The NMSSM dark sector constraints

In this paper authors present review of neutralino research results in LHC, cosmological observations and theoretical models. Nowdays there are constraints on the mass of dark matter particle from experiments. The neutralino as a candidate for the dark matter particle in the framework of supersymmetry is considered. There are some discuss advantages for it about the NMSSM model. The properties of neutralino are functions of free parameters of the model. In agreement with the foregoing, constraints on free parameters of the NMSSN are determined

Инфильтрация кожного лоскута местным анестетиком для послеоперационного обезболивания у детей с краниосиностозом после реконструктивных операций

The objective of the study is to evaluate the effectiveness of analgesia by infiltration of the skin flap with local anesthetic in children with craniosynostosis after reconstructive surgery.Materials and subjects. 50 children with craniosynostosis, who underwent reconstructive surgery on skull bones, were divided into two groups based on the method of postoperative anesthesia: in Group 1(experimental), the infiltration of the skin flap was used within multimodal anesthesia, while in Group 2, it was standard parenteral use of analgesic drugs. In the postoperative period, pain severity was assessed by FLACC scales, the amount of opioid and non-opioid analgesics consumed was assessed by the formalized Analgesiс Assessment Scale (FSA), and non-invasive hemodynamic monitoring (BP, HR) was performed.Results. The statistical analysis of the results revealed significant differences between groups in the assessment results of FSA and FLACC scales. In Group 1, the level of postoperative pain was significantly lower compared to Group 2. The amount of opioid and non-opioid analgesics consumed was also significantly lower in Group 1.Conclusion. The use of the infiltration of the skin flap as part of multimodal analgesia in children with craniosynostosis, after reconstructive surgery on skull bones significantly reduces the intensity of pain and the amount of opioid analgesics consumed in the postoperative period.Цель исследования: оценить эффективность обезболивания методом инфильтрации кожного лоскута местным анестетиком у детей с кра- ниосиностозом после реконструктивных операций.Материалы и методы. Проанализированы данные о последовательно оперированных 50 детях с диагнозом «краниосиностоз». Группа 1 (исследуемая) – в составе мультимодальной анальгезии использовали инфильтрацию кожного лоскута; группа 2 (контрольная) ‒ стан- дартное парентеральное применение анальгетических препаратов. В послеоперационном периоде оценивали интенсивность боли по шкале FLACC, количество потребленных наркотических и ненаркотических анальгетиков по формализированной шкале анальгезии (ФША), неинвазивный гемодинамический мониторинг.Результаты. Выявлены достоверные различия в группах по шкалам боли FLACC и ФША. В группе 1 уровень послеоперационной боли был значительно ниже, чем в группе 2. Количество потребленных наркотических и ненаркотических анальгетиков было также значительно ниже в группе 1.Вывод. Инфильтрация кожного лоскута местным анестетиком в составе мультимодальной анальгезии значительно снижает интенсивность боли у детей после реконструктивных операций по поводу краниосиностоза

Molecular Mechanism of a Green-Shifted, pH-Dependent Red Fluorescent Protein mKate Variant

Fluorescent proteins that can switch between distinct colors have contributed significantly to modern biomedical imaging technologies and molecular cell biology. Here we report the identification and biochemical analysis of a green-shifted red fluorescent protein variant GmKate, produced by the introduction of two mutations into mKate. Although the mutations decrease the overall brightness of the protein, GmKate is subject to pH-dependent, reversible green-to-red color conversion. At physiological pH, GmKate absorbs blue light (445 nm) and emits green fluorescence (525 nm). At pH above 9.0, GmKate absorbs 598 nm light and emits 646 nm, far-red fluorescence, similar to its sequence homolog mNeptune. Based on optical spectra and crystal structures of GmKate in its green and red states, the reversible color transition is attributed to the different protonation states of the cis-chromophore, an interpretation that was confirmed by quantum chemical calculations. Crystal structures reveal potential hydrogen bond networks around the chromophore that may facilitate the protonation switch, and indicate a molecular basis for the unusual bathochromic shift observed at high pH. This study provides mechanistic insights into the color tuning of mKate variants, which may aid the development of green-to-red color-convertible fluorescent sensors, and suggests GmKate as a prototype of genetically encoded pH sensors for biological studies

Infiltrative analgesia of the skin flap in children with craniosynostosis after reconstructive surgery on skull bones

The objective of the study is to evaluate the effectiveness of analgesia by infiltration of the skin flap with local anesthetic in children with craniosynostosis after reconstructive surgery.Materials and subjects. 50 children with craniosynostosis, who underwent reconstructive surgery on skull bones, were divided into two groups based on the method of postoperative anesthesia: in Group 1(experimental), the infiltration of the skin flap was used within multimodal anesthesia, while in Group 2, it was standard parenteral use of analgesic drugs. In the postoperative period, pain severity was assessed by FLACC scales, the amount of opioid and non-opioid analgesics consumed was assessed by the formalized Analgesiс Assessment Scale (FSA), and non-invasive hemodynamic monitoring (BP, HR) was performed.Results. The statistical analysis of the results revealed significant differences between groups in the assessment results of FSA and FLACC scales. In Group 1, the level of postoperative pain was significantly lower compared to Group 2. The amount of opioid and non-opioid analgesics consumed was also significantly lower in Group 1.Conclusion. The use of the infiltration of the skin flap as part of multimodal analgesia in children with craniosynostosis, after reconstructive surgery on skull bones significantly reduces the intensity of pain and the amount of opioid analgesics consumed in the postoperative period

The diagnosis and treatment of neonates and infants with posterior urethral valves

In January 2009 to December 2013, the N.F. Filatov Children’s City Clinical Hospital (CCCH) Thirteen gave 357 consultations to pregnant women with fetal urinary tract abnormalities and identified the signs of infravesical obstruction in 27 fetuses of 28–32 weeks of gestation. During the first 24 hours of life, these babies were urgently admitted from maternity hospitals to the Unit for Surgery of Neonates and Premature Babies for further examination and treatment. The diagnosis was verified by miction cystourethrography that showed posterior urethral enlargement. Comprehensive X-ray urological examination and blood biochemical tests were carried out in the unit to evaluate renal function. For additional assessment of the latter, urinary enzymes and β2-microglobulins were examined and biochemical urinalysis was performed from September 2012. All the infants underwent transurethral resection of a posterior urethral valve. Later on, the babies were followed up and treated in an outpatient setting in the Nephrourological Center, N.F. Filatov CCCH Thirteen. The duration of the follow-up was 1 to 4 years. Analysis of the long-term results of endoscopic correction of ureterovesical segment abnormality shows that the use of mini-invasive treatment options in neonates and infants makes it possible to recover urinary tract urodynamics and bladder evacuation function, to reduce the number of infectious complications, and to prevent reflux nephropathy and chronic kidney disease.</em