Genetic nomenclature for new lymphocyte antigens controlled by the I region of the H-2 complex

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46731/1/251_2005_Article_BF01564059.pd

Evidence for a third, Ir -associated histocompatibility region in the H-2 complex of the mouse

Skin grafts transplanted from B10.HTT donors onto (A.TL × B10)F 1 recipients are rapidly rejected despite the fact that the B10.HTT and A.TL strains should be carrying the same H-2 chromosomes and that both the donor and the recipient contain the B10 genome. The rejection is accompanied by a production of cytotoxic antibodies against antigens controlled by the Ir region of the H-2 complex. These unexpected findings are interpreted as evidence for a third histocompatibility locus in the H-2 complex, H-2I , located in the Ir region close to H-2K . The B10.HTT and A.TL strains are postulated to differ at this hypothetical locus, and the difference between the two strains is explained as resulting from a crossing over between the H-2 t1 and H-2 s chromosomes in the early history of the B10.HTT strain. The H-2 genotypes of the B10.HTT and A.TL strains are assumed to be H-2K s Ir s / k Ss k H-2D d and H-2K s Ir k Ss k H-2D d , respectively. Thus, the H-2 chromosomes of the two strains differ only in a portion of the Ir region, including the H-2I locus. The B10.HTT( H-2 tt ) and B10.S(7R)( H-2 th ) strains differ in a relatively minor histocompatibility locus, possibly residing in the Tla region outside of the H-2 complex.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46727/1/251_2005_Article_BF01564045.pd

Partial characterization of Ia antigens from murine lymphoid cells

Congenic anti-Ia antisera were used to bind radiolabelled Ia antigens from cells of various strains of mice of known H-2 haplotype. The results indicate that Ia antigens are proteins of molecular weight 30,000 to 35,000 daltons. The Ia antigens are distinct from known H-2 antigens as judged by independent immunoprecipitation as well as by molecular weight. Ia antigens are synthesized by, and are present on the surface of lymphoid cells as evidenced by incorporation studies using 3 H-leucine and enzymatic radioiodination of cells, respectively. Tissue distribution of cell surface Ia suggests that Ia antigens are on B cells. Ia antigens were detected in the incubation media of 3 H-leucine labeled splenocytes suggesting that antigens may be secreted.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46728/1/251_2005_Article_BF01564048.pd