genes involved in iron uptake and ergosterol synthesis was dramatically decreased in the ΔprtT mutant. A second finding of interest is that deletion of prtT resulted in the upregulation of four secondary metabolite clusters, including genes for the biosynthesis of toxic pseurotin A. Proteomic analysis identified reduced levels of three secreted proteases (ALP1 protease, TppA, AFUA_2G01250) and increased levels of three secreted polysaccharide-degrading enzymes in the ΔprtT mutant possibly in response to its inability to derive sufficient nourishment from protein breakdown. This report highlights the complexity of gene regulation by PrtT, and suggests a potential novel link between the regulation of protease secretion and the control of iron uptake, ergosterol biosynthesis and secondary metabolite production in A. fumigatus

PrtT-Regulated Proteins Secreted by Aspergillus fumigatus Activate MAPK Signaling in Exposed A549 Lung Cells Leading to Necrotic Cell Death

Aspergillus fumigatus is the most commonly encountered mold pathogen of humans, predominantly infecting the respiratory system. Colonization and penetration of the lung alveolar epithelium is a key but poorly understood step in the infection process. This study focused on identifying the transcriptional and cell-signaling responses activated in A549 alveolar carcinoma cells incubated in the presence of A. fumigatus wild-type and ΔPrtT protease-deficient germinating conidia and culture filtrates (CF). Microarray analysis of exposed A549 cells identified distinct classes of genes whose expression is altered in the presence of germinating conidia and CF and suggested the involvement of both NFkB and MAPK signaling pathways in mediating the cellular response. Phosphoprotein analysis of A549 cells confirmed that JNK and ERK1/2 are phosphorylated in response to CF from wild-type A. fumigatus and not phosphorylated in response to CF from the ΔPrtT protease-deficient strain. Inhibition of JNK or ERK1/2 kinase activity substantially decreased CF-induced cell damage, including cell peeling, actin-cytoskeleton damage, and reduction in metabolic activity and necrotic death. These results suggest that inhibition of MAPK-mediated host responses to treatment with A. fumigatus CF decreases cellular damage, a finding with possible clinical implications

Identification of Y-Box Binding Protein 1 As a Core Regulator of MEK/ERK Pathway-Dependent Gene Signatures in Colorectal Cancer Cells

Transcriptional signatures are an indispensible source of correlative information on disease-related molecular alterations on a genome-wide level. Numerous candidate genes involved in disease and in factors of predictive, as well as of prognostic, value have been deduced from such molecular portraits, e.g. in cancer. However, mechanistic insights into the regulatory principles governing global transcriptional changes are lagging behind extensive compilations of deregulated genes. To identify regulators of transcriptome alterations, we used an integrated approach combining transcriptional profiling of colorectal cancer cell lines treated with inhibitors targeting the receptor tyrosine kinase (RTK)/RAS/mitogen-activated protein kinase pathway, computational prediction of regulatory elements in promoters of co-regulated genes, chromatin-based and functional cellular assays. We identified commonly co-regulated, proliferation-associated target genes that respond to the MAPK pathway. We recognized E2F and NFY transcription factor binding sites as prevalent motifs in those pathway-responsive genes and confirmed the predicted regulatory role of Y-box binding protein 1 (YBX1) by reporter gene, gel shift, and chromatin immunoprecipitation assays. We also validated the MAPK-dependent gene signature in colorectal cancers and provided evidence for the association of YBX1 with poor prognosis in colorectal cancer patients. This suggests that MEK/ERK-dependent, YBX1-regulated target genes are involved in executing malignant properties